ERK / MAPK (pThr202/pTyr204) antibody

Rabbit anti ERK / MAPK (pThr202/pTyr204)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
ERK / MAPK (pThr202/pTyr204)

Product Code Applications Pack Size List Price Your Price Qty
AHP901
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 ml loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rabbit anti Rat ERK/MAPK (pThr202/pTyr204) antibody recognizes extracellular regulated kinase (ERK), also known as MAPK, when phosphorylated at threonine 202 and tyrosine 204.

ERK/MAPK is an important integration point of many signaling pathways, for a range of cellular processes including proliferation, differentiation, transcription regulation and development. Upon phosphorylation, activated ERK/MAPK translocates to the nucleus, where it phosphorylates nuclear targets.

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Bovine Expected from Sequence
Chicken Expected from Sequence
Monkey Expected from Sequence
Mouse Expected from Sequence
Dog Expected from Sequence
Xenopus Expected from Sequence
Zebrafish Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to phosphorylated rat ERK/MAPK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
10mM HEPES pH7.5, 150mM NaCl
Preservative Stabilisers
0.05%Sodium Azide
0.01%Bovine Serum Albumin
50%Glycerol
Immunogen
Synthetic phosphopeptide corresponding to an amino acid sequence within rat ERK/MAPK which includes phosphorylated Thr202 and Tyr204.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP901 recognises a band of approximately 43kD in human T47D lysates.

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RRID
AB_566958
UniProt
P21708
P63086
Entrez Gene
Mapk3
Mapk1
GO Terms
GO:0000189 MAPK import into nucleus
GO:0005515 protein binding
GO:0005829 cytosol
GO:0004707 MAP kinase activity
GO:0005524 ATP binding
GO:0005625 soluble fraction
GO:0005626 insoluble fraction
GO:0005654 nucleoplasm
GO:0006461 protein complex assembly
GO:0006468 protein phosphorylation
GO:0007049 cell cycle
GO:0009636 response to toxin
GO:0045727 positive regulation of translation
GO:0019369 arachidonic acid metabolic process
GO:0043234 protein complex
GO:0045941 positive regulation of transcription
GO:0008134 transcription factor binding
GO:0008284 positive regulation of cell proliferation
GO:0019233 sensory perception of pain
GO:0030335 positive regulation of cell migration
GO:0031435 mitogen-activated protein kinase kinase kinase binding
GO:0032839 dendrite cytoplasm
GO:0033267 axon part
GO:0043204 perikaryon
GO:0043627 response to estrogen stimulus
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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