Leptin antibody
Goat anti Rat leptin antibody (AAR40) used to immunostain leptin expressing tissues by immunofluorescence on rabbit mammary gland sections.
Image caption:
Leptin expression and localisation in mammary glands from C and DE rabbits at day 28 of pregnancy.
(A) Expression of leptin in mammary glands at day 28 of pregnancy from control (in light grey) and exposed (DE) (in black) dams. Real-time RT-PCR was quantified using TBP housekeeping gene. Data are mean ± SEM. (B and C) Representative images of leptin immunostaining (red) of mammary gland from control (B) or exposed (C) dams at day 28 of pregnancy. Nuclei are stained in green. Scale bar represents 50μm.
From: Hue-Beauvais C, Aujean E, Miranda G, Ralliard-Rousseau D, Valentino S, Brun N, et al. (2019)
Impact of exposure to diesel exhaust during pregnancy on mammary gland development and milk composition in the rabbit.
PLoS ONE 14(2): e0212132.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Goat anti Rat Leptin antibody specifically recognizes rat leptin, a major adipose-derived hormone and member of the leptin family, which plays a key role in appetite control and the regulation of energy expenditure.
Leptin is a recognized marker of obesity, which signals through binding to the obesity receptor (OB-R) in the hypothalamus. Human studies have shown a correlation between circulating levels of leptin and body fat mass, and defects in leptin production result in severe obesity. Regulators of leptin expression include insulin, cortisol, cAMP and thiazolidinediones.
Leptin is a recognized marker of obesity, which signals through binding to the obesity receptor (OB-R) in the hypothalamus. Human studies have shown a correlation between circulating levels of leptin and body fat mass, and defects in leptin production result in severe obesity. Regulators of leptin expression include insulin, cortisol, cAMP and thiazolidinediones.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antiserum to rat Leptin were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant rat leptin.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at +-20oC .
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of reconstitution.
More Information
- UniProt
- P50596 Related reagents
- Entrez Gene
- Lep Related reagents
- GO Terms
- GO:0001542 ovulation from ovarian follicle
- GO:0005125 cytokine activity
- GO:0005179 hormone activity
- GO:0005615 extracellular space
- GO:0006112 energy reserve metabolic process
- GO:0007259 JAK-STAT cascade
- GO:0008343 adult feeding behavior
- GO:0009062 fatty acid catabolic process
- GO:0009892 negative regulation of metabolic process
- GO:0019933 cAMP-mediated signaling
- GO:0032099 negative regulation of appetite
- GO:0033210 leptin-mediated signaling pathway
- GO:0043410 positive regulation of MAPKKK cascade
- GO:0051428 peptide hormone receptor binding
- Regulatory
- For research purposes only
Applications of Leptin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.5 | 2.0ug/ml | |
| Western Blotting | 0.1 | 0.2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | 1 mg | F | ||
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | 1 mg | C E WB |
Product Specific References
References for Leptin antibody
-
Hue-beauvais, C. et al. (2019) Impact of exposure to diesel exhaust during pregnancy on mammary gland development and milk composition in the rabbit.
PLoS One. 14 (2): e0212132.
