tau (pSer416) antibody

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Rabbit anti tau (pSer416)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1874 WB* 0.1 ml
Rabbit anti Rat tau (pSer416) antibody recognizes microtubule-associated protein tau, also known as neurofibrillary tangle protein and paired helical filament-tau (PHF-tau), when phosphorylated at serine 416. Tau is a heterogeneous group of proteins that are involved in microtubule assembly. In healthy brain tissue tau is localised to the axons of neurons. Hyperphosphorylated tau is a major component of paired helical filaments in Alzheimer's disease brain. Serine 416 is a major phosphorylation site.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human Expected from Sequence
Mouse Expected from Sequence
Bovine Expected from Sequence
Dog Expected from Sequence
Primate Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Tau were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
10mM Hepes pH7.5, 150mM NaCl
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.01% Bovine Serum Albumin
50% Glycerol
Immunogen
Phosphopeptide corresponding to amino acid sequence surrounding the phosphorylated serine 416 of rat Tau.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P19332 Related reagents
Entrez Gene
Mapt Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0000226 microtubule cytoskeleton organization
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0005874 microtubule
GO:0007026 negative regulation of microtubule depolymerization
GO:0007584 response to nutrient
GO:0008017 microtubule binding
GO:0010033 response to organic substance
GO:0019901 protein kinase binding
GO:0019904 protein domain specific binding
GO:0030182 neuron differentiation
GO:0031116 positive regulation of microtubule polymerization
GO:0044295 axonal growth cone
GO:0045298 tubulin complex
GO:0045773 positive regulation of axon extension
Regulatory
For research purposes only

Applications of tau (pSer416) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1 1/1000
  1. 1For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1874 detects bands of approximately 59, 65 and 68kDa in rat brain.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*