Connexin 43 antibody

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Rabbit anti Connexin 43 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1157 WB 0.1 ml
Rabbit anti Human Connexin 43 antibody recognizes rat connexin43, a ~43 kDa member of the alpha-type (group II) subfamily of the connexins. It is a component of gap junctions, the small channels linking the cytoplasm of adjacent cells and enabling signalling through the transfer of ions and metabolites. Phosphorylation of Connexin 43 at serine 368 regulates the permeability of the gap junction. Connexin 43 is the major protein of gap junctions in the heart, essential to synchronised contractions. It is also crucial in embryonic development and is associated with epilepsy.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep Expected from Sequence
Zebrafish Expected from Sequence
Human Expected from Sequence
Chicken Expected from Sequence
Mouse Expected from Sequence
Primate Expected from Sequence
Dog Expected from Sequence
Birds Expected from Sequence
Xenopus Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to rat connexin 43 (CT) were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
10mM Hepes pH7.5
150mM NaCl
Preservative Stabilisers
0.09%Sodium Azide
0.01%Bovine Serum Albumin
50%Glycerol
Immunogen
Synthetic peptide corresponding to the C-terminal region of rat connexin 43 (CT).

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

UniProt
P08050 Related reagents
Entrez Gene
Gja1 Related reagents
GO Terms
GO:0006915 apoptosis
GO:0005764 lysosome
GO:0005887 integral to plasma membrane
GO:0005243 gap junction channel activity
GO:0005741 mitochondrial outer membrane
GO:0005769 early endosome
GO:0005771 multivesicular body
GO:0005789 endoplasmic reticulum membrane
GO:0005916 fascia adherens
GO:0005922 connexon complex
GO:0007267 cell-cell signaling
GO:0008285 negative regulation of cell proliferation
GO:0009268 response to pH
GO:0010232 vascular transport
GO:0015867 ATP transport
GO:0016264 gap junction assembly
GO:0017124 SH3 domain binding
GO:0030165 PDZ domain binding
GO:0030660 Golgi-associated vesicle membrane
GO:0043434 response to peptide hormone stimulus
GO:0045121 membrane raft
GO:0045732 positive regulation of protein catabolic process
GO:0048812 neuron projection morphogenesis
GO:0051259 protein oligomerization
GO:0051924 regulation of calcium ion transport
Regulatory
For research purposes only

Applications of Connexin 43 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1157 detects a band of approximately 43kDa in rat cerebellar lysate.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

References for Connexin 43 antibody

  1. Bao, X. et al. (2004) Regulation of purified and reconstituted connexin 43 hemichannels by protein kinase C-mediated phosphorylation of Serine 368.
    J Biol Chem. 279 (19): 20058-66.
  2. Aronica, E. et al. (2001) Expression of connexin 43 and connexin 32 gap-junction proteins in epilepsy-associated brain tumors and in the perilesional epileptic cortex.
    Acta Neuropathol. 101 (5): 449-59.