ERK / MAPK (pThr202/pTyr204) antibody
Rabbit anti Rat ERK/MAPK (pThr202/pTyr204) antibody recognizes extracellular regulated kinase (ERK), also known as MAPK, when phosphorylated at threonine 202 and tyrosine 204.
ERK/MAPK is an important integration point of many signaling pathways, for a range of cellular processes including proliferation, differentiation, transcription regulation and development. Upon phosphorylation, activated ERK/MAPK translocates to the nucleus, where it phosphorylates nuclear targets.
ERK/MAPK is an important integration point of many signaling pathways, for a range of cellular processes including proliferation, differentiation, transcription regulation and development. Upon phosphorylation, activated ERK/MAPK translocates to the nucleus, where it phosphorylates nuclear targets.
Product Details
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Bovine Expected from Sequence Chicken Expected from Sequence Monkey Expected from Sequence Mouse Expected from Sequence Dog Expected from Sequence Xenopus Expected from Sequence Zebrafish Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to phosphorylated rat ERK/MAPK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- 10mM HEPES pH7.5, 150mM NaCl
- Preservative Stabilisers
0.05% Sodium Azide 0.01% Bovine Serum Albumin 50% Glycerol - Immunogen
- Synthetic phosphopeptide corresponding to an amino acid sequence within rat ERK/MAPK which includes phosphorylated Thr202 and Tyr204.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P21708
- P63086
- Entrez Gene
- Mapk3
- Mapk1
- GO Terms
- GO:0000189 MAPK import into nucleus
- GO:0005515 protein binding
- GO:0005829 cytosol
- GO:0004707 MAP kinase activity
- GO:0005524 ATP binding
- GO:0005625 soluble fraction
- GO:0005626 insoluble fraction
- GO:0005654 nucleoplasm
- GO:0006461 protein complex assembly
- GO:0006468 protein phosphorylation
- GO:0007049 cell cycle
- GO:0009636 response to toxin
- GO:0045727 positive regulation of translation
- GO:0019369 arachidonic acid metabolic process
- GO:0043234 protein complex
- GO:0045941 positive regulation of transcription
- GO:0008134 transcription factor binding
- GO:0008284 positive regulation of cell proliferation
- GO:0019233 sensory perception of pain
- GO:0030335 positive regulation of cell migration
- GO:0031435 mitogen-activated protein kinase kinase kinase binding
- GO:0032839 dendrite cytoplasm
- GO:0033267 axon part
- GO:0043204 perikaryon
- GO:0043627 response to estrogen stimulus
- Regulatory
- For research purposes only
Applications of ERK / MAPK (pThr202/pTyr204) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP901 recognises a band of approximately 43kD in human T47D lysates.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
|
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
