Stem Cell Factor antibody

Rabbit anti Rat Stem Cell Factor

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Stem Cell Factor

Product Code Applications Pack Size List Price Your Price Qty
AAR44
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SDS Safety Datasheet SDS
E FN WB 0.1 mg loader
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Rabbit anti Rat Stem Cell Factor antibody specifically recognizes rat stem cell factor (SCF), a type I transmembrane protein and haematopoietic growth factor which acts in synergy with other growth factors, and signals through the receptor tyrosine kinase c-Kit (CD117).

The interaction between SCF and c-Kit regulates developmental and functional processes of haematopoietic progenitor cells, mast cells, myelanocytes and germ cells, and plays an important role in follicular development. The interaction between SCF and c-Kit is associated with the progression and invasiveness of some tumours, including melanomas.

Target Species
Rat
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antiserum to rat SCF was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant rat SCF.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Functional Assays 0.08 0.12ug/ml
Western Blotting 0.1 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Synonyms
SCF
RRID
AB_10709280
UniProt
P21581
Entrez Gene
Kitlg
GO Terms
GO:0005886 plasma membrane
GO:0001541 ovarian follicle development
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0005737 cytoplasm
GO:0005856 cytoskeleton
GO:0008083 growth factor activity
View more products with STEM CELL FACTOR specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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