MIP-1 Alpha antibody

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Rabbit anti Rat MIP-1 Alpha:Biotin

Rabbit anti Rat MIP-1 Alpha

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAR30B E WB 50 µg
AAR30 E FN WB 0.1 mg
Rabbit anti Rat MIP-1 alpha antibody recognizes rat macrophage inflammatory protein-1 alpha (MIP-1 alpha), also known as CCL3.

MIP-1 alpha has a molecular weight of approximately 7.8kD, which is produced by activated macrophages. MIP-1 alpha has been shown to be involved in the inflammatory response of blood monocytes and tissue macrophages.

Product Details

Target Species
Rat
Product Form
Purified IgG conjugated to Biotin - lyophilised
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 1.0 ml sterile PBS containing 0.1% Bovine Serum Albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Reconstitution
Reconstitute with 0.1 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Antiserum Preparation
Antisera to rat MIP-1 alpha were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None Present
Preservative Stabilisers
None present
Immunogen
Recombinant rat MIP-1 alpha.
Approx. Protein Concentrations
IgG concentration 50 ug/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. The addition of 0.09% sodium azide is recommended for long term storage.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. The addition of 0.09% sodium azide is recommended for long term storage.

N.B. For functional studies do not add sodium azide.
Shelf Life
12 months from date of reconstitution.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P50229 Related reagents
Entrez Gene
Ccl3 Related reagents
GO Terms
GO:0005615 extracellular space
GO:0005625 soluble fraction
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007204 elevation of cytosolic calcium ion concentration
GO:0008009 chemokine activity
GO:0008201 heparin binding
GO:0042493 response to drug
GO:0030593 neutrophil chemotaxis
GO:0045672 positive regulation of osteoclast differentiation
Regulatory
For research purposes only

Applications of MIP-1 Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.15ug/ml 0.30ug/ml
Western Blotting 0.1ug/ml 0.2ug/ml
ELISA 0.5ug/ml
Functional Assays 8.0ug/ml 10.0ug/ml
Western Blotting 0.1ug/ml 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*