Metabotropic Glutamate Receptor 1a antibody
Rabbit anti Rat metabotropic glutamate receptor 1a antibody recognizes rat metabotropic glutamate receptor 1a (mGluR1a). The metabotropic glutamate receptors have been organised into three groups. mGluR5 and mGluR1 make up group I which, when activated, stimulates phospholipase C and activates protein kinase C to hydrolyse phosphoinositide phospholipids in the cell membrane. Group I mGluRs, but not other groups, are activated by 3, 5-dihydroxyphenylglycine (DHPG).
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to metabotropic glutamate receptor 1a were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- 10mM HEPES pH7.5, 150mM NaCl
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
0.01% Bovine Serum Albumin
50% Glycerol - Immunogen
- Peptide corresponding to amino acids 1115-1130 from the C-terminal region of rat mGluR1a.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P23385
- Entrez Gene
- Grm1
- GO Terms
- GO:0005515 protein binding
- GO:0001639 PLC activating metabotropic glutamate receptor activity
- GO:0004930 G-protein coupled receptor activity
- GO:0005887 integral to plasma membrane
- GO:0007206 activation of phospholipase C activity by metabotropic glutamate receptor signaling pathway
- GO:0019722 calcium-mediated signaling
- GO:0014048 regulation of glutamate secretion
- GO:0014069 postsynaptic density
- GO:0019233 sensory perception of pain
- GO:0030424 axon
- GO:0042734 presynaptic membrane
- GO:0043197 dendritic spine
- GO:0051482 elevation of cytosolic calcium ion concentration involved in G-protein signaling coupled to IP3 second messenger
- GO:0051899 membrane depolarization
- GO:0051932 synaptic transmission, GABAergic
- GO:0051966 regulation of synaptic transmission, glutamatergic
- Regulatory
- For research purposes only
Applications of Metabotropic Glutamate Receptor 1a antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | 1/500 | ||
| Western Blotting | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1871 detects bands of approximately 125kDa (monomer) and 250kDa (dimer) on lysate from transfected HEK293 cells.
This antibody has also been reported to detect bands at approximately 120kDa (reduced) and 210kD (non-reduced), in protein extracts from adult rat cerebellum.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for Metabotropic Glutamate Receptor 1a antibody
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Copani, A. et al. (2000) Reducing conditions differentially affect the functional and structural properties of group-I and -II metabotropic glutamate receptors.
Brain Res. 867 (1-2): 165-72.
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