MEK 5 (pSer311/pThr315) antibody
Rabbit anti Rat MEK 5 (pSer311/pThr315) antibody recognizes MAPK/ERK kinase 5 (MEK 5), also known as dual specificity mitogen-activated protein kinase kinase 5 (MAPKK 5), when phosphorylated at serine 311 and threonine 315.
MEK5 is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family, which acts as a key regulator in a mitogen-activated protein (MAP) kinase cascade, and hence growth factor-induced cellular stimulation. MEK5 exists in naturally occurring MEK5alpha and MEK5beta splice variants, the former of which is known to specifically activate/regulate MAPK7/ERK5/BMK1, whilst the latter may act as a suppressor of MAPK7 activation. Activation of MEK5 itself is regulated by upstream MAP3K3/MEKK3, and phosphorylation of MEK5 at both the serine 311 and threonine 315 sites has been implicated in cell proliferation.
MEK5 is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family, which acts as a key regulator in a mitogen-activated protein (MAP) kinase cascade, and hence growth factor-induced cellular stimulation. MEK5 exists in naturally occurring MEK5alpha and MEK5beta splice variants, the former of which is known to specifically activate/regulate MAPK7/ERK5/BMK1, whilst the latter may act as a suppressor of MAPK7 activation. Activation of MEK5 itself is regulated by upstream MAP3K3/MEKK3, and phosphorylation of MEK5 at both the serine 311 and threonine 315 sites has been implicated in cell proliferation.
Product Details
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Dog Expected from Sequence Primate Expected from Sequence Xenopus Expected from Sequence Zebrafish Expected from Sequence Human Expected from Sequence Bovine Expected from Sequence Chicken Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to MEK5 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- 10mM HEPES pH7.5, 150mM NaCl
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) 0.01% Bovine Serum Albumin 50% Glycerol - Immunogen
- Synthetic phosphopeptide corresponding to an amino acid sequence within MEK5 , which includes the phoshorylated residues Ser311 and Thr315.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of despatch.
More Information
- UniProt
- Q62862 Related reagents
- Entrez Gene
- Map2k5 Related reagents
- GO Terms
- GO:0016020 membrane
- GO:0005829 cytosol
- GO:0004707 MAP kinase activity
- GO:0004708 MAP kinase kinase activity
- GO:0004713 protein tyrosine kinase activity
- GO:0046872 metal ion binding
- GO:0005524 ATP binding
- GO:0005634 nucleus
- GO:0006468 protein phosphorylation
- GO:0030307 positive regulation of cell growth
- GO:0050679 positive regulation of epithelial cell proliferation
- GO:0070375 BMK cascade
- Regulatory
- For research purposes only
Applications of MEK 5 (pSer311/pThr315) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting 1 | 1/1000 |
- 1For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1302 detects a band of approximately 49kDa in rat testes cell lysates.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Product Specific References
References for MEK 5 (pSer311/pThr315) antibody
-
Cameron, S.J. et al. (2004) Differential role of MEK5alpha and MEK5beta in BMK1/ERK5 activation.
J Biol Chem. 279 (2): 1506-12.
