IL-1 Alpha antibody

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Goat anti Rat Interleukin-1 Alpha:Biotin

Goat anti Rat Interleukin-1 Alpha

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAR25B E WB 50 µg
AAR25 E FN WB 0.1 mg
Goat anti Rat Interleukin-1 alpha antibody recognizes rat interleukin-1 alpha. Rat IL-1 alpha is a 17.7kD cytokine, which is produced by macrophages.

IL-1 alpha is chemotactic for leucocytes and also contributes to the inflammatory response by its interactions with several other cell types.

Product Details

Target Species
Rat
Product Form
Purified IgG conjugated to Biotin - lyophilised
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 1.0 ml sterile PBS containing 0.1% Bovine Serum Albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Reconstitution
Reconstitute with 0.1 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Antiserum Preparation
Antisera to rat IL-1 alpha were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None Present
Preservative Stabilisers
None present
Immunogen
Recombinant rat IL-1 alpha.
Approx. Protein Concentrations
IgG concentration 50 ug/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. The addition of 0.09% sodium azide is recommended for long term storage.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. The addition of 0.09% sodium azide is recommended for long term storage.

N.B. For functional studies do not add sodium azide.
Shelf Life
12 months from date of reconstitution.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P16598 Related reagents
Entrez Gene
Il1a Related reagents
GO Terms
GO:0001660 fever generation
GO:0001666 response to hypoxia
GO:0005829 cytosol
GO:0005125 cytokine activity
GO:0005150 interleukin-1, Type I receptor binding
GO:0005507 copper ion binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0009986 cell surface
GO:0010193 response to ozone
GO:0010243 response to organic nitrogen
GO:0010332 response to gamma radiation
GO:0010893 positive regulation of steroid biosynthetic process
GO:0014070 response to organic cyclic compound
GO:0031424 keratinization
GO:0032496 response to lipopolysaccharide
GO:0032755 positive regulation of interleukin-6 production
GO:0032874 positive regulation of stress-activated MAPK cascade
GO:0033591 response to L-ascorbic acid
GO:0034605 cellular response to heat
GO:0042060 wound healing
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0046688 response to copper ion
GO:0046330 positive regulation of JNK cascade
GO:0051781 positive regulation of cell division
GO:0051930 regulation of sensory perception of pain
GO:0070374 positive regulation of ERK1 and ERK2 cascade
Regulatory
For research purposes only

Applications of IL-1 Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.15ug/ml 0.30ug/ml
Western Blotting 0.1ug/ml 0.2ug/ml
ELISA 0.5ug/ml
Functional Assays 0.011ug/ml 0.017ug/ml
Western Blotting 0.1ug/ml 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB