Tryptophan Hydroxylase antibody
Sheep anti Rabbit tryptophan hydroxylase antibody (9260-2505) used to identify neurons in rat brain by immunohistochemistry on cryostat sections
Image caption:
Low magnification photomicrographs illustrating TrpOH/c-Fos-immunostained sections from different rostrocaudal levels of the DR from a control (CO) rat. TrpOH-immunopositive neurons and dendrites can be identified by the brown/orange precipitate within subdivisions of the DR. The subdivisions of the DR analyzed are illustrated by dashed lines (adapted from a standard stereotaxic atlas of the rat brain [42]). Abbreviations: Aq, cerebral aqueduct; DRC, dorsal raphe nucleus, caudal part; DRD, dorsal raphe nucleus, dorsal part; DRI, dorsal raphe nucleus, interfascicular part; DRV, dorsal raphe nucleus, ventral part; DRVL, dorsal raphe nucleus, ventrolateral part; mlf, medial longitudinal fasciculus; xscp, decussation of the superior cerebellar peduncle. Scale bar, 500μm.
From: Bouwknecht JA, Spiga F, Staub DR, Hale MW, Shekhar A, Lowry CA.
Differential effects of exposure to low-light or high-light open-field on anxiety-related behaviors: relationship to c-Fos expression in serotonergic and non-serotonergic neurons in the dorsal raphe nucleus.
Brain Res Bull. 2007 Apr 2;72(1):32-43.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Sheep anti Rabbit tryptophan hydroxylase antibody (9260-2505) used to identify neurons in rat brain by immunohistochemistry on cryostat sections
Image caption:
Photomicrographs illustrating c-Fos-immunopositive nuclei and TrpOH-immunopositive neurons in the mid-rostrocaudal DRVL (−8.18 mm Bregma) of rats exposed to (A) CO, (B) HA, (C) LL, and (D) HL conditions. Black boxes indicate regions shown at higher magnification in insets in the lower right hand corner of each panel. Arrowheads indicate examples of c-Fos-immunopositive cells (blue/black nuclear staining); arrows indicate TrpOH-immunopositive (serotonergic) neurons (brown/orange cytoplasmic staining). c-Fos-immunopositive/TrpOH-immunopositive neurons were rarely observed. bv, blood vessels characteristic of the DRVL region at this rostrocaudal level. Scale bar, 50 μm; inset: 25 μm.
From: Bouwknecht JA, Spiga F, Staub DR, Hale MW, Shekhar A, Lowry CA.
Differential effects of exposure to low-light or high-light open-field on anxiety-related behaviors: relationship to c-Fos expression in serotonergic and non-serotonergic neurons in the dorsal raphe nucleus.
Brain Res Bull. 2007 Apr 2;72(1):32-43.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Sheep anti Rabbit tryptophan hydroxylase antibody (9260-2505) used for the demonstration of immunoreactive neurons in rat brain by immunohistochemistry on cryostat sections.
Image caption:
Low magnification photomicrographs illustrating TrpOH/c-Fos-immunostained sections from different rostrocaudal levels of the midbrain raphe nuclei from a control rat. TrpOH-ir neurons and dendrites can be identified by the brown/orange precipitate within subdivisions of the DR and MnR. The subdivisions of the DR and MnR analyzed are illustrated by dashed lines (adapted from a standard stereotaxic atlas of the rat brain; Paxinos and Watson, 1998). Abbreviations: Aq, cerebral aqueduct; DRC, dorsal raphe nucleus, caudal part; DRD, dorsal raphe nucleus, dorsal part; DRI, dorsal raphe nucleus, interfascicular part; DRV, dorsal raphe nucleus, ventral part; DRVL, dorsal raphe nucleus, ventrolateral part; mlf, medial longitudinal fasciculus; MnR, median raphe nucleus; xscp, decussation of the superior cerebellar peduncle. Scale bar, 500 μm.
From: Hale MW, Hay-Schmidt A, Mikkelsen JD, Poulsen B, Bouwknecht JA, Evans AK, Stamper CE, Shekhar A, Lowry CA.
