Rabbit anti serotonin antibody recognizes serotonin, also known as 5-hydroxytryptamine, or 5-HT. Serotonin is a monoamine neurotransmitter synthesised in serotonergic neurons in the central nervous system and enterochromaffin cells in the gastrointestinal tract. Serotonin has broad activities in the brain. Genetic variation in serotonin receptors and the serotonin transporter (which facilitates reuptake of serotonin into presynapses) has been implicated in neurological diseases. Drugs targeting serotonin-induced pathways are being used in the treatment of many psychiatric disorders and one focus of clinical research is the influence of genetics on serotonin action and metabolism in psychiatric settings.
- Species Cross-Reactivity
Target Species Cross Reactivity Human Rat
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to serotonin were raised by repeated immunisation of rabbits with highly purified antigen. Whole serum is supplied.
- Preservative Stabilisers
0.1% Sodium Azide
- Serotonin coupled to BSA.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 18 months from date of despatch.
- For research purposes only
Applications of Serotonin antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen 1|
|Immunohistology - Paraffin 2||1/500||1/2000|
- 1Gluteraldehyde (2- 5 %) should be included in the fixative. A metabisulphite reduction step is required. * For frozen sections, a borohydride reduction step is also required.
- 2For paraffin wax sections, it is recommended that a brief 10 min pre-treatment in trypsin solution (1 mg/ml in PBS) is included to enhance immunostaining. Gluteraldehyde (2- 5 %) should be included in the fixative. A metabisulphite reduction step is required.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
|Description||Product Code||Pack Size||Applications||List Price||Quantity|
|Sheep anti Rabbit IgG:Biotin||2AB02B||1 ml||C E P WB|
|Goat anti Rabbit IgG (Fc):Biotin||STAR121B||1 mg||E WB|
|Goat anti Rabbit IgG (Fc):FITC||STAR121F||1 mg||F|
|Goat anti Rabbit IgG (Fc):HRP||STAR121P||1 mg||E WB|
|Goat anti Rabbit IgG (H/L):HRP||STAR124P||1 mg||C E WB|
|Sheep anti Rabbit IgG:FITC||STAR34B||1 mg||C F|
|Sheep anti Rabbit IgG:RPE||STAR35A||1 ml||F|
|Sheep anti Rabbit IgG:DyLight®488||STAR36D488GA||0.1 mg||F IF WB|
|Sheep anti Rabbit IgG:DyLight®549||STAR36D549GA||0.1 mg||F IF|
|Sheep anti Rabbit IgG:DyLight®649||STAR36D649GA||0.1 mg||F IF|
|Sheep anti Rabbit IgG:DyLight®680||STAR36D680GA||0.1 mg||F IF WB|
|Sheep anti Rabbit IgG:Dylight®800||STAR36D800GA||0.1 mg||F IF WB|
Product Specific References
References for Serotonin antibody
Dicke, U. et al. (1997) 5-HT-like immunoreactivity in the brains of plethodontid and salamandrid salamanders (Hydromantes italicus, Hydromantes genei, Plethodon jordani, Desmognathus ochrophaeus, Pleurodeles waltl): an immunohistochemical and biocytin double-labelling study.
Cell Tissue Res. 287 (3): 513-23.