Darpp-32 (pThr34) antibody
Rabbit anti Darpp-32 (pThr34)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Darpp-32 (pThr34)
Antibody Quality and Performance Guarantee
| Rabbit anti Rat DARPP-32 (pThr34) antibody recognizes DARPP-32, also known as protein phosphatase 1 regulatory subunit 1B and dopamine- and cAMP-regulated neuronal phosphoprotein, when phosphorylated at threonine 34. DARPP-32 is a 205 amino acid ~32 kDa member of the protein phosphatase inhibitor 1 family. DARPP-32 is principally expressed in striatal medium spiny neurons, and plays a critical role in the regulation of dopaminergic neurotransmission. DARPP-32 can act either as a phosphatase inhibitor or as a kinase inhibitor, depending on its relative state of phosphorylation . Phosphorylation at threonine 34 converts DARPP-32 into an inhibitor of protein phosphatase-1 (PP-1) whilst phosphorylation at threonine 75 switches the protein to an inhibitor of protein kinase A (PKA) . G-protein coupled receptor 6 deficiency in a mouse model of Parkinsons disease leads to an increase in DARPP-32 (pThr34) in striatopalladial neurons with a concommitent increase in locomotor activity and reduced abnormal movements in the mouse dyskinesia model of Parkinson's disease, thus suggesting treatment other than dopamine replacement for the condition (Oekl et al. 2014). |
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Dog Expected from Sequence Human Expected from Sequence Bovine Expected from Sequence Chicken Expected from Sequence Monkey Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to phosphorylated rat DARPP-32 were raised by repeated immunisations of rabbits with highly purified antigen.
- Buffer Solution
- 10mM HEPES pH7.5
- Preservative Stabilisers
0.09% Sodium Azide 0.1% Bovine Serum Albumin 50% Glycerol - Immunogen
- Synthetic phosphopeptide corresponding to an amino acid sequence within DARPP-32 which includes phosphorylated Thr34.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/1000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP897 detects a band of approximately 32kDa in rat caudate lysates.
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|---|---|---|---|---|---|---|---|
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| Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
References for Darpp-32 (pThr34) antibody
-
Xiao, M.F. et al. (2009) Neural cell adhesion molecule modulates dopaminergic signaling and behavior by regulating dopamine D2 receptor internalization.
J Neurosci. 29: 14752-63. -
Oeckl, P. et al. (2014) G-protein coupled receptor 6 deficiency alters striatal dopamine and cAMP concentrations and reduces dyskinesia in a mouse model of Parkinson's disease
Exp Neurol. 257C: 1-9. -
Kotarska, A. et al. (2020) Cell adhesion molecule close homolog of L1 binds to the dopamine receptor D2 and inhibits the internalization of its short isoform.
FASEB J. 34 (4): 4832-51.
- Synonyms
- DOPAMINE-AND cAMP-REGULATED PHOSPHOPROTEIN-32
- RRID
- AB_566944
- UniProt
- Q6J4I0
- Entrez Gene
- Ppp1r1b
- GO Terms
- GO:0005102 receptor binding
- GO:0004864 protein phosphatase inhibitor activity
- GO:0035556 intracellular signal transduction
- GO:0005737 cytoplasm
AHP897
153985
170508
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