Rabbit anti Rat MCP-1 antibodyrecognizes rat monocyte chemo attractant protein-1 (MCP-1), also known as CCL-2.
Rat MCP-1 is a chemokine of approximately 12 kDa, which plays an important role in the inflammatory response of blood monocytes and tissue macrophages.
- Target Species
- Product Form
- Purified IgG - lyophilized
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
- Antiserum Preparation
- Antisera to rat MCP-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Recombinant rat MCP-1.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution.
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
response to hypoxia
positive regulation of endothelial cell proliferation
cellular calcium ion homeostasis
response to mechanical stimulus
transforming growth factor beta receptor signaling pathway
response to drug
response to heat
response to gamma radiation
response to activity
response to antibiotic
CCR2 chemokine receptor binding
response to lipopolysaccharide
response to progesterone stimulus
response to vitamin B3
neuronal cell body
response to amino acid stimulus
response to ethanol
vascular endothelial growth factor receptor signaling pathway
positive regulation of synaptic transmission
response to glucocorticoid stimulus
maternal process involved in parturition
chemokine-mediated signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of MCP-1 antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems with the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
References for MCP-1 antibody
Zhang, H. et al. (2016) Dorsal root ganglion infiltration by macrophages contributes to paclitaxel chemotherapy induced peripheral neuropathy.
J Pain. Mar 12. pii: S1526-5900(16)00562-9. [Epub ahead of print]
Aharon-Hananel, G. et al. (2015) Antidiabetic Effect of Interleukin-1β Antibody Therapy Through β-Cell Protection in the Cohen Diabetes-Sensitive Rat.
Diabetes. 64 (5): 1780-5.
Schreiber, R.C. et al. (2001) Monocyte chemoattractant protein (MCP)-1 is rapidly expressed by sympathetic ganglion neurons following axonal injury.
Neuroreport. 12 (3): 601-6.
Tomas-sanchez, C. et al. (2018) Prophylactic Zinc and Therapeutic Selenium Administration Increases the Antioxidant Enzyme Activity in the Rat Temporoparietal Cortex and Improves Memory after a Transient Hypoxia-Ischemia.
Oxid Med Cell Longev. 2018: 9416432.
Rong, Y. et al. (2021) Small extracellular vesicles encapsulating CCL2 from activated astrocytes induce microglial activation and neuronal apoptosis after traumatic spinal cord injury.
J Neuroinflammation. 18 (1): 196.
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