Metabotropic Glutamate Receptor 1a antibody

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Rabbit anti Rat Metabotropic Glutamate Receptor 1a

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1871 C WB 0.1 ml
Rabbit anti Rat metabotropic glutamate receptor 1a antibody recognizes rat metabotropic glutamate receptor 1a (mGluR1a). The metabotropic glutamate receptors have been organised into three groups. mGluR5 and mGluR1 make up group I which, when activated, stimulates phospholipase C and activates protein kinase C to hydrolyse phosphoinositide phospholipids in the cell membrane. Group I mGluRs, but not other groups, are activated by 3, 5-dihydroxyphenylglycine (DHPG).

Product Details

Target Species
Rat
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to metabotropic glutamate receptor 1a were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
10mM HEPES pH7.5, 150mM NaCl
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.01% Bovine Serum Albumin
50% Glycerol
Immunogen
Peptide corresponding to amino acids 1115-1130 from the C-terminal region of rat mGluR1a.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P23385 Related reagents
Entrez Gene
Grm1 Related reagents
GO Terms
GO:0005515 protein binding
GO:0001639 PLC activating metabotropic glutamate receptor activity
GO:0004930 G-protein coupled receptor activity
GO:0005887 integral to plasma membrane
GO:0007206 activation of phospholipase C activity by metabotropic glutamate receptor signaling pathway
GO:0019722 calcium-mediated signaling
GO:0014048 regulation of glutamate secretion
GO:0014069 postsynaptic density
GO:0019233 sensory perception of pain
GO:0030424 axon
GO:0042734 presynaptic membrane
GO:0043197 dendritic spine
GO:0051482 elevation of cytosolic calcium ion concentration involved in G-protein signaling coupled to IP3 second messenger
GO:0051899 membrane depolarization
GO:0051932 synaptic transmission, GABAergic
GO:0051966 regulation of synaptic transmission, glutamatergic
Regulatory
For research purposes only

Applications of Metabotropic Glutamate Receptor 1a antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/500
Western Blotting 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1871 detects bands of approximately 125kDa (monomer) and 250kDa (dimer) on lysate from transfected HEK293 cells.

This antibody has also been reported to detect bands at approximately 120kDa (reduced) and 210kD (non-reduced), in protein extracts from adult rat cerebellum.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

References for Metabotropic Glutamate Receptor 1a antibody

  1. Copani, A. et al. (2000) Reducing conditions differentially affect the functional and structural properties of group-I and -II metabotropic glutamate receptors.
    Brain Res. 867 (1-2): 165-72.