Sheep anti Rabbit tryptophan hydroxylase antibody recognizes tryptophan hydroxylase, also known as Tryptophan 5-hydroxylase 1 or Tryptophan 5-monooxygenase 1. Tryptophan hydroxylase is a 444 amino acid ~55 kDa enzyme involved in the synthesis of the neurotransmitter serotonin.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified Ig - liquid
- Purified Ig prepared by affinity chromatography
- Buffer Solution
- HEPES buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
0.01% Bovine Serum Albumin.
- Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- GO Terms
tryptophan 5-monooxygenase activity
iron ion binding
aromatic amino acid family metabolic process
amino acid binding
serotonin biosynthetic process
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Tryptophan Hydroxylase antibody
|Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- 9260-2505 has been used for immunohistochemistry on formaldehyde fixed tissue, it is useful for cell body but not fiber staining
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Application Based External Images
Immunohistology - Frozen
Product Specific References
References for Tryptophan Hydroxylase antibody
Lowry, C.A. et al. (2000) Corticotropin-releasing factor increases in vitro firing rates of serotonergic neurons in the rat dorsal raphe nucleus: evidence for activation of a topographically organized mesolimbocortical serotonergic system.
J Neurosci. 20 (20): 7728-36.
Federici, L.M. et al. (2016) Hypothalamic orexin's role in exacerbated cutaneous vasodilation responses to an anxiogenic stimulus in a surgical menopause model.
Psychoneuroendocrinology. 65: 127-37.
Johnson, P.L. et al. (2005) Acute hypercarbic gas exposure reveals functionally distinct subpopulations of serotonergic neurons in rats.
J Psychopharmacol. 19 (4): 327-41.
Sajdyk, T.J. et al. (2008) Neuropeptide Y in the amygdala induces long-term resilience to stress-induced reductions in social responses but not hypothalamic-adrenal-pituitary axis activity or hyperthermia.
J Neurosci. 28 (4): 893-903.
Johnson, P. et al. (2008) Disruption of GABAergic tone in the dorsomedial hypothalamus attenuates responses in a subset of serotonergic neurons in the dorsal raphe nucleus following lactate-induced panic.
J Psychopharmacol. 22 (6): 642-52.
Yu B et al. (2015) Mechanisms Underlying Footshock and Psychological Stress-Induced Abrupt Awakening From Posttraumatic "Nightmares".
Int J Neuropsychopharmacol. Nov 20. pii: pyv113. [Epub ahead of print]
Abrams, J.K. et al. (2005) Serotonergic systems associated with arousal and vigilance behaviors following administration of anxiogenic drugs.
Neuroscience. 133 (4): 983-97.
Staub, D.R. et al. (2005) Urocortin 2 increases c-Fos expression in topographically organized subpopulations of serotonergic neurons in the rat dorsal raphe nucleus.
Brain Res. 1044 (2): 176-89.
Cui, S.Y. et al. (2011) Diltiazem potentiates pentobarbital-induced hypnosis via 5-HT1A and 5-HT2A/2C receptors: role for dorsal raphe nucleus.
Pharmacol Biochem Behav. 99 (4): 566-72.
Bouwknecht, J.A. et al. (2007) Differential effects of exposure to low-light or high-light open-field on anxiety-related behaviors: relationship to c-Fos expression in serotonergic and non-serotonergic neurons in the dorsal raphe nucleus.
Brain Res Bull. 72 (1): 32-43.
Gardner, K.L. et al. (2005) Early life experience alters behavior during social defeat: focus on serotonergic systems.
Neuroscience. 136 (1): 181-91.
Hale, M.W. et al. (2008) Exposure to an open-field arena increases c-Fos expression in a subpopulation of neurons in the dorsal raphe nucleus, including neurons projecting to the basolateral amygdaloid complex.
Neuroscience. 157 (4): 733-48.
Spiga, F. et al. (2006) Injections of urocortin 1 into the basolateral amygdala induce anxiety-like behavior and c-Fos expression in brainstem serotonergic neurons.
Neuroscience. 138 (4): 1265-76.
Rook, G. et al. (2003) Treatment of conditions of the central nervous system using mycobacteria
U.S. Patent Publication US20030170275 A1
Staub, D.R. et al. (2006) Evidence supporting a role for corticotropin-releasing factor type 2 (CRF2) receptors in the regulation of subpopulations of serotonergic neurons.
Brain Res. 1070 (1): 77-89.
Lowry, C.A. et al. (2007) Identification of an immune-responsive mesolimbocortical serotonergic system: potential role in regulation of emotional behavior.
Neuroscience. 146 (2): 756-72.