5-alpha Reductase Type 2 antibody

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Rabbit anti Rat 5-alpha Reductase Type 2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
7927-3059
Datasheet
P*
MSDS
50 µl loader

Rabbit anti Rat 5-alpha reductase type 2 antibody recognizes 5-alpha reductase type 2 and does not react with type 1 5-alpha reductase.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Ig fraction - lyophilised
Reconstitution
Reconstitute in 50 µl of sterile distilled water. For long term storage the addition of a preservative is recommended.
Preparation
Ig fraction prepared by ammonium sulphate precipitation
Buffer Solution
Ammonium bicarbonate
Preservative Stabilisers
None present
Immunogen
Synthetic peptide corresponding to a C-terminal portion of native rat 5-alpha reductase type 2 conjugated to human thyroglobulin. The amino acid sequence is QAFYHHRFYLKMFKDYPKSRKAL.

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of reconstitution.

More Information

UniProt
Q28892 Related reagents
GO Terms
GO:0003865 3-oxo-5-alpha-steroid 4-dehydrogenase activity
GO:0016021 integral to membrane
GO:0005789 endoplasmic reticulum membrane
GO:0005792 microsome
GO:0055114 oxidation-reduction process
GO:0007548 sex differentiation
GO:0008202 steroid metabolic process
GO:0030154 cell differentiation
Regulatory
For research purposes only

Applications of 5-alpha Reductase Type 2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Rat prostate

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B C E P WB 1 ml loader
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg loader
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg loader
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml