14-3-3 Epsilon antibody

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Rabbit anti 14-3-3 Epsilon (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1048 E P* WB 0.1 ml
Rabbit anti 14-3-3 antibody recognizes the acetylated N-terminal of 14-3-3 epsilon, a member of the 14-3-3 family which consists of 30 kDa proteins involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein.

14-3-3 epsilon is expressed in all mammals. It is deleted in small cell lung cancers disrupting the G checkpoint (Wang, Z. et al. 2011). During apoptosis, cleavage of 14-3-3 protein by caspase-3 increases the affinity between bad and Bcl-x(L). When bound by ERK, 14-3-3 epsilon is thought to deactivate heat shock factor 1 in cells recovering from heat shock. It is also a potential suicide susceptibility gene.

Rabbit anti 14-3-3 antibody may not react with recombinant proteins that are not N-acetylated.

Product Details

Target Species
Sheep
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Bovine
Chicken
Mouse
Rabbit
Rat
Mammals Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Antiserum Preparation
Antisera to sheep 14-3-3 epsilon N-terminus were raised by repeated immunisations of rabbits with highly purified antigen.
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 epsilon.

Peptide sequence: Ac.MDDREDLVYQAKC

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P62262 Related reagents
Entrez Gene
LOC443300 Related reagents
GO Terms
GO:0019904 protein domain specific binding
GO:0042470 melanosome
Regulatory
For research purposes only

Applications of 14-3-3 Epsilon antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Paraffin 1 1/400
Western Blotting 1/3000
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Normal brain.
Western Blotting
Rabbit anti 14-3-3 antibody detects a band/s of approximately 30kDa in HEK293 cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti 14-3-3 Isoform Panel PAN017 25 µl X 7 E* P* WB*
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Application Based External Images

Immunohistology - Paraffin

Product Specific References

References for 14-3-3 Epsilon antibody

  1. Martin, H. et al. (1993) Antibodies against the major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein.
    FEBS Lett. 331 (3): 296-303.
  2. Wang, Z. et al. (2011) The prognostic value of 14-3-3 isoforms in vulvar squamous cell carcinoma cases: 14-3-3β and ε are independent prognostic factors for these tumors.
    PLoS One. 6: e24843.
  3. Taurino, F. et al. (2014) Short-term type-1 diabetes differentially modulates 14-3-3 proteins in rat brain and liver.
    Eur J Clin Invest. 44 (4): 350-8.
  4. De, S. et al. (2012) Expression of 14-3-3 protein isoforms in mouse oocytes, eggs and ovarian follicular development
    BMC Res Notes. 5: 57.

Further Reading

  1. Aitken, A. (2006) 14-3-3 proteins: a historic overview.
    Semin Cancer Biol. 16 (3): 162-72.