RAF-1 (pSer642) antibody

Raf-1 activity can be downregulated by phosphorylation at serine residues 301 and 642.
Product Details
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Expected from Sequence Bovine Expected from Sequence Chicken Expected from Sequence Mouse Expected from Sequence Dog Expected from Sequence Primate Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to rat Raf-1 (pSer 642) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared affinity chromatography.
- Buffer Solution
- 10mM Hepes pH7.5
150mM NaCl - Preservative Stabilisers
0.09% Sodium Azide (NaN3) 0.01% Bovine Serum Albumin 50% Glycerol - Immunogen
- Phosphopeptide corresponding to animo acid sequence surrounding phosphorylated Ser 642 of rat Raf-1.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P11345
- Entrez Gene
- Raf1
- GO Terms
- GO:0000165 MAPKKK cascade
- GO:0001666 response to hypoxia
- GO:0005829 cytosol
- GO:0004709 MAP kinase kinase kinase activity
- GO:0046872 metal ion binding
- GO:0005524 ATP binding
- GO:0005626 insoluble fraction
- GO:0006468 protein phosphorylation
- GO:0007507 heart development
- GO:0031434 mitogen-activated protein kinase kinase binding
- GO:0046982 protein heterodimerization activity
- Regulatory
- For research purposes only
Applications of RAF-1 (pSer642) antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Western Blotting | 1/1000 |
- Western Blotting
- AHP1237 detects a band of approximately 74kDa.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
![]() |
|
Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
![]() |
|
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
![]() |
|
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
![]() |
|
Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
![]() |
|
Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
![]() |
|
Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Product Specific References
References for RAF-1 (pSer642) antibody
-
Gollob, J.A. et al. (2006) Role of Raf kinase in cancer: therapeutic potential of targeting the Raf/MEK/ERK signal transduction pathway.
Semin Oncol. 33 (4): 392-406. -
Dougherty, M.K. et al. (2005) Regulation of Raf-1 by direct feedback phosphorylation.
Mol Cell. 17 (2): 215-24.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra