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IgG antibody

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Sheep anti Rabbit IgG Antibody thumbnail image 1 Sheep anti Rabbit IgG Antibody thumbnail image 2 Sheep anti Rabbit IgG Antibody thumbnail image 3 Sheep anti Rabbit IgG Antibody thumbnail image 4 Sheep anti Rabbit IgG Antibody thumbnail image 5 Sheep anti Rabbit IgG Antibody thumbnail image 6
Sheep anti Rabbit IgG Antibody gallery image 1
HeLA cells were fixed with 3% PFA and permeabilized with 0.3% Triton X-100. Nuclei were stained with 1.5 µg/ml DAPI. Immunofluorescence was carried out using AHP2167, a Rabbit polyclonal anti-Human Microtubule associated protein 1LC3A/B (MAP1LC3A/B) at 1/25. STAR36D488GA, Sheep anti-Rabbit DyLight®488 was then applied at 1/50. Image was visualized on the Bio-Rad ZOE™ Fluorescent Cell Imager at 60x magnification.
Sheep anti Rabbit IgG Antibody gallery image 2
Multiplex staining of HeLa whole cell lysate with Mouse anti PCNA (MCA1558) detected with Goat anti Mouse IgG (H/L):DyLight®680 (STAR117D680GA) shown in red and Rabbit anti GAPDH (AHP1628) detected with Sheep anti Rabbit IgG:DyLight®488 (STAR36D488GA) shown in green.
Sheep anti Rabbit IgG Antibody gallery image 3
Dylight®649 conjugated Sheep anti Rabbit IgG antibody (STAR36D649) used to detect bound Rabbit anti Human GAPDH antibody (AHP1628). Bound Mouse anti Human PCNA antibody (MCA1558 ) was simultaneously detected with Dylight®549) conjugated Goat anti Mouse IgG antibody (STAR117D549GA )
Sheep anti Rabbit IgG Antibody gallery image 4
Dylight®800 conjugated Sheep anti Rabbit antibody (STAR36D800) used to detect bound Rabbit anti Human GAPDH antibody (AHP1628). Cyclophilin A was detected using Dylight®680 conjugated Human anti Human cyclophilin A antibody
Sheep anti Rabbit IgG Antibody gallery image 5
Multiplex staining of HeLa whole cell lysate with Mouse anti PCNA (MCA1558) detected with Goat anti Mouse IgG (H/L):DyLight®488 (STAR117D488GA) shown in green and Rabbit anti GAPDH (AHP1628) detected with Sheep anti Rabbit IgG:DyLight®680 (STAR36D680GA) shown in red.
Sheep anti Rabbit IgG Antibody gallery image 6
HeLa cells were treated with BrdU for 3 hours and stained with mouse anti-BrdU antibody, clone Bu20a (MCA2483). Goat anti mouse IgG (H/L) DyLight® 549 conjugated antibody (red) (STAR117D549GA) was used as the secondary antibody. Cytoplasm was stained with rabbit anti-GAPDH antibody (AHP1628). As the secondary antibody, sheep anti rabbit IgG DyLight® 488 conjugated antibody (green) (STAR36D488GA) was used. PureBlu™ DAPI (1351303) was used as nuclear counterstain (blue)

Sheep anti Rabbit IgG

Sheep anti Rabbit IgG:DyLight®488

Sheep anti Rabbit IgG:Dylight®800

Sheep anti Rabbit IgG:DyLight®680

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
STAR36B
Datasheet
C E WB
MSDS
1 mg loader

STAR36D488GA
Datasheet
F IF WB
MSDS
0.1 mg

STAR36D800GA
Datasheet
F IF WB
MSDS
0.1 mg

STAR36D680GA
Datasheet
F IF WB
MSDS
0.1 mg

Sheep anti Rabbit IgG antibody recognizes rabbit IgG. In ELISA techniques <5% cross-reactivity is seen with human, mouse, rat or bovine serum.

Product Details

Target Species
Rabbit
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to DyLight®488 - liquid
Product Form
Purified IgG conjugated to DyLight®800 - liquid
Product Form
Purified IgG conjugated to DyLight®680 - liquid
Antiserum Preparation
Antisera to rabbit IgG were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was was prepared from whole serum by affinity chromatography
Buffer Solution
Glycine buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.1%E-amino-n-Caproic Acid
0.01%Benzamidine
1mMEDTA
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Purified Rabbit IgG.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of despatch
Guarantee
18 months from date of despatch
Guarantee
18 months from date of despatch

More Information

UniProt
P01870 Related reagents
Entrez Gene
LOC100009097 Related reagents
GO Terms
GO:0003823 antigen binding
Acknowledgements
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Acknowledgements
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries
Acknowledgements
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen
Western Blotting 1/1000
Flow Cytometry 1/10 1/400
Immunofluorescence
Western Blotting 1/1000 1/10,000
Flow Cytometry
Immunofluorescence
Western Blotting 1/500 1/1000
Flow Cytometry 1/50 1/200
Immunofluorescence 1/50 1/200
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 50ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 50ul of the suggested working dilution to label 1x106 cells in 100ul
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 C E F WB 1.5 ml loader
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA F IF 0.1 mg loader
Donkey anti Sheep/Goat IgG:FITC STAR88F C 1 mg loader
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader

Product Specific References

References for IgG antibody

  1. Heinrich, F. et al. (2015) Immunophenotyping of immune cell populations in the raccoon (Procyon lotor).
    Vet Immunol Immunopathol. 168 (3-4): 140-6.
  2. Tartour, K. et al. (2014) IFITM proteins are incorporated onto HIV-1 virion particles and negatively imprint their infectivity.
    Retrovirology. 11: 103.
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