IgG antibody

Sheep anti Rabbit IgG:FITC

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG

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Sheep anti Rabbit IgG recognizes rabbit IgG.

Target Species
Rabbit
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography from serum
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Rabbit IgG.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/50
Immunohistology - Frozen 1/25 1/50
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 106 cells in 100ul.

References for IgG antibody

  1. Auger, G. A. et al. (2004) The use of membrane translocating peptides to identify sites of interaction between the C5a receptor and downstream effector proteins.
    Immunology 112: 590-596.
  2. Rodrigues-Lisoni, F.C. et al. (2006) In vitro and in vivo studies on CCR10 regulation by Annexin A1.
    FEBS Lett. 580: 1431-8.

Flow Cytometry

RRID
AB_321856
UniProt
P01870
Entrez Gene
LOC100009097
GO Terms
GO:0003823 antigen binding
View more products with IGG specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Rabbit Products