Leptin antibody
Leptin is a recognized marker of obesity, which signals through binding to the obesity receptor (OB-R) in the hypothalamus. Human studies have shown a correlation between circulating levels of leptin and body fat mass, and defects in leptin production result in severe obesity. Regulators of leptin expression include insulin, cortisol, cAMP and thiazolidinediones.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antiserum to rat Leptin were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant rat leptin.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution
Storage Information
- Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P50596
- Entrez Gene
- Lep
- GO Terms
- GO:0001542 ovulation from ovarian follicle
- GO:0005125 cytokine activity
- GO:0005179 hormone activity
- GO:0005615 extracellular space
- GO:0006112 energy reserve metabolic process
- GO:0007259 JAK-STAT cascade
- GO:0008343 adult feeding behavior
- GO:0009062 fatty acid catabolic process
- GO:0009892 negative regulation of metabolic process
- GO:0019933 cAMP-mediated signaling
- GO:0032099 negative regulation of appetite
- GO:0033210 leptin-mediated signaling pathway
- GO:0043410 positive regulation of MAPKKK cascade
- GO:0051428 peptide hormone receptor binding
- Regulatory
- For research purposes only
Applications of Leptin antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 0.5 | 2.0ug/ml | |
Western Blotting | 0.1 | 0.2ug/ml |
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
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Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
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Product Specific References
References for Leptin antibody
-
Hue-beauvais, C. et al. (2019) Impact of exposure to diesel exhaust during pregnancy on mammary gland development and milk composition in the rabbit.
PLoS One. 14 (2): e0212132.
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