IgG antibody

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Sheep anti Rabbit IgG Antibody thumbnail image 2

Sheep anti Rabbit IgG Antibody thumbnail image 3

Sheep anti Rabbit IgG Antibody thumbnail image 4

Sheep anti Rabbit IgG Antibody thumbnail image 5

Sheep anti Rabbit IgG Antibody thumbnail image 6

Sheep anti Rabbit IgG Antibody gallery image 1 — HeLA cells were fixed with 3% PFA and permeabilized with 0.3% Triton X-100. Nuclei were stained with 1.5 µg/ml DAPI. Immunofluorescence was carried out using AHP2167, a Rabbit polyclonal anti-Human Microtubule associated protein 1LC3A/B (MAP1LC3A/B) at 1/25. **STAR36D488GA**, Sheep anti-Rabbit DyLight^®488 was then applied at 1/50. Image was visualized on the Bio-Rad ZOE^™ Fluorescent Cell Imager at 60x magnification.

Sheep anti Rabbit IgG Antibody gallery image 2 — Multiplex staining of HeLa whole cell lysate with Mouse anti PCNA (MCA1558) detected with Goat anti Mouse IgG (H/L):DyLight®680 (STAR117D680GA) shown in red and Rabbit anti GAPDH (AHP1628) detected with Sheep anti Rabbit IgG:DyLight®488 (**STAR36D488GA**) shown in green.

Sheep anti Rabbit IgG Antibody gallery image 3 — Dylight^®649 conjugated Sheep anti Rabbit IgG antibody (**STAR36D649**) used to detect bound Rabbit anti Human GAPDH antibody (AHP1628). Bound Mouse anti Human PCNA antibody (MCA1558 ) was simultaneously detected with Dylight^®549) conjugated Goat anti Mouse IgG antibody (STAR117D549GA )

Sheep anti Rabbit IgG Antibody gallery image 4 — Dylight^®800 conjugated Sheep anti Rabbit antibody (**STAR36D800**) used to detect bound Rabbit anti Human GAPDH antibody (AHP1628). Cyclophilin A was detected using Dylight^®680 conjugated Human anti Human cyclophilin A antibody

Sheep anti Rabbit IgG

Sheep anti Rabbit IgG:DyLight®488

Sheep anti Rabbit IgG:Dylight®800

Sheep anti Rabbit IgG:DyLight®680

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size
STAR36B	C E WB	1 mg
STAR36D488GA	F IF WB	0.1 mg
STAR36D800GA	F IF WB	0.1 mg
STAR36D680GA	F IF WB	0.1 mg

Sheep anti Rabbit IgG antibody recognizes rabbit IgG. In ELISA techniques <5% cross-reactivity is seen with human, mouse, rat or bovine serum.

Product Details

Target Species

Rabbit

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to DyLight^®488 - liquid

Product Form

Purified IgG conjugated to DyLight^®800 - liquid

Product Form

Purified IgG conjugated to DyLight^®680 - liquid

Antiserum Preparation

Antisera to rabbit IgG were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was was prepared from whole serum by affinity chromatography

Buffer Solution

Glycine buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
0.1%	E-amino-n-Caproic Acid
0.01%	Benzamidine
1mM	EDTA

Preservative Stabilisers

0.09%

Sodium Azide (NaN₃)

Preservative Stabilisers

0.09%

Sodium Azide (NaN₃)

Preservative Stabilisers

0.09%

Sodium Azide (NaN₃)

Immunogen

Purified Rabbit IgG.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch
Guarantee: 18 months from date of despatch
Guarantee: 18 months from date of despatch

More Information

UniProt: P01870 Related reagents
Entrez Gene: LOC100009097 Related reagents
GO Terms: GO:0003823 antigen binding
Acknowledgements: DyLight^® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Acknowledgements: DyLight^® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries
Acknowledgements: DyLight^® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Regulatory: For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA
Immunohistology - Frozen
Western Blotting		1/1000
Flow Cytometry	1/10	1/400
Immunofluorescence
Western Blotting	1/1000	1/10,000
Flow Cytometry
Immunofluorescence
Western Blotting	1/500	1/1000
Flow Cytometry	1/50	1/200
Immunofluorescence	1/50	1/200
Western Blotting

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 50ul of the suggested working dilution to label 1x10⁶ cells in 100ul
Flow Cytometry: Use 50ul of the suggested working dilution to label 1x10⁶ cells in 100ul
Flow Cytometry: Use 50ul of the suggested working dilution to label 1x10⁶ cells in 100ul

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Rabbit anti Sheep IgG (H/L):Biotin	5184-2304	1.5 ml	C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488	STAR88D488GA	0.1 mg	F IF
Donkey anti Sheep/Goat IgG:FITC	STAR88F	1 mg	C
Donkey anti Sheep/Goat IgG:HRP	STAR88P	1 ml	C E P WB

Product Specific References

References for IgG antibody

Heinrich, F. et al. (2015) Immunophenotyping of immune cell populations in the raccoon (Procyon lotor).
Vet Immunol Immunopathol. 168 (3-4): 140-6.
Tartour, K. et al. (2014) IFITM proteins are incorporated onto HIV-1 virion particles and negatively imprint their infectivity.
Retrovirology. 11: 103.

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