Leptin antibody

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Goat anti Rat Leptin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAR40 E WB 0.1 mg
Goat anti Rat Leptin antibody specifically recognizes rat leptin, a major adipose-derived hormone and member of the leptin family, which plays a key role in appetite control and the regulation of energy expenditure.

Leptin is a recognized marker of obesity, which signals through binding to the obesity receptor (OB-R) in the hypothalamus. Human studies have shown a correlation between circulating levels of leptin and body fat mass, and defects in leptin production result in severe obesity. Regulators of leptin expression include insulin, cortisol, cAMP and thiazolidinediones.

Product Details

Target Species
Rat
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antiserum to rat Leptin were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant rat leptin.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +-20oC .
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P50596 Related reagents
Entrez Gene
Lep Related reagents
GO Terms
GO:0001542 ovulation from ovarian follicle
GO:0005125 cytokine activity
GO:0005179 hormone activity
GO:0005615 extracellular space
GO:0006112 energy reserve metabolic process
GO:0007259 JAK-STAT cascade
GO:0008343 adult feeding behavior
GO:0009062 fatty acid catabolic process
GO:0009892 negative regulation of metabolic process
GO:0019933 cAMP-mediated signaling
GO:0032099 negative regulation of appetite
GO:0033210 leptin-mediated signaling pathway
GO:0043410 positive regulation of MAPKKK cascade
GO:0051428 peptide hormone receptor binding
Regulatory
For research purposes only

Applications of Leptin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Western Blotting 0.1 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB