14-3-3 (pSer185) antibody

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Sheep anti 14-3-3 (pSer185)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1049 WB* 0.1 mg
Sheep anti 14-3-3 (pSer185) antibody recognizes 14-3-3 isoforms beta and zeta when phosphorylated at serine 185. The members of the 14-3-3 family of proteins are highly conserved and are involved in multiple signalling pathways. The phosphorylated forms of beta and zeta 14-3-3 are found endogenously in brain tissue.

Product Details

Target Species
Sheep
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Bovine
Mouse
Rat
Rabbit
Mammals Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Phosphorylated 14-3-3 were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to 14-3-3 beta and zeta phosphorylated at serine 185.

Peptide sequence: EILNSPEKAC
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from the date of despatch.

More Information

UniProt
P29361 Related reagents
O77642 Related reagents
P68253 Related reagents
P68251 Related reagents
P62262 Related reagents
Entrez Gene
SFN Related reagents
LOC443300 Related reagents
GO Terms
GO:0005576 extracellular region
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0019904 protein domain specific binding
GO:0042470 melanosome
GO:0005159 insulin-like growth factor receptor binding
GO:0045664 regulation of neuron differentiation
GO:0048167 regulation of synaptic plasticity
Regulatory
For research purposes only

Applications of 14-3-3 (pSer185) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1 1/100 1/300
  1. 1 For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1049 detects a band of approximately 30kDa.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti 14-3-3 Isoform Panel PAN017 25 µl X 7 E* P* WB*

Product Specific References

Further Reading

  1. Aitken, A. et al. (1995) 14-3-3 alpha and delta are the phosphorylated forms of Raf-activating 14-3-3 beta and zeta. In vivo stoichiometric phosphorylation in brain at Ser-Pro-Glu-Lys Motif.
    J. Biol. Chem. 270: 5706 – 5709.