Tryptophan Hydroxylase antibody

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Sheep anti Rabbit Tryptophan Hydroxylase

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
9260-2505
Datasheet
C WB
MSDS
25 µl

Sheep anti Rabbit tryptophan hydroxylase antibody recognizes tryptophan hydroxylase, also known as Tryptophan 5-hydroxylase 1 or Tryptophan 5-monooxygenase 1. Tryptophan hydroxylase is a 444 amino acid ~55 kDa enzyme involved in the synthesis of the neurotransmitter serotonin.

Product Details

Target Species
Rabbit
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Human
Mammals Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified Ig - liquid
Preparation
Purified Ig prepared by affinity chromatography
Buffer Solution
HEPES buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
50% Glycerol
0.01% Bovine Serum Albumin.
Immunogen
Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P17290 Related reagents
GO Terms
GO:0004510 tryptophan 5-monooxygenase activity
GO:0005506 iron ion binding
GO:0055114 oxidation-reduction process
GO:0009072 aromatic amino acid family metabolic process
GO:0016597 amino acid binding
GO:0042427 serotonin biosynthetic process
Regulatory
For research purposes only

Applications of Tryptophan Hydroxylase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Immunohistology
9260-2505 has been used for immunohistochemistry on formaldehyde fixed tissue, it is useful for cell body but not fiber staining

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 C E F WB 1.5 ml loader
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA F IF 0.1 mg loader
Donkey anti Sheep/Goat IgG:FITC STAR88F C 1 mg loader
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader

Application Based External Images

Immunohistology - Frozen

Product Specific References

References for Tryptophan Hydroxylase antibody

  1. Lowry, C.A. et al. (2000) Corticotropin-releasing factor increases in vitro firing rates of serotonergic neurons in the rat dorsal raphe nucleus: evidence for activation of a topographically organized mesolimbocortical serotonergic system.
    J Neurosci. 20 (20): 7728-36.
  2. Federici, L.M. et al. (2016) Hypothalamic orexin's role in exacerbated cutaneous vasodilation responses to an anxiogenic stimulus in a surgical menopause model.
    Psychoneuroendocrinology. 65: 127-37.
  3. Johnson, P.L. et al. (2005) Acute hypercarbic gas exposure reveals functionally distinct subpopulations of serotonergic neurons in rats.
    J Psychopharmacol. 19 (4): 327-41.
  4. Sajdyk, T.J. et al. (2008) Neuropeptide Y in the amygdala induces long-term resilience to stress-induced reductions in social responses but not hypothalamic-adrenal-pituitary axis activity or hyperthermia.
    J Neurosci. 28 (4): 893-903.
  5. Johnson, P. et al. (2008) Disruption of GABAergic tone in the dorsomedial hypothalamus attenuates responses in a subset of serotonergic neurons in the dorsal raphe nucleus following lactate-induced panic.
    J Psychopharmacol. 22 (6): 642-52.
  6. Yu B et al. (2015) Mechanisms Underlying Footshock and Psychological Stress-Induced Abrupt Awakening From Posttraumatic "Nightmares".
    Int J Neuropsychopharmacol. Nov 20. pii: pyv113. [Epub ahead of print]
  7. Abrams, J.K. et al. (2005) Serotonergic systems associated with arousal and vigilance behaviors following administration of anxiogenic drugs.
    Neuroscience. 133 (4): 983-97.
  8. Staub, D.R. et al. (2005) Urocortin 2 increases c-Fos expression in topographically organized subpopulations of serotonergic neurons in the rat dorsal raphe nucleus.
    Brain Res. 1044 (2): 176-89.
  9. Cui, S.Y. et al. (2011) Diltiazem potentiates pentobarbital-induced hypnosis via 5-HT1A and 5-HT2A/2C receptors: role for dorsal raphe nucleus.
    Pharmacol Biochem Behav. 99 (4): 566-72.
  10. Bouwknecht, J.A. et al. (2007) Differential effects of exposure to low-light or high-light open-field on anxiety-related behaviors: relationship to c-Fos expression in serotonergic and non-serotonergic neurons in the dorsal raphe nucleus.
    Brain Res Bull. 72 (1): 32-43.
  11. Gardner, K.L. et al. (2005) Early life experience alters behavior during social defeat: focus on serotonergic systems.
    Neuroscience. 136 (1): 181-91.
  12. Hale, M.W. et al. (2008) Exposure to an open-field arena increases c-Fos expression in a subpopulation of neurons in the dorsal raphe nucleus, including neurons projecting to the basolateral amygdaloid complex.
    Neuroscience. 157 (4): 733-48.
  13. Spiga, F. et al. (2006) Injections of urocortin 1 into the basolateral amygdala induce anxiety-like behavior and c-Fos expression in brainstem serotonergic neurons.
    Neuroscience. 138 (4): 1265-76.
  14. Rook, G. et al. (2003) Treatment of conditions of the central nervous system using mycobacteria
    U.S. Patent Publication US20030170275 A1
  15. Staub, D.R. et al. (2006) Evidence supporting a role for corticotropin-releasing factor type 2 (CRF2) receptors in the regulation of subpopulations of serotonergic neurons.
    Brain Res. 1070 (1): 77-89.
  16. Lowry, C.A. et al. (2007) Identification of an immune-responsive mesolimbocortical serotonergic system: potential role in regulation of emotional behavior.
    Neuroscience. 146 (2): 756-72.