IgG antibody
FITC conjugated Donkey anti Rat IgG antibody used to detect bound Rat anti Mouse CD31 antibody in a choroidal neovascularization study using immunofluorescence microscopy.
Image caption:
Choroidal neovascularization (CNV) detection by CD31 immunofluorescence. Mice that received only the vehicle (upper left, group V) had larger areas of CNV than mice that received the supplement (upper right) and/or anti-vascular endothelial growth factor (VEGF) treatment. Treatment groups included the nutritional supplement (S) group, the supplement and anti-vascular endothelial growth factor agent (S + aVEGF) group, and the vehicle and anti-VEGF agent (V + aVEGF) group.
From: Ivanescu AA, Fernández-Robredo P, Heras-Mulero H, Sádaba-Echarri LM, García-García L, Fernández-García V, Moreno-Orduna M, Redondo-Exposito A, Recalde S, García-Layana A.
Modifying Choroidal Neovascularization Development with a Nutritional Supplement in Mice.
Nutrients. 2015 Jul 6;7(7):5423-42.
Donkey anti Rat IgG (H/L):Alk. Phos. (Mouse Adsorbed)
Donkey anti Rat IgG (H/L):Biotin (Mouse Adsorbed)
Donkey anti Rat IgG (H/L):FITC (Mouse Adsorbed)
Donkey anti Rat IgG (H/L):HRP (Mouse Adsorbed)
Donkey anti Rat IgG (H/L) (Mouse Adsorbed)
Donkey anti Rat IgG (H/L):RPE (Mouse Adsorbed)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| 643004 | C E WB | 1 ml |
|
|
| 643008 | C E | 1 mg |
|
|
| 643002 | IF | 1 mg |
|
|
| 643005 | C E | 1 ml |
|
|
| 643001 | C E | 1 mg |
|
|
| 643009 | F | 0.5 mg |
|
Donkey anti Rat IgG antibody recognizes rat IgG heavy and light chains and demonstrates minimal cross reactivity with mouse serum proteins.
Product Details
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alkaline Phosphatase - liquid
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Ig Fraction conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Ig Fraction - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Rat IgG covalently linked to agarose.
- Buffer Solution
- TRIS buffered saline, 1mM MgCl2
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Borate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
50% Glycerol - Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Preservative Stabilisers
- 50% Glycerol
- Preservative Stabilisers
- None present
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
Stabilizing agent (sucrose) - Immunogen
- Rat IgG.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- 1.0 mg/ml
- Approx. Protein Concentrations
- 1.0 mg/ml
- Approx. Protein Concentrations
- 0.5 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- -20ºC only (ship +4ºC)
- Storage
- -20ºC only (ship +4ºC)
- Storage
- +4ºC DO NOT FREEZE
This product is photosensitive and should be protected from light. - Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- UniProt
- P20767
- P20760
- P20759
- P20761
- P20762
- P01835
- P01836
- P20766
- Entrez Gene
- LOC500180
- Ighg
- LOC679045
- LOC362795
- RGD1564318
- GO Terms
- GO:0003823 antigen binding
- Regulatory
- For research purposes only
Applications of IgG antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/2000 | 1/4000 | |
| Immunohistology - Frozen | |||
| Western Blotting | |||
| ELISA | |||
| Immunohistology - Frozen | |||
| Immunofluorescence | |||
| ELISA | |||
| Immunohistology - Frozen | |||
| ELISA | |||
| Immunohistology - Frozen | |||
| Flow Cytometry | 1/10 | 1/50 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for IgG antibody
-
Bombardieri, M. et al. (2011) A BAFF/APRIL-dependent TLR3-stimulated pathway enhances the capacity of rheumatoid synovial fibroblasts to induce AID expression and Ig class-switching in B cells.
Ann Rheum Dis. 70 (10): 1857-65. -
Ivanescu, A.A. et al. (2015) Modifying Choroidal Neovascularization Development with a Nutritional Supplement in Mice.
Nutrients. 7 (7): 5423-42.
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