IgG IgM antibody

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Goat anti Rabbit IgG/IgM:Biotin

Goat anti Rabbit IgG/IgM:HRP

Goat anti Rabbit IgG/IgM:RPE

Goat anti Rabbit IgG/IgM:Texas Red®

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
401008 E 1 mg
401005 E 1 ml
401009 F 0.5 mg
401007 F 1 mg
Goat anti Rabbit IgG/IgM antibody reacts with the heavy and light chains of rabbit IgG and IgM and with the light chains of rabbit IgA as demonstrated by ELISA and flow cytometry.

Product Details

Target Species
Rabbit
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Ig Fraction conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Product Form
Purified Ig conjugated to Texas Red®- liquid
Antiserum Preparation
Antisera to rabbit IgG+IgM were raised by repeated immunisations of goats with normal rabbit IgG and IgM.
The Ig fraction was purified by affinity chromatography on pooled rabbit IgG + IgM covalently linked to agarose.
The preparation has been cross absorbed with pooled human sera and purified human paraproteins to remove any potential cross reactivity.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Preservative Stabilisers
50% glycerol
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Stabilizing agent
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Normal rabbit IgG + IgM.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml
Approx. Protein Concentrations
Ig concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Shelf Life
Please see label for expiry date.
Shelf Life
Please see label for expiry date.
Shelf Life
Please see label for expiry date.
Shelf Life
Please see label for expiry date

More Information

UniProt
P01780 Related reagents
P03988 Related reagents
P04221 Related reagents
GO Terms
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0006958 complement activation, classical pathway
GO:0045087 innate immune response
GO:0005886 plasma membrane
GO:0016021 integral to membrane
Acknowledgements
Texas Red® is a registered trademark of Molecular Probes Inc.
Regulatory
For research purposes only

Applications of IgG IgM antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000 1/10,000
ELISA 1/2000 1/8000
Flow Cytometry 1/5 1/50
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E