Exposure to an open-field arena increases c-Fos expression in a subpopulation of neurons in the dorsal raphe nucleus, including neurons projecting to the basolateral amygdaloid complex.
Neuroscience. 2008 Dec 10;157(4):733-48.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Sheep anti Rabbit tryptophan hydroxylase antibody (9260-2505) used for the demonstration of immunoreactive neurons in rat brain by immunohistochemistry on cryostat sections.
Image caption:
Photomicrographs illustrating the effects of exposure to the open-field in low-light, or high-light conditions compared to home cage controls, on c-Fos expression in serotonergic and non-serotonergic neurons in the mid-rostrocaudal dorsal raphe nucleus (−8.18 mm Bregma). Photomicrographs illustrate c-Fos-ir nuclei and TrpOH-ir neurons in sections from rats exposed to A) CO, B) LL, C) HL conditions in Experiment 1. Black boxes indicate regions shown at higher magnification in insets in the lower left hand corner of each panel. Arrows indicate examples of c-Fos-ir cells (blue/black nuclear staining); white arrowheads indicate c-Fos-immunonegative/TrpOH-ir (serotonergic) neurons (brown/orange cytoplasmic staining), filled arrowheads indicate c-Fos-ir/TrpOH-ir (double-immunostained) neurons. Abbreviation: Aq, cerebral aqueduct; bv, blood vessel, characteristic of the DRVL region at this rostrocaudal level; mlf, medial longitudinal fasciculus. Scale bar, 100 μm, inset 25 μm.
From: Hale MW, Hay-Schmidt A, Mikkelsen JD, Poulsen B, Bouwknecht JA, Evans AK, Stamper CE, Shekhar A, Lowry CA.
Exposure to an open-field arena increases c-Fos expression in a subpopulation of neurons in the dorsal raphe nucleus, including neurons projecting to the basolateral amygdaloid complex.
Neuroscience. 2008 Dec 10;157(4):733-48.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Sheep anti Rabbit tryptophan hydroxylase antibody (9260-2505) used for the demonstration of immunoreactive neurons in rat brain by immunofluorescence on cryostat sections.
Image caption:
Double immunofluorescence photomicrograph illustrating a retrogradely labeled serotonergic neuron in the DRD region of the mid-rostrocaudal dorsal raphe nucleus (-8.18 mm Bregma) following unilateral intra-BL injection of CTb. A) Photomicrograph showing the mid-rostrocaudal dorsal raphe nucleus. White box in A indicates region shown at higher magnification in B. White box in B indicates region shown at higher magnification in C, D and E. TrpOH-ir (serotonergic) neurons appear red (C) while CTb-ir (retrogradely labeled) neuron appears green (D). Double TrpOH/CTb immunofluorescent neuron appears yellow (E). Scale bar (A) 200μm, (B) 80μm, (C), (D) & (E) 20μm.
From: Hale MW, Hay-Schmidt A, Mikkelsen JD, Poulsen B, Bouwknecht JA, Evans AK, Stamper CE, Shekhar A, Lowry CA.
Exposure to an open-field arena increases c-Fos expression in a subpopulation of neurons in the dorsal raphe nucleus, including neurons projecting to the basolateral amygdaloid complex.
Neuroscience. 2008 Dec 10;157(4):733-48.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Sheep anti Rabbit tryptophan hydroxylase antibody (9260-2505) used to identify immunoreactive neurons by immunohistochemistry on cryostat sections.
Image caption:
Influence of M. vaccae or its derivatives on pulmonary cytokine mRNA expression, c-Fos expression in the nTS and c-Fos expression in serotonergic neurons in the dorsal raphe nucleus (DR). (a) Graphs illustrate mean levels, relative to β-actin, of pulmonary IL-1β (IL-1), TNF-α (TNF), and IL-6 mRNA expression 12 h, and 3, 6, 10, and 17 days following i.t. injection of Mv-NC in M. vaccae–preimmunized mice (•) relative to cytokine mRNA expression following i.t. injection of vehicle in M. vaccae–preimmunized mice (O) (12 h time point only), as well as, for comparison, cytokine mRNA expression at the same time points following i.t. injection of OVA-NC in OVA/alum-preimmunized mice (▼) or vehicle in OVA/alum-preimmunized mice (▽) (12 h time point only). (b) Bar graphs illustrate the mean number (±S.E.M.) of c-Fos-ir nuclei in the AP, SolDL, DRC, and DRI in experiment 1. (c) Photographs illustrate nuclear c-Fos immunostaining (blue–black) 12 h following i.t. injection of M. vaccae in M. vaccae–preimmunized mice in the AP and SolDL of the nTS (top two photographs) and serotonergic neurons in the DRC (middle two photographs) and DRI parts (bottom two photographs) of the DR. Tyrosine hydroxylase immunostaining (brown) was used to aid in identification of neuroanatomical subdivisions of the nTS. Tryptophan hydroxylase immunostaining (brown) was used to identify serotonergic neurons in the DRC and DRI. (⇒) c-Fos-immunonegative serotonergic neurons, (→) c-Fos-immunopositive serotonergic neurons. Small black boxes in (c) indicate regions shown at higher magnification in insets. Scale bar=100 μm (c) top row; (c) middle and bottom rows, 25 μm; (c) insets, 12.5 μm. Abbreviations: IL-1, interleukin-1β; (Mv), preimmunization with s.c. injections of heat-killed M. vaccae; Mv-NC, i.t. challenge with sonicated heat-killed Mv-NC; NC, i.t. challenge with NC. ' P≤0.05, compared with M. vaccae–preimmunized, vehicle-injected controls, Fisher's protected LSD test. For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.
From: Lowry CA, Hollis JH, de Vries A, Pan B, Brunet LR, Hunt JR, Paton JF, van Kampen E, Knight DM, Evans AK, Rook GA, Lightman SL.
Identification of an immune-responsive mesolimbocortical serotonergic system: potential role in regulation of emotional behavior.
Neuroscience. 2007 May 11;146(2):756-72.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Sheep anti Rabbit tryptophan hydroxylase antibody (9260-2505) used to identify immunoreactive neurons by immunohistochemistry on cryostat sections.
Image caption:
Both i.t. Mv-NC and i.t. OVA-NC induced c-Fos expression in the nTS but only Mv-NC increased c-Fos expression in DRI serotonergic neurons. Immunostained products are the same as in Fig. 1. (a) Bar graphs illustrate the mean number (±S.E.M.) of c-Fos-ir nuclei in the AP and SolDL (left) and c-Fos-ir/tryptophan hydroxylase-ir neurons in the DRC and DRI (right) in experiment 2. (b, c) Photomicrographs illustrate c-Fos responses to Mv-NC in M. vaccae–preimmunized mice or to OVA-NC in OVA/alum-preimmunized mice in the SolDL and AP (b) or the DRI (c). (⇒) c-Fos-immunonegative serotonergic neurons, (→) c-Fos-immunopositive serotonergic neurons. Small black boxes indicate regions shown at higher magnification in insets. Scale bar=100 μm b; b (insets), 50 μm; c, 25 μm; c (insets) 12.5 μm. Abbreviations: (OVA), preimmunization with s.c. injections of OVA/alum; OVA-NC, i.t. challenge with OVA-NC. For additional abbreviations, see Fig. 1 legend. * P≤0.05, compared with the appropriate M. vaccae– or OVA/alum-preimmunized, vehicle-injected control group, Fisher's protected LSD test.
From: Lowry CA, Hollis JH, de Vries A, Pan B, Brunet LR, Hunt JR, Paton JF, van Kampen E, Knight DM, Evans AK, Rook GA, Lightman SL.
Identification of an immune-responsive mesolimbocortical serotonergic system: potential role in regulation of emotional behavior.
Neuroscience. 2007 May 11;146(2):756-72.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Sheep anti Rabbit tryptophan hydroxylase antibody (9260-2505) used to identify immunoreactive neurons by immunohistochemistry on cryostat sections.
