EGF antibody

Rabbit anti Rat EGF
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- EGF
Rabbit anti Rat EGF antibody recognizes rat EGF (Epidermal Growth Factor), a type I transmembrane protein expressed by a variety of cells, which acts as a potent stimulator and regulator of the proliferation of epidermal and epithelial cells, and is a key factor in cell survival. The effects of EGF are initiated through tyrosine kinase activity, following the binding of EGF to the extracellular domain of the EGF receptor (EGFR), which results in the triggering of several signal transduction pathways, including the JAK/STAT and P13K/AKT pathways. |
- Target Species
- Rat
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antiserum to rat EGF was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant rat EGF.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | ![]() |
0.5 | 2.0ug/ml |
Functional Assays | ![]() |
0.05 | 0.1ug/ml |
Western Blotting | ![]() |
0.5 | 1.0ug/ml |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Description | Sheep anti Rabbit IgG:RPE | ||||||
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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Description | Sheep anti Rabbit IgG:FITC | ||||||
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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Description | Goat anti Rabbit IgG (Fc):HRP |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml | Log in | |||
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Description | TidyBlot Western Blot Detection Reagent:HRP |
- Synonyms
- Epidermal Growth Factor
- RRID
- AB_2096118
- UniProt
- P07522
- Entrez Gene
- Egf
- GO Terms
- GO:0005509 calcium ion binding
- GO:0016021 integral to membrane
- GO:0005154 epidermal growth factor receptor binding
- GO:0005625 soluble fraction
- GO:0007173 epidermal growth factor receptor signaling pathway
- GO:0008083 growth factor activity
- GO:0050708 regulation of protein secretion
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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