Prolactin antibody

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Rabbit anti Rat Prolactin

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: Prolactin

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Secondary Antibodies
References (3)
Applications
Storage Information
Batch/Lot Specific Datasheets

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7770-5004G Datasheet Datasheet SDS SDS	C E	0.25 ml		Log in	Get Quote
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Rabbit anti Rat prolactin antibody recognizes prolactin, a secreted protein with hormonal activity, which functions by binding to the prolactin receptor and positively regulating epithelial cell proliferation. Prolactin acts primarily on the mammary gland by promoting lactation, and has been shown to promote prostate epithelial cell proliferation(Ahonen et al. 1999). Prolactin may also facilitate decidual growth and placentation. Prolactin belongs to the somatotropin/prolactin family.

Target Species: Rat
Product Form: Purified IgG - liquid
Antiserum Preparation: Antisera to prolactin were raised by repeated immunisations of rabbit with highly purified antigen. IgG fraction prepared by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Purified rat prolactin from pituitary glands.
Approx. Protein Concentrations: IgG concentration 5.0mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1/50	1/200
Immunohistology - Frozen

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for Prolactin antibody

Alam, S.M. et al. (2006) The rat prolactin gene family locus: species-specific gene family expansion.
Mamm Genome. 17 (8): 858-77.
Gourdji, D. & Laverrière, J.N. (1994) The rat prolactin gene: a target for tissue-specific and hormone-dependent transcription factors.
Mol Cell Endocrinol. 100 (1-2): 133-42.
Farris, G.M. et al. (2007) Recombinant rat and mouse growth hormones: risk assessment of carcinogenic potential in 2-year bioassays in rats and mice.
Toxicol Sci. 97: 548-61.

Synonyms: PRL
RRID: AB_2237668
UniProt: P01237
Entrez Gene: Prl
GO Terms: GO:0005576 extracellular region; GO:0005148 prolactin receptor binding; GO:0005179 hormone activity; GO:0030141 stored secretory granule; GO:0007567 parturition; GO:0007584 response to nutrient; GO:0007595 lactation; GO:0007623 circadian rhythm; GO:0042493 response to drug; View More GO Terms; GO:0014070 response to organic cyclic compound; GO:0030278 regulation of ossification; GO:0032355 response to estradiol stimulus; GO:0032496 response to lipopolysaccharide; GO:0032870 cellular response to hormone stimulus; GO:0033555 multicellular organismal response to stress; GO:0042698 ovulation cycle; GO:0045471 response to ethanol; GO:0050679 positive regulation of epithelial cell proliferation