IL-1 Alpha antibody
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Goat anti Rat Interleukin-1 alpha antibody recognizes rat interleukin-1 alpha. Rat IL-1 alpha is a 17.7kD cytokine, which is produced by macrophages.
IL-1 alpha is chemotactic for leucocytes and also contributes to the inflammatory response by its interactions with several other cell types.
IL-1 alpha is chemotactic for leucocytes and also contributes to the inflammatory response by its interactions with several other cell types.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Biotin - lyophilised
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.5 ml sterile PBS containing 0.1% Bovine Serum Albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Reconstitution
- Reconstitute with 0.1 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Antiserum Preparation
- Antisera to rat IL-1 alpha were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None Present
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant rat IL-1 alpha.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml after reconstitution
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. The addition of 0.09% sodium azide is recommended for long term storage. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. The addition of 0.09% sodium azide is recommended for long term storage.
N.B. For functional studies do not add sodium azide. - Guarantee
- 12 months from date of reconstitution.
- Guarantee
- 12 months from date of reconstitution.
More Information
- UniProt
- P16598 Related reagents
- Entrez Gene
- Il1a Related reagents
- GO Terms
- GO:0001660 fever generation
- GO:0001666 response to hypoxia
- GO:0005829 cytosol
- GO:0005125 cytokine activity
- GO:0005150 interleukin-1, Type I receptor binding
- GO:0005507 copper ion binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0009986 cell surface
- GO:0010193 response to ozone
- GO:0010243 response to organic nitrogen
- GO:0010332 response to gamma radiation
- GO:0010893 positive regulation of steroid biosynthetic process
- GO:0014070 response to organic cyclic compound
- GO:0031424 keratinization
- GO:0032496 response to lipopolysaccharide
- GO:0032755 positive regulation of interleukin-6 production
- GO:0032874 positive regulation of stress-activated MAPK cascade
- GO:0033591 response to L-ascorbic acid
- GO:0034605 cellular response to heat
- GO:0042060 wound healing
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0046688 response to copper ion
- GO:0046330 positive regulation of JNK cascade
- GO:0051781 positive regulation of cell division
- GO:0051930 regulation of sensory perception of pain
- GO:0070374 positive regulation of ERK1 and ERK2 cascade
- Regulatory
- For research purposes only
Applications of IL-1 Alpha antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.15ug/ml | 0.30ug/ml | |
| Western Blotting | 0.1ug/ml | 0.2ug/ml | |
| ELISA | 0.5ug/ml | ||
| Functional Assays | 0.011ug/ml | 0.017ug/ml | |
| Western Blotting | 0.1ug/ml | 0.2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | 1 mg | F | ||
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | 1 mg | C E WB |
