RAF-1 (pSer642) antibody

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Rabbit anti RAF-1 (pSer642)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1237
Datasheet
WB
MSDS
0.1 ml loader

Rabbit anti Rat Raf-1 (pSer642) antibody recognizes Raf-1, also known as RAF proto-oncogene serine/threonine-protein kinase and proto-oncogene c-RAF (cRaf), when phosphorylated at serine 642. Raf-1is a ubiquitously expressed ~74 kDa member of the serine/threonine protein kinase family. Raf-1 is a MAP kinase kinase kinase and participates in the transduction of mitogenic signals from receptors on the cell membrane to the nucleus. Raf-1 is activated through phosphorylation by Ras and in turn phosphorylates MEK which activates ERK. ERK when activated regulates the expression of genes involved in cell cycle, differentiation, migration and apoptosis. As such Raf-1 is an important clinical target in cancer.

Raf-1 activity can be downregulated by phosphorylation at serine residues 301 and 642.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human Expected from Sequence
Bovine Expected from Sequence
Chicken Expected from Sequence
Mouse Expected from Sequence
Dog Expected from Sequence
Primate Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to rat Raf-1 (pSer 642) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared affinity chromatography.
Buffer Solution
10mM Hepes pH7.5
150mM NaCl
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
0.01%Bovine Serum Albumin
50% Glycerol
Immunogen
Phosphopeptide corresponding to animo acid sequence surrounding phosphorylated Ser 642 of rat Raf-1.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch.

More Information

UniProt
P11345 Related reagents
Entrez Gene
Raf1 Related reagents
GO Terms
GO:0000165 MAPKKK cascade
GO:0001666 response to hypoxia
GO:0005829 cytosol
GO:0004709 MAP kinase kinase kinase activity
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0005626 insoluble fraction
GO:0006468 protein phosphorylation
GO:0007507 heart development
GO:0031434 mitogen-activated protein kinase kinase binding
GO:0046982 protein heterodimerization activity
Regulatory
For research purposes only

Applications of RAF-1 (pSer642) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1237 detects a band of approximately 74kDa.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B C E P WB 1 ml loader
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Product Specific References

References for RAF-1 (pSer642) antibody

  1. Gollob, J.A. et al. (2006) Role of Raf kinase in cancer: therapeutic potential of targeting the Raf/MEK/ERK signal transduction pathway.
    Semin Oncol. 33 (4): 392-406.
  2. Dougherty, M.K. et al. (2005) Regulation of Raf-1 by direct feedback phosphorylation.
    Mol Cell. 17 (2): 215-24.