Stem Cell Factor antibody
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Rabbit anti Rat Stem Cell Factor
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Stem Cell Factor
Antibody Quality and 
Unrivaled 
| Rabbit anti Rat Stem Cell Factor antibody specifically recognizes rat stem cell factor (SCF), a type I transmembrane protein and haematopoietic growth factor which acts in synergy with other growth factors, and signals through the receptor tyrosine kinase c-Kit (CD117). The interaction between SCF and c-Kit regulates developmental and functional processes of haematopoietic progenitor cells, mast cells, myelanocytes and germ cells, and plays an important role in follicular development. The interaction between SCF and c-Kit is associated with the progression and invasiveness of some tumours, including melanomas. |
- Target Species
- Rat
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antiserum to rat SCF was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant rat SCF.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5 | 2.0ug/ml |
| Functional Assays | |
0.08 | 0.12ug/ml |
| Western Blotting | |
0.1 | 0.2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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- Synonyms
- SCF
- RRID
- AB_10709280
- UniProt
- P21581
- Entrez Gene
- Kitlg
- GO Terms
- GO:0005886 plasma membrane
- GO:0001541 ovarian follicle development
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0005737 cytoplasm
- GO:0005856 cytoskeleton
- GO:0008083 growth factor activity
View more products with STEM CELL FACTOR specificity
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