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Calcitonin Receptor antibody

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Anti Rat Calcitonin Receptor Antibody thumbnail image 1 Anti Rat Calcitonin Receptor Antibody thumbnail image 2
Anti Rat Calcitonin Receptor Antibody gallery image 1
Published customer image:
Rabbit anti Rat Calcitonin Receptor antibody (AHP635) used for the detection of calcitonin receptor (CTR) on murine myofibres by immunofluorescence.
Image caption:
Caveolin-1, integrin α7 and CTR are expressed by Pax7+ve quiescent satellite cells in their niche on the myofibre. Satellite cells in their niche on the surface of freshly isolated EDL myofibres were co-immunostained for Pax7 (green) and either caveolin-1 (a - red), integrin α7 (b - red) or CTR (c - red). All three proteins were expressed in the plasma membrane of Pax7+ve satellite cells (arrowed), clearly revealing their bi-polar morphology. Myofibres were also examined from the masseter of the jaw, and again Pax7+ve satellite cells expressed all three markers (caveolin-1 shown as an example in d). All nuclei were counterstained with DAPI (blue), revealing the presence of the myonuclei of the myofibres on which the satellite cells were located. Scale bar represents 20 μm.

From: Gnocchi VF, White RB, Ono Y, Ellis JA, Zammit PS (2009)
Further Characterisation of the Molecular Signature of Quiescent and Activated Mouse Muscle Satellite Cells.
PLoS ONE 4(4): e5205.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Rat Calcitonin Receptor Antibody gallery image 2
Published customer image:
Rabbit anti Rat Calcitonin Receptor antibody (AHP635) used for the detection of calcitonin receptor (CTR) on murine myofibres by immunofluorescence.
Image caption:
Jagged-1 is induced when satellite cells are activated. EDL myofibres were cultured in suspension for 48 hrs in plating medium to elicit the activation and proliferation of satellite cells (arrows). Activated and proliferating satellite cells were identified by immunostaining for MyoD (green), and Caveolin-1 (a - red), integrin α7 (b - red) and CTR (c - red) remained expressed in the plasma membrane of activated satellite cells. The continued presence of both emerin (red, d) and lamin A/C (green, e) in the nuclear envelope of activated satellite cells expressing MyoD (green in d, red in e) was also noted. Moreover, we found that the Notch ligand Jagged-1 (red) was induced upon activation, in MyoD+ve (green) satellite cells (f). Nuclei were counterstained with DAPI (blue). Scale bar represents 20 μm.

From: Gnocchi VF, White RB, Ono Y, Ellis JA, Zammit PS (2009)
Further Characterisation of the Molecular Signature of Quiescent and Activated Mouse Muscle Satellite Cells.
PLoS ONE 4(4): e5205.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

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Rabbit anti Rat Calcitonin Receptor

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Calcitonin Receptor

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Product Code Applications Pack Size List Price Your Price Qty
AHP635T
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Rabbit anti Rat Calcitonin Receptor antibody recognizes the calcitonin receptor in human, mouse and rat. The calcitonin receptor is a seven transmembrane domain cell surface protein which is coupled to G protein messenger systems. Rabbit anti Rat Calcitonin Receptor antibody recognizes an epitope within the cytoplasmic domain that is common to both C1a and C1b rat isoforms.

Calcitonin receptor is expressed in kidney, central and peripheral nervous systems and in osteoclasts. It is also expressed in a number of tissues during the development of the embryo and may be important in foetal morphogenesis.

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Human
Fish
Atlantic Stingray
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to calcitonin receptor were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Synthetic peptide derived from a C-terminal sequence of rat calcitonin receptor.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Immunohistology - Frozen 1/10 1/50
Immunohistology - Paraffin 1 1/10 1/50
  1. 1The use of 4% paraformaldehyde fixation, rather than formalin fixation is recommended.

    A sensitive detection system is also recommended. e.g. A catalysed signal amplification system.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Kidney, cultured osteoclasts.

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References for Calcitonin Receptor antibody

  1. Tolcos, M. et al. (2003) Ontogeny of calcitonin receptor mRNA and protein in the developing central nervous system of the rat.
    J Comp Neurol. 456 (1): 29-38.
  2. Tikellis, C. et al. (2003) Calcitonin receptor isoforms expressed in the developing rat kidney.
    Kidney Int. 63: 416-26.
  3. Becskei, C. et al. (2004) Immunohistochemical mapping of calcitonin receptors in the adult rat brain.
    Brain Res. 1030: 221-33.
  4. Gelsleichter, J.J. (2006) MCAA: Development of laboratory skills in molecular biology to examine patterns of calcitonin and calcitonin receptor gene expression in cartilaginous fishes.
    Mote technical report 1392.
  5. Wookey, P.J. et al. (2008) Calcitonin receptor immunoreactivity associated with specific cell types in diseased radial and internal mammary arteries.
    Histopathology. 52 (5): 605-12.
  6. Gnocchi, V.F. et al. (2009) Further characterisation of the molecular signature of quiescent and activated mouse muscle satellite cells.
    PLoS One. 4(4):e5205.
  7. Wookey, P.J. (2009) A Review of Calcitonin Receptor Expression in Embryonic, Foetal and Adult Tissues, with an Hypothesis on the Connection Between Expression During Foetal Development and Disease
    The Open Zoology Journal 2: 53-61.
  8. Gooi, J.H. et al. (2010) Calcitonin impairs the anabolic effect of PTH in young rats and stimulates expression of sclerostin by osteocytes.
    Bone. 46: 1486-97.
    9. View The Latest Product References
  9. Wookey, P.J. et al. (2012) Transient expression of the calcitonin receptor by enteric neurons of the embryonic and early post-natal mouse.
    Cell Tissue Res. 347: 311-7.
  10. Mourikis, P. et al. (2012) Cell-autonomous Notch activity maintains the temporal specification potential of skeletal muscle stem cells.
    Development. 139: 4536-48.
  11. Park, J.K. et al. (2012) Increased generation of TRAP expressing multinucleated giant cells in patients with granulomatosis with polyangiitis.
    PLoS One. 7: e42659.
  12. Le Roux, I. et al. (2015) Numb is required to prevent p53-dependent senescence following skeletal muscle injury.
    Nat Commun. 6: 8528.
  13. Yamaguchi, M. et al. (2015) Calcitonin Receptor Signaling Inhibits Muscle Stem Cells from Escaping the Quiescent State and the Niche.
    Cell Rep. 13 (2): 302-14.
  14. Almeida, C.F. et al. (2016) Muscle Satellite Cells: Exploring the Basic Biology to Rule Them.
    Stem Cells Int. 2016: 1078686.

Immunohistology - Paraffin

Western Blotting

RRID
AB_2068968
UniProt
P32214
Entrez Gene
Calcr
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0004948 calcitonin receptor activity
GO:0030424 axon
GO:0032841 calcitonin binding
GO:0043025 neuronal cell body

AHP635T

155226 157827

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