IgG antibody

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Sheep anti Rabbit IgG:DyLight®488

Sheep anti Rabbit IgG:Dylight®800

Sheep anti Rabbit IgG:DyLight®680

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
STAR36D488GA
Datasheet
F IF WB
SDS
0.1 mg

STAR36D800GA
Datasheet
F IF WB
SDS
0.1 mg

STAR36D680GA
Datasheet
F IF WB
SDS
0.1 mg

Sheep anti Rabbit IgG antibody recognizes rabbit IgG. In ELISA techniques <5% cross-reactivity is seen with human, mouse, rat or bovine serum.

Product Details

Target Species
Rabbit
Product Form
Purified IgG conjugated to DyLight®488 - liquid
Product Form
Purified IgG conjugated to DyLight®800 - liquid
Product Form
Purified IgG conjugated to DyLight®680 - liquid
Antiserum Preparation
Antisera to rabbit IgG were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was was prepared from whole serum by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Purified Rabbit IgG.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P01870
Entrez Gene
LOC100009097
GO Terms
GO:0003823 antigen binding
Acknowledgements
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Acknowledgements
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries
Acknowledgements
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/400
Immunofluorescence
Western Blotting 1/1000 1/10,000
Flow Cytometry
Immunofluorescence
Western Blotting 1/500 1/1000
Flow Cytometry 1/50 1/200
Immunofluorescence 1/50 1/200
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 50ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 50ul of the suggested working dilution to label 1x106 cells in 100ul

Product Specific References

References for IgG antibody

  1. Heinrich, F. et al. (2015) Immunophenotyping of immune cell populations in the raccoon (Procyon lotor).
    Vet Immunol Immunopathol. 168 (3-4): 140-6.
  2. Tartour, K. et al. (2014) IFITM proteins are incorporated onto HIV-1 virion particles and negatively imprint their infectivity.
    Retrovirology. 11: 103.
  3. Lupicka, M. et al. (2019) Effect of ovarian steroids on vascular endothelial growth factor a expression in bovine uterine endothelial cells during adenomyosis.
    BMC Vet Res. 15 (1): 473.

Fluorescent Spectraviewer

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How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra