Nmdar NR2B (pTyr1336) antibody

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Rabbit anti NMDA Receptor NR2B (pTyr1336)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP978 WB* 0.1 ml
Rabbit anti Rat NMDA receptor NR2B (pTyr1336) antibody recognizes NMDA receptor NR2B, also known as glutamate receptor ionotropic, NMDA 2B (GluN2B), glutamate [NMDA] receptor subunit epsilon-2 and N-methyl D-aspartate receptor subtype 2B (NMDAR2B), when phosphorylated at tyrosine 1336.

Receptors for NMDA belong to a group of ionotropic glutamate receptors which play a key role in the mediation of glutamate neurotransmission within the mammalian central nervous system (CNS), including involvement in memory and learning processes. Several antagonists and agonists of NMDA receptors (NMDAR) have been identified, including the glutamate analogue Homoquinolinic acid, which displays a higher affinity for NR2B-containing NMDAR. Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage-dependent Mg2+ blockade and high Ca2+ permeability and the phosphorylation of tyrosine 1336 is thought to potentiate NMDAR-dependent Ca2+ influx.

The involvement of NMDAR in the CNS has become a focus area for neurodegenerative diseases such as Alzheimer’s disease (Popke 2003) and also epilepsy and ischemic neuronal cell death.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Monkey Expected from Sequence
Human Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to rat NMDA receptor NR2B subunit were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
10mM Hepes pH7.5
Preservative Stabilisers
0.09%Sodium Azide
0.01%Bovine Serum Albumin
50%Glycerol
Immunogen
Synthetic phosphopeptide corresponding to an amino acid sequence within the NR2B subunit of the NMDA receptor, which includes phosphorylated Tyr1336.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

UniProt
Q00960 Related reagents
Entrez Gene
Grin2b Related reagents
GO Terms
GO:0001508 regulation of action potential
GO:0001975 response to amphetamine
GO:0050839 cell adhesion molecule binding
GO:0004972 N-methyl-D-aspartate selective glutamate receptor activity
GO:0005234 extracellular-glutamate-gated ion channel activity
GO:0007616 long-term memory
GO:0008013 beta-catenin binding
GO:0008144 drug binding
GO:0008270 zinc ion binding
GO:0008306 associative learning
GO:0051707 response to other organism
GO:0009743 response to carbohydrate stimulus
GO:0010350 cellular response to magnesium starvation
GO:0010942 positive regulation of cell death
GO:0014049 positive regulation of glutamate secretion
GO:0014069 postsynaptic density
GO:0016600 flotillin complex
GO:0017146 N-methyl-D-aspartate selective glutamate receptor complex
GO:0021766 hippocampus development
GO:0021987 cerebral cortex development
GO:0030018 Z disc
GO:0030054 cell junction
GO:0030288 outer membrane-bounded periplasmic space
GO:0031749 D2 dopamine receptor binding
GO:0032026 response to magnesium ion
GO:0034097 response to cytokine stimulus
GO:0035235 ionotropic glutamate receptor signaling pathway
GO:0035255 ionotropic glutamate receptor binding
GO:0042165 neurotransmitter binding
GO:0042220 response to cocaine
GO:0042734 presynaptic membrane
GO:0043083 synaptic cleft
GO:0043113 receptor clustering
GO:0043195 terminal button
GO:0043197 dendritic spine
GO:0043408 regulation of MAPKKK cascade
GO:0045211 postsynaptic membrane
GO:0045471 response to ethanol
GO:0046982 protein heterodimerization activity
GO:0048169 regulation of long-term neuronal synaptic plasticity
GO:0048511 rhythmic process
GO:0050806 positive regulation of synaptic transmission
GO:0051592 response to calcium ion
GO:0051597 response to methylmercury
GO:0051602 response to electrical stimulus
GO:0060416 response to growth hormone stimulus
GO:0060992 response to fungicide
GO:0071230 cellular response to amino acid stimulus
GO:0071287 cellular response to manganese ion
GO:0071359 cellular response to dsRNA
GO:0071363 cellular response to growth factor stimulus
GO:0071396 cellular response to lipid
GO:0071407 cellular response to organic cyclic compound
GO:0071445 cellular response to protein stimulus
Regulatory
For research purposes only

Applications of Nmdar NR2B (pTyr1336) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1 1/1000
  1. 1 For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP978 detects a band of approximately 180kDa in rat hippocampus cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Product Specific References

References for Nmdar NR2B (pTyr1336) antibody

  1. Takasu, M.A. et al. (2002) Modulation of NMDA receptor-dependent calcium influx and gene expression through EphB receptors.
    Science. 295 (5554): 491-5.
  2. Rosenblum, K. et al. (1996) Long-term potentiation increases tyrosine phosphorylation of the N-methyl-D-aspartate receptor subunit 2B in rat dentate gyrus in vivo.
    Proc Natl Acad Sci U S A. 93 (19): 10457-60.

Further Reading

  1. Ishii, T. et al. (1993) Molecular characterization of the family of the N-methyl-D-aspartate receptor subunits.
    J Biol Chem. 268 (4): 2836-43.
  2. Popke, E.J. (2003) From anticholinesterase toxicity to Alzheimer's disease: important interactions of cholinergic and NMDA receptor systems.
    Toxicol Sci. 72 (2): 185-7.