Leptin antibody

Goat anti Rat Leptin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Leptin

Product Code Applications Pack Size List Price Your Price Qty
AAR40
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SDS Safety Datasheet SDS
E WB 0.1 mg loader
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Goat anti Rat Leptin antibody specifically recognizes rat leptin, a major adipose-derived hormone and member of the leptin family, which plays a key role in appetite control and the regulation of energy expenditure.

Leptin is a recognized marker of obesity, which signals through binding to the obesity receptor (OB-R) in the hypothalamus. In humans there is a correlation between circulating levels of leptin and body fat mass, and defects in leptin production result in severe obesity. Regulators of leptin expression include insulin, cortisol, cAMP and thiazolidinediones.

Target Species
Rat
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antiserum to rat Leptin were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant rat leptin.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Western Blotting 0.1 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for Leptin antibody

  1. Hue-beauvais, C. et al. (2019) Impact of exposure to diesel exhaust during pregnancy on mammary gland development and milk composition in the rabbit.
    PLoS One. 14 (2): e0212132.

RRID
AB_2136211
UniProt
P50596
Entrez Gene
Lep
GO Terms
GO:0001542 ovulation from ovarian follicle
GO:0005125 cytokine activity
GO:0005179 hormone activity
GO:0005615 extracellular space
GO:0006112 energy reserve metabolic process
GO:0007259 JAK-STAT cascade
GO:0008343 adult feeding behavior
GO:0009062 fatty acid catabolic process
GO:0009892 negative regulation of metabolic process
GO:0019933 cAMP-mediated signaling
GO:0032099 negative regulation of appetite
GO:0033210 leptin-mediated signaling pathway
GO:0043410 positive regulation of MAPKKK cascade
GO:0051428 peptide hormone receptor binding
View more products with LEPTIN specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Rat Products