IL-6 antibody

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Rabbit anti Rat Interleukin-6:Biotin

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
AAR17B Datasheet	E WB SDS	50 µg	Log in		Get Quote

Summary
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Rabbit anti Rat Interleukin-6 antibody recognizes rat interleukin-6.

IL-6 is a pleiotropic cytokine involved in the regulation of immune and inflammatory responses.

Product Details

Target Species: Rat
Product Form: Purified IgG conjugated to Biotin - lyophilised
Reconstitution: Reconstitute with 0.5 ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation: Antisera to rat IL-6 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: None Present
Carrier Free: Yes
Immunogen: Recombinant Rat IL-6 (PRP21)
Approx. Protein Concentrations: IgG concentration 0.1 mg/ml after reconstitution

Storage Information

Storage: Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 12 months from date of despatch

More Information

UniProt: P20607
Entrez Gene: Il6
GO Terms: GO:0006953 acute-phase response; GO:0005125 cytokine activity; GO:0005138 interleukin-6 receptor binding; GO:0005615 extracellular space; GO:0045454 cell redox homeostasis; GO:0006469 negative regulation of protein kinase activity; GO:0009612 response to mechanical stimulus; GO:0006955 immune response; GO:0007568 aging; GO:0008083 growth factor activity; GO:0008285 negative regulation of cell proliferation; GO:0008360 regulation of cell shape; GO:0042493 response to drug; GO:0009408 response to heat; GO:0009409 response to cold; GO:0046677 response to antibiotic; GO:0031000 response to caffeine; GO:0031667 response to nutrient levels; GO:0032496 response to lipopolysaccharide; GO:0032868 response to insulin stimulus; GO:0033160 positive regulation of protein import into nucleus, translocation; GO:0034097 response to cytokine stimulus; GO:0043154 negative regulation of caspase activity; GO:0043200 response to amino acid stimulus; GO:0043526 neuroprotection; GO:0045188 regulation of circadian sleep/wake cycle, non-REM sleep; GO:0045429 positive regulation of nitric oxide biosynthetic process; GO:0045666 positive regulation of neuron differentiation; GO:0045721 negative regulation of gluconeogenesis; GO:0045740 positive regulation of DNA replication; GO:0046427 positive regulation of JAK-STAT cascade; GO:0046849 bone remodeling; GO:0048635 negative regulation of muscle organ development; GO:0050710 negative regulation of cytokine secretion; GO:0051592 response to calcium ion; GO:0051602 response to electrical stimulus; GO:0051789 response to protein stimulus; GO:0051971 positive regulation of transmission of nerve impulse; GO:0070374 positive regulation of ERK1 and ERK2 cascade
Regulatory: For research purposes only

Applications of IL-6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1.0 ug/ml
Western Blotting		0.1ug/ml	0.2ug/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

ELISA: Anti Rat IL-6: biotin conjugate may be used in a direct ELISA with recombinant rat IL-6 (PRP21) as the standard.

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