Dynamin (pSer778) antibody
Sheep anti Rat dynamin (pSer778) antibody (AHP907) used to assess site specific phosphorylation of Dynamin I in rat cerebellar granule neuronal lysates by western blotting
Image caption:
Ts65Dn trisomy has no effect on basal phosphorylation of dynamin I.
Wild-type (Wt) and trisomic (Ts) CGNs were lysed and the phosphorylation status of either Ser774 (PSer774) or Ser778 (PSer778) on dynamin I (DynI) was monitored by western blotting with phospho-specific antibodies. A) Representative blots are displayed from Wt and Ts CGN lysates probed with either PSer774 or PSer778 antibodies and actin loading controls. B) Mean phosphorylation levels of either PSer774 or PSer778 normalised to actin are displayed in either Wt (black bars) or Ts (red bars) CGNs ± SEM. Data are presented normalised to Wt (n = 10 PSer774; n = 11 PSer778, two-tailed unpaired t-test, ns = p > 0.05.
From: Marland JR, Smillie KJ, Cousin MA.
Synaptic Vesicle Recycling Is Unaffected in the Ts65Dn Mouse Model of Down Syndrome.
PLoS One. 2016 Jan 25;11(1):e0147974.
doi: 10.1371/journal.pone.0147974.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
WB ResetSheep anti Dynamin (pSer778)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Dynamin (pSer778)
Antibody Quality and Performance Guarantee
| Sheep anti Rat dynamin (pSer778) antibody recognizes dynamin, also known as B-dynamin and D100, when phosphorylated at serine 778. Dynamin is a phosphoprotein expressed at nerve terminals, which plays an important role in the regulation of synaptic vesicle endocytosis. Cyclin dependent protein kinase 5 (cdk5) has been shown to phosphorylate serine 774 and 778 in vivo. Dephosphorylation of these residues is essential for endocytosis of synaptic vesicles. |
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Bovine Expected from Sequence Chicken Expected from Sequence Human Expected from Sequence Dog Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to phosphorylated rat dynamin were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- 10mM HEPES pH7.5
- Preservative Stabilisers
0.09% Sodium Azide 0.01% Bovine Serum Albumin 50% Glycerol - Immunogen
- Synthetic phosphopeptide corresponding to an amino acid sequence within dynamin which includes phosphorylated Ser778.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- SHEEP ANTI DYNAMIN (pSer778) antibody detects a band of approximately 95 kDa in rat hippocampus lysates.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C E F WB | 1.5 ml |
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| List Price | Your Price | ||||||
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| Description | Rabbit anti Sheep IgG (H/L):Biotin | ||||||
References for Dynamin (pSer778) antibody
-
Marland, J.R. et al. (2016) Synaptic Vesicle Recycling Is Unaffected in the Ts65Dn Mouse Model of Down Syndrome.
PLoS One. 11 (1): e0147974.
- RRID
- AB_566954
- UniProt
- P21575
- Entrez Gene
- Dnm1
- GO Terms
- GO:0005886 plasma membrane
- GO:0003924 GTPase activity
- GO:0003774 motor activity
- GO:0005525 GTP binding
- GO:0005794 Golgi apparatus
- GO:0005874 microtubule
- GO:0008022 protein C-terminus binding
- GO:0042802 identical protein binding
- GO:0031623 receptor internalization
- View More GO Terms
- GO:0032403 protein complex binding
AHP907
147635
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