MAPK8/MAPK9 (pThr183/pTyr185)/MAPK10 (pThr221/pTyr223) antibody

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Rabbit anti MAPK8/MAPK9 (pThr183/pTyr185)/MAPK10 (pThr221/pTyr223)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2693 WB 50 µl
Rabbit anti MAPK8/MAPK9 (pThr183/pTyr185)/MAPK10 (pThr221/pTyr223) antibody recognizes mitogen activated protein kinase (MAPK) 8 and 9, also known as c-Jun N-terminal kinases 1 and 2 or stress activated protein kinases 1 and 2, when phosphorylated on threonine 183 and tyrosine 185. This antibody also recognizes MAPK10, also known as c-Jun N-terminal kinase 3 or stress activated protein kinase 3, when phosphorylated on threonine 221 and tyrosine 223.

As their name implies, MAPK8/9/10 are members of the serine/threonine MAP kinase family, which become activated by environmental stressors. In addition, activation through phosphorylation events has been reported upon stimulation of toll-like receptors and exposure to pro-inflammatory cytokines (Guma and Firestein 2012, Bogoyevitch and Kobe 2006).

Two phosphorylation events are critical for the activation of the three MAPKs. The first is on threonine 183, or in the case of MAPK10 threonine 221, by dual specificity mitogen-activated kinase kinase 7 (MKK7) and the second is on tyrosine 185, or in the case of MAPK10 tyrosine 223, by MKK4 (Fleming 2000). The phosphorylation undertaken by MKK4 and MKK7 is counteracted by phosphatases of the MAP kinase phosphatase (MKP) family (Guma and Firestein 2012).

The three MAPKs play a critical role in mediating cell signaling cascades by phosphorylating transcription factors, such as c-Jun and p53, thereby regulating cellular processes including proliferation and apoptosis (Guma and Firestein 2012).

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human Expected from Sequence
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to MAPK8/MAPK9 (pThr183/pTyr185)/MAPK10 (pThr221/pTyr223) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding threonine 183/tyrosine 185 of human MAPK8/MAPK9 and to residues surrounding threonine 221/tyrosine 223 of human MAPK10.

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P45983 Related reagents
P45984 Related reagents
P53779 Related reagents
Entrez Gene
MAPK8 Related reagents
MAPK9 Related reagents
MAPK10 Related reagents
GO Terms
GO:0005515 protein binding
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0005829 cytosol
GO:0004705 JUN kinase activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0008063 Toll signaling pathway
GO:0008629 induction of apoptosis by intracellular signals
GO:0008633 activation of pro-apoptotic gene products
GO:0009411 response to UV
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0043066 negative regulation of apoptosis
GO:0045087 innate immune response
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051090 regulation of transcription factor activity
GO:0051403 stress-activated MAPK cascade
GO:0004708 MAP kinase kinase activity
Regulatory
For research purposes only

Applications of MAPK8/MAPK9 (pThr183/pTyr185)/MAPK10 (pThr221/pTyr223) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Guma M & Firestein GS (2012) c-Jun N-Terminal Kinase in Inflammation and Rheumatic Diseases.
    Open Rheumatol J. 6: 220-31.
  2. Bogoyevitch MA & Kobe B (2006) Uses for JNK: the many and varied substrates of the c-Jun N-terminal kinases.
    Microbiol Mol Biol Rev. 70 (4): 1061-95.
  3. Fleming Y et al. (2000) Synergistic activation of stress-activated protein kinase 1/c-Jun N-terminal kinase (SAPK1/JNK) isoforms by mitogen-activated protein kinase kinase 4 (MKK4) and MKK7.
    Biochem J. 352 Pt 1: 145-54.