IL-6 antibody

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Rabbit anti Rat Interleukin-6:Biotin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAR17B E WB 50 µg
Rabbit anti Rat Interleukin-6 antibody recognizes rat interleukin-6.

IL-6 is a pleiotropic cytokine involved in the regulation of immune and inflammatory responses.

Product Details

Target Species
Rat
Product Form
Purified IgG conjugated to Biotin - lyophilised
Reconstitution
Reconstitute with 0.5 ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antisera to rat IL-6 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None Present
Immunogen
Recombinant Rat IL-6 (PRP21)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P20607 Related reagents
Entrez Gene
Il6 Related reagents
GO Terms
GO:0006953 acute-phase response
GO:0005125 cytokine activity
GO:0005138 interleukin-6 receptor binding
GO:0005615 extracellular space
GO:0045454 cell redox homeostasis
GO:0006469 negative regulation of protein kinase activity
GO:0009612 response to mechanical stimulus
GO:0006955 immune response
GO:0007568 aging
GO:0008083 growth factor activity
GO:0008285 negative regulation of cell proliferation
GO:0008360 regulation of cell shape
GO:0042493 response to drug
GO:0009408 response to heat
GO:0009409 response to cold
GO:0046677 response to antibiotic
GO:0031000 response to caffeine
GO:0031667 response to nutrient levels
GO:0032496 response to lipopolysaccharide
GO:0032868 response to insulin stimulus
GO:0033160 positive regulation of protein import into nucleus, translocation
GO:0034097 response to cytokine stimulus
GO:0043154 negative regulation of caspase activity
GO:0043200 response to amino acid stimulus
GO:0043526 neuroprotection
GO:0045188 regulation of circadian sleep/wake cycle, non-REM sleep
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0045666 positive regulation of neuron differentiation
GO:0045721 negative regulation of gluconeogenesis
GO:0045740 positive regulation of DNA replication
GO:0046427 positive regulation of JAK-STAT cascade
GO:0046849 bone remodeling
GO:0048635 negative regulation of muscle organ development
GO:0050710 negative regulation of cytokine secretion
GO:0051592 response to calcium ion
GO:0051602 response to electrical stimulus
GO:0051789 response to protein stimulus
GO:0051971 positive regulation of transmission of nerve impulse
GO:0070374 positive regulation of ERK1 and ERK2 cascade
Regulatory
For research purposes only

Applications of IL-6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Western Blotting 0.1ug/ml 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
Anti Rat IL-6: biotin conjugate may be used in a direct ELISA with recombinant rat IL-6 (PRP21) as the standard.