IgG antibody

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Sheep anti Rabbit IgG:Biotin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
2AB02B C E P WB 1 ml
Sheep anti Rabbit IgG antibody recognizes rabbit IgG (Heavy and light chains). Cross reactivity with bovine, human, mouse or rat serum components has been minimised by adsorption.

Sheep anti Rabbit IgG antibody has been used extensivley as a secondary reagent for the detection of rabbit polyclonal antibodies in immunohistological (Pimanda et al. 2006) and ELISA (Herndon et al. 2010) applications.

Product Details

Target Species
Rabbit
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Mouse
Rat
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Biotin - liquid
Antiserum Preparation
Antisera to rabbit IgG were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Rabbit IgG from pooled sera.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01870 Related reagents
Entrez Gene
LOC100009097 Related reagents
GO Terms
GO:0003823 antigen binding
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/25600 1/51200
Immunohistology - Frozen 1/400 1/800
Immunohistology - Paraffin 1/100 1/200
Western Blotting 1/20000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

Application Based External Images

ELISA

Immunohistology - Paraffin

Product Specific References

References for IgG antibody

  1. Blakey, D.C. et al. (2002) Antitumor activity of the novel vascular targeting agent ZD6126 in a panel of tumor models.
    Clin Cancer Res. 8 (6): 1974-83.
  2. Herndon, C.N. et al. (2010) Differential expression of interleukin-8 by polymorphonuclear leukocytes of two closely related species, Ovis canadensis and Ovis aries, in response to Mannheimia haemolytica infectio
    Infect Immun. 78: 3578-84.
  3. Pimanda, J.E. et al. (2006) Transcriptional link between blood and bone: the stem cell leukemia gene and its +19 stem cell enhancer are active in bone cells.
    Mol Cell Biol. 26: 2615-25.
  4. Dell'Accio, F. et al. (2006) Activation of WNT and BMP signaling in adult human articular cartilage following mechanical injury.
    Arthritis Res Ther. 8: R139.
  5. Clements, K.M. et al. (2011) Matrix metalloproteinase 17 is necessary for cartilage aggrecan degradation in an inflammatory environment.
    Ann Rheum Dis. 70: 683-9.
  6. Saulnier, A. et al. (2013) The Doublesex Homolog Dmrt5 is Required for the Development of the Caudomedial Cerebral Cortex in Mammals.
    Cereb Cortex. 23: 2552-67.
  7. Mitić, T. and McKay, J.S. (2005) Immunohistochemical analysis of acetylation, proliferation, mitosis, and apoptosis in tumor xenografts following administration of a histone deacetylase inhibitor--a pilot study.
    Toxicol Pathol. 33: 792-9.