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ERK / MAPK (pThr202/pTyr204) antibody

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Anti ERK / MAPK (pThr202/pTyr204) Antibody gallery image 1
Western blot of human T47D cells showing phospho-specific immunolabeling of the ~42-44 kDa ERK/MAPK protein doublet

Rabbit anti ERK / MAPK (pThr202/pTyr204)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP901 WB* 0.1 ml
Rabbit anti Rat ERK/MAPK (pThr202/pTyr204) antibody recognizes extracellular regulated kinase (ERK), also known as MAPK, when phosphorylated at threonine 202 and tyrosine 204.

ERK/MAPK is an important integration point of many signalling pathways, for a range of cellular processes including proliferation, differentiation, transcription regulation and development. Upon phosphorylation, activated ERK/MAPK translocates to the nucleus, where it phosphorylates nuclear targets.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Bovine Expected from Sequence
Chicken Expected from Sequence
Monkey Expected from Sequence
Mouse Expected from Sequence
Dog Expected from Sequence
Xenopus Expected from Sequence
Zebrafish Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to phosphorylated rat ERK/MAPK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
10mM HEPES pH7.5, 150mM NaCl
Preservative Stabilisers
0.05%Sodium Azide
0.01%Bovine Serum Albumin
50%Glycerol
Immunogen
Synthetic phosphopeptide corresponding to an amino acid sequence within rat ERK/MAPK which includes phosphorylated Thr202 and Tyr204.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

UniProt
P21708 Related reagents
P63086 Related reagents
Entrez Gene
Mapk3 Related reagents
Mapk1 Related reagents
GO Terms
GO:0000189 MAPK import into nucleus
GO:0005515 protein binding
GO:0005829 cytosol
GO:0004707 MAP kinase activity
GO:0005524 ATP binding
GO:0005625 soluble fraction
GO:0005626 insoluble fraction
GO:0005654 nucleoplasm
GO:0006461 protein complex assembly
GO:0006468 protein phosphorylation
GO:0007049 cell cycle
GO:0009636 response to toxin
GO:0045727 positive regulation of translation
GO:0019369 arachidonic acid metabolic process
GO:0043234 protein complex
GO:0045941 positive regulation of transcription
GO:0008134 transcription factor binding
GO:0008284 positive regulation of cell proliferation
GO:0019233 sensory perception of pain
GO:0030335 positive regulation of cell migration
GO:0031435 mitogen-activated protein kinase kinase kinase binding
GO:0032839 dendrite cytoplasm
GO:0033267 axon part
GO:0043204 perikaryon
GO:0043627 response to estrogen stimulus
Regulatory
For research purposes only

Applications of ERK / MAPK (pThr202/pTyr204) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1 1/1000
  1. 1 For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP901 recognises a band of approximately 43kD in human T47D lysates.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB
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