Image caption:
Activation of bronchopulmonary afferent vagal pathways was not necessary for the effects of Mv-NC on DRI serotonergic neurons. Immunostained products are the same as in Fig. 1. (a) Bar graphs illustrate the mean number (±S.E.M.) of c-Fos-ir nuclei in the AP and SolDL (left) and c-Fos-ir/tryptophan hydroxylase-ir neurons in the DRC and DRI (right) in experiment 4. (b, c) Photomicrographs illustrate c-Fos responses to i.t. Mv-NC or s.c. M. vaccae in M. vaccae–preimmunized mice in the SolDL and AP (b) or the DRI (c) at the 12 h time point. For abbreviations, see Fig. 1 legend. (⇒) c-Fos-immunonegative serotonergic neurons, (→) c-Fos-immunopositive serotonergic neurons. Scale bar=100 μm b; b (insets), 50 μm; c, 25 μm; c (insets), 12.5 μm. Abbreviations: Mv, s.c. challenge with heat-killed M. vaccae; Sal, s.c. challenge with saline vehicle. For additional abbreviations, see Fig. 1 legend. * P≤0.05, compared with the appropriate M. vaccae–preimmunized, vehicle-injected control group, Fisher's protected LSD test.
From: Lowry CA, Hollis JH, de Vries A, Pan B, Brunet LR, Hunt JR, Paton JF, van Kampen E, Knight DM, Evans AK, Rook GA, Lightman SL.
Identification of an immune-responsive mesolimbocortical serotonergic system: potential role in regulation of emotional behavior.
Neuroscience. 2007 May 11;146(2):756-72.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Sheep anti Rabbit tryptophan hydroxylase antibody (9260-2505) used for immunostaining neurons in rat brain by immunohistochemistry on cryostat sections.
Image caption:
Fos expression in TrpOH-immunostained neurons in the DRN/MnR and ChAT-immunostained neurons in the PPT/LDT. A. Illustrations of brain sections of the DRN and MnR. B. Photomicrograph illustrating an example of Fos expression in TrpOH-immunostained neurons in the DRN and MnR. Scale bars = 100 μm. C and D. Fos expression positive ratio in serotonergic neurons in the DRN, there were no difference among groups. E and F. Fos expression positive ratio in serotonergic neurons in the MnR, there were no difference among groups. G and I. Illustrations of brain sections of the PPT and LDT. H and J. Photomicrograph illustrating an example of Fos expression in ChAT-immunostained neurons in the PPT and LDT. Scale bars = 100 μm. K and L. Fos expression positive ratio in cholinergic neurons in the PPT, there were no difference among groups. M and N. Fos expression positive ratio in cholinergic neurons in the LDT, there were no difference among groups. Data are represented as mean ± SEM.
From: Yu B, Cui SY, Zhang XQ, Cui XY, Li SJ, Sheng ZF, Cao Q, Huang YL, Xu YP, Lin ZG, Yang G, Song JZ, Ding H, Zhang YH.
Mechanisms Underlying Footshock and Psychological Stress-Induced Abrupt Awakening From Posttraumatic "Nightmares".
Int J Neuropsychopharmacol. 2015 Nov 20. pii: pyv113.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Sheep anti Rabbit tryptophan hydroxylase antibody recognizes tryptophan hydroxylase, also known as Tryptophan 5-hydroxylase 1 or Tryptophan 5-monooxygenase 1. Tryptophan hydroxylase is a 444 amino acid ~55 kDa enzyme involved in the synthesis of the neurotransmitter serotonin.
Product Details
- Target Species
- Rabbit
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Human Mammals Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified Ig - liquid
- Preparation
- Purified Ig prepared by affinity chromatography
- Buffer Solution
- HEPES buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
50% Glycerol
0.01% Bovine Serum Albumin. - Immunogen
- Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P17290
- GO Terms
- GO:0004510 tryptophan 5-monooxygenase activity
- GO:0005506 iron ion binding
- GO:0055114 oxidation-reduction process
- GO:0009072 aromatic amino acid family metabolic process
- GO:0016597 amino acid binding
- GO:0042427 serotonin biosynthetic process
- Regulatory
- For research purposes only
Applications of Tryptophan Hydroxylase antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- Immunohistology
- 9260-2505 has been used for immunohistochemistry on formaldehyde fixed tissue, it is useful for cell body but not fiber staining
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
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|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C E F WB | 1.5 ml |
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| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F IF | 0.1 mg |
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| Donkey anti Sheep/Goat IgG:HRP | STAR88P | C E P WB | 1 ml |
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Product Specific References
References for Tryptophan Hydroxylase antibody
-
Lowry, C.A. et al. (2000) Corticotropin-releasing factor increases in vitro firing rates of serotonergic neurons in the rat dorsal raphe nucleus: evidence for activation of a topographically organized mesolimbocortical serotonergic system.
