MEK 5 (pSer311/pThr315) antibody

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Rabbit anti MEK 5 (pSer311/pThr315)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1302 WB* 0.1 ml
Rabbit anti Rat MEK 5 (pSer311/pThr315) antibody recognizes MAPK/ERK kinase 5 (MEK 5), also known as dual specificity mitogen-activated protein kinase kinase 5 (MAPKK 5), when phosphorylated at serine 311 and threonine 315.

MEK5 is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family, which acts as a key regulator in a mitogen-activated protein (MAP) kinase cascade, and hence growth factor-induced cellular stimulation. MEK5 exists in naturally occurring MEK5alpha and MEK5beta splice variants, the former of which is known to specifically activate/regulate MAPK7/ERK5/BMK1, whilst the latter may act as a suppressor of MAPK7 activation. Activation of MEK5 itself is regulated by upstream MAP3K3/MEKK3, and phosphorylation of MEK5 at both the serine 311 and threonine 315 sites has been implicated in cell proliferation.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Dog Expected from Sequence
Primate Expected from Sequence
Xenopus Expected from Sequence
Zebrafish Expected from Sequence
Human Expected from Sequence
Bovine Expected from Sequence
Chicken Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to MEK5 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
10mM HEPES pH7.5, 150mM NaCl
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
0.01%Bovine Serum Albumin
50% Glycerol
Immunogen
Synthetic phosphopeptide corresponding to an amino acid sequence within MEK5 , which includes the phoshorylated residues Ser311 and Thr315.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

UniProt
Q62862 Related reagents
Entrez Gene
Map2k5 Related reagents
GO Terms
GO:0016020 membrane
GO:0005829 cytosol
GO:0004707 MAP kinase activity
GO:0004708 MAP kinase kinase activity
GO:0004713 protein tyrosine kinase activity
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0006468 protein phosphorylation
GO:0030307 positive regulation of cell growth
GO:0050679 positive regulation of epithelial cell proliferation
GO:0070375 BMK cascade
Regulatory
For research purposes only

Applications of MEK 5 (pSer311/pThr315) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1 1/1000
  1. 1For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1302 detects a band of approximately 49kDa in rat testes cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Product Specific References

References for MEK 5 (pSer311/pThr315) antibody

  1. Cameron, S.J. et al. (2004) Differential role of MEK5alpha and MEK5beta in BMK1/ERK5 activation.
    J Biol Chem. 279 (2): 1506-12.