RAF-1 (pSer642) antibody
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Rabbit anti Rat Raf-1 (pSer642) antibody recognizes Raf-1, also known as RAF proto-oncogene serine/threonine-protein kinase and proto-oncogene c-RAF (cRaf), when phosphorylated at serine 642. Raf-1is a ubiquitously expressed ~74 kDa member of the serine/threonine protein kinase family. Raf-1 is a MAP kinase kinase kinase and participates in the transduction of mitogenic signals from receptors on the cell membrane to the nucleus. Raf-1 is activated through phosphorylation by Ras and in turn phosphorylates MEK which activates ERK. ERK when activated regulates the expression of genes involved in cell cycle, differentiation, migration and apoptosis. As such Raf-1 is an important clinical target in cancer.
Raf-1 activity can be downregulated by phosphorylation at serine residues 301 and 642.
Raf-1 activity can be downregulated by phosphorylation at serine residues 301 and 642.
Product Details
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Expected from Sequence Bovine Expected from Sequence Chicken Expected from Sequence Mouse Expected from Sequence Dog Expected from Sequence Primate Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to rat Raf-1 (pSer 642) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared affinity chromatography.
- Buffer Solution
- 10mM Hepes pH7.5
150mM NaCl - Preservative Stabilisers
0.09% Sodium Azide (NaN3) 0.01% Bovine Serum Albumin 50% Glycerol - Immunogen
- Phosphopeptide corresponding to animo acid sequence surrounding phosphorylated Ser 642 of rat Raf-1.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P11345
- Entrez Gene
- Raf1
- GO Terms
- GO:0000165 MAPKKK cascade
- GO:0001666 response to hypoxia
- GO:0005829 cytosol
- GO:0004709 MAP kinase kinase kinase activity
- GO:0046872 metal ion binding
- GO:0005524 ATP binding
- GO:0005626 insoluble fraction
- GO:0006468 protein phosphorylation
- GO:0007507 heart development
- GO:0031434 mitogen-activated protein kinase kinase binding
- GO:0046982 protein heterodimerization activity
- Regulatory
- For research purposes only
Applications of RAF-1 (pSer642) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1237 detects a band of approximately 74kDa.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Product Specific References
References for RAF-1 (pSer642) antibody
-
Gollob, J.A. et al. (2006) Role of Raf kinase in cancer: therapeutic potential of targeting the Raf/MEK/ERK signal transduction pathway.
Semin Oncol. 33 (4): 392-406. -
Dougherty, M.K. et al. (2005) Regulation of Raf-1 by direct feedback phosphorylation.
Mol Cell. 17 (2): 215-24.
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