J Neurosci. 20 (20): 7728-36. -
Federici, L.M. et al. (2016) Hypothalamic orexin's role in exacerbated cutaneous vasodilation responses to an anxiogenic stimulus in a surgical menopause model.
Psychoneuroendocrinology. 65: 127-37. -
Johnson, P.L. et al. (2005) Acute hypercarbic gas exposure reveals functionally distinct subpopulations of serotonergic neurons in rats.
J Psychopharmacol. 19 (4): 327-41. -
Sajdyk, T.J. et al. (2008) Neuropeptide Y in the amygdala induces long-term resilience to stress-induced reductions in social responses but not hypothalamic-adrenal-pituitary axis activity or hyperthermia.
J Neurosci. 28 (4): 893-903. -
Johnson, P. et al. (2008) Disruption of GABAergic tone in the dorsomedial hypothalamus attenuates responses in a subset of serotonergic neurons in the dorsal raphe nucleus following lactate-induced panic.
J Psychopharmacol. 22 (6): 642-52. -
Yu B et al. (2015) Mechanisms Underlying Footshock and Psychological Stress-Induced Abrupt Awakening From Posttraumatic "Nightmares".
Int J Neuropsychopharmacol. Nov 20. pii: pyv113. [Epub ahead of print] -
Abrams, J.K. et al. (2005) Serotonergic systems associated with arousal and vigilance behaviors following administration of anxiogenic drugs.
Neuroscience. 133 (4): 983-97. -
Staub, D.R. et al. (2005) Urocortin 2 increases c-Fos expression in topographically organized subpopulations of serotonergic neurons in the rat dorsal raphe nucleus.
Brain Res. 1044 (2): 176-89. -
Cui, S.Y. et al. (2011) Diltiazem potentiates pentobarbital-induced hypnosis via 5-HT1A and 5-HT2A/2C receptors: role for dorsal raphe nucleus.
Pharmacol Biochem Behav. 99 (4): 566-72. -
Bouwknecht, J.A. et al. (2007) Differential effects of exposure to low-light or high-light open-field on anxiety-related behaviors: relationship to c-Fos expression in serotonergic and non-serotonergic neurons in the dorsal raphe nucleus.
Brain Res Bull. 72 (1): 32-43. -
Gardner, K.L. et al. (2005) Early life experience alters behavior during social defeat: focus on serotonergic systems.
Neuroscience. 136 (1): 181-91. -
Hale, M.W. et al. (2008) Exposure to an open-field arena increases c-Fos expression in a subpopulation of neurons in the dorsal raphe nucleus, including neurons projecting to the basolateral amygdaloid complex.
Neuroscience. 157 (4): 733-48. -
Spiga, F. et al. (2006) Injections of urocortin 1 into the basolateral amygdala induce anxiety-like behavior and c-Fos expression in brainstem serotonergic neurons.
Neuroscience. 138 (4): 1265-76. -
Rook, G. et al. (2003) Treatment of conditions of the central nervous system using mycobacteria
U.S. Patent Publication US20030170275 A1 -
Staub, D.R. et al. (2006) Evidence supporting a role for corticotropin-releasing factor type 2 (CRF2) receptors in the regulation of subpopulations of serotonergic neurons.
Brain Res. 1070 (1): 77-89. -
Lowry, C.A. et al. (2007) Identification of an immune-responsive mesolimbocortical serotonergic system: potential role in regulation of emotional behavior.
Neuroscience. 146 (2): 756-72.
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