TGN38 antibody

100% Secure

Rabbit anti Rat TGN38

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1597 IF* WB 0.1 ml
Rabbit anti Rat TGN38 antibody recognizes a glycoprotein designated TGN38 that is found primarily in the Trans-golgi network, and has been found to be an excellent marker for this cellular organelle.

TGN38 is likely to have a role in intracellular transport. It is the homologue of human TGN46.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Antiserum Preparation
Antisera to TGN38 were raised by repeated immunisations of rabbits with highly purified antigen.
Preservative Stabilisers
0.09% Sodium Azide, 1% Bovine Serum Albumin.
Immunogen
Recombinant protein corresponding to extracellular domain of TGN38.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P19814 Related reagents
Entrez Gene
Tgoln1 Related reagents
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0005768 endosome
GO:0005794 Golgi apparatus
GO:0006895 Golgi to endosome transport
Regulatory
For research purposes only

Applications of TGN38 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1 1/50 1/100
Western Blotting 1/500 1/1000
  1. 1Fixation with 3% paraformaldehyde or methanol is recommended.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

References for TGN38 antibody

  1. Ponnambalam, S. et al. (1996) Primate homologues of rat TGN38: primary structure, expression and functional implications.
    J Cell Sci. 109 ( Pt 3): 675-85.
  2. Luzio, J.P. et al. (1990) Identification, sequencing and expression of an integral membrane protein of the trans-Golgi network (TGN38).
    Biochem J. 270 (1): 97-102.
  3. Banerji, S. et al. (2010) Oxysterol binding protein-dependent activation of sphingomyelin synthesis in the golgi apparatus requires phosphatidylinositol 4-kinase IIα.
    Mol Biol Cell. 21 (23): 4141-50.
  4. Park, B.C. et al. (2015) The clathrin-binding and J-domains of GAK support the uncoating and chaperoning of clathrin by Hsc70 in the brain.
    J Cell Sci. 128 (20): 3811-21.
  5. Chen, A. et al. (2012) Regional and subcellular distribution of the receptor-targeting protein PIST in the rat central nervous system.
    J Comp Neurol. 520 (5): 889-913.
  6. González C et al. (2016) Axons provide the secretory machinery for trafficking of voltage-gated sodium channels in peripheral nerve.
    Proc Natl Acad Sci U S A. 113 (7): 1823-8.
  7. Zuidscherwoude, M. et al. (2015) The tetraspanin web revisited by super-resolution microscopy.
    Sci Rep. 5: 12201.