IgG antibody

Donkey anti Rabbit IgG (H/L):Alk. Phos. (Human/Rat/Mouse Adsorbed)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG
Region
(H/L)

Product Code Applications Pack Size List Price Your Price Qty
644004
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Donkey anti rabbit IgG (H+L) antibody recognizes rabbit immunoglobulin G heavy and light chains, and shows minimal cross-reactivity with human, rat and mouse serum proteins.
Donkey anti rabbit IgG (H+L) antibody may also recognize the light chains of other rabbit immunoglobulins.

Target Species
Rabbit
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid.
Preparation
Purified IgG prepared by affinity chromatography on Rabbit IgG covalently linked to agarose.
Antiserum Preparation
Antiserum to rabbit IgG was raised by repeated immunisation of donkeys with highly purified antigen.
Buffer Solution
TRIS buffered saline, 1mM MgCl2
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
50% Glycerol
Immunogen
Rabbit IgG.
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2,000 1/4,000
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for IgG antibody

  1. Vega-Flores, G. et al. (2014) The GABAergic septohippocampal pathway is directly involved in internal processes related to operant reward learning.
    Cereb Cortex. 24 (8): 2093-107.
  2. Belostotskaya, G. et al. (2015) Identification of cardiac stem cells within mature cardiac myocytes.
    Cell Cycle. 17 : 1-8.

RRID
AB_619832
UniProt
P01840
P01839
P01838
P01847
P01870
P01841
Entrez Gene
IGKC2
LOC100009097
GO Terms
GO:0003823 antigen binding
View more products with IGG specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Rabbit Products