Rabbit anti Rat Interleukin-2 antibody specifically recognizes rat interleukin-2 (IL-2), otherwise known as T-cell growth factor, a multi-functional immunoregulatory cytokine and member of the IL-2 family, secreted by antigen or mitogen stimulated T cells.
IL-2 signals through the IL-2 receptor complex (IL-2R), and is principally involved in the stimulation of the T cell proliferative response, promoting T cell clonal expansion and the expansion of T memory cells expressing low levels of high-affinity IL-2R. IL-2 also stimulates the growth/differentiation of B cells, monocytes, NK cells, macrophages and lymphokine activated killer cells.
- Target Species
- Product Form
- Purified IgG - lyophilised
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
- Antiserum Preparation
- Antiserum to rat IL-2 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Recombinant rat IL-2.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
negative regulation of protein phosphorylation
interleukin-2 receptor binding
elevation of cytosolic calcium ion concentration
activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway
growth factor activity
kappa-type opioid receptor binding
positive regulation of regulatory T cell differentiation
negative regulation of heart contraction
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IL-2 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available