Sheep anti Rabbit IgG antibody has been used extensivley as a secondary reagent for the detection of rabbit polyclonal antibodies in immunohistological (Pimanda et al. 2006) and ELISA (Herndon et al. 2010) applications.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Human Mouse Rat Bovine
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Antiserum Preparation
- Antisera to rabbit IgG were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin
- Rabbit IgG from pooled sera.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- P01870 Related reagents
- Entrez Gene
- LOC100009097 Related reagents
- GO Terms
- GO:0003823 antigen binding
- For research purposes only
Applications of IgG antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen||1/400||1/800|
|Immunohistology - Paraffin||1/100||1/200|
Product Specific References
References for IgG antibody
Blakey, D.C. et al. (2002) Antitumor activity of the novel vascular targeting agent ZD6126 in a panel of tumor models.
Clin Cancer Res. 8 (6): 1974-83.
Herndon, C.N. et al. (2010) Differential expression of interleukin-8 by polymorphonuclear leukocytes of two closely related species, Ovis canadensis and Ovis aries, in response to Mannheimia haemolytica infectio
Infect Immun. 78: 3578-84.
Pimanda, J.E. et al. (2006) Transcriptional link between blood and bone: the stem cell leukemia gene and its +19 stem cell enhancer are active in bone cells.
Mol Cell Biol. 26: 2615-25.
Dell'Accio, F. et al. (2006) Activation of WNT and BMP signaling in adult human articular cartilage following mechanical injury.
Arthritis Res Ther. 8: R139.
Clements, K.M. et al. (2011) Matrix metalloproteinase 17 is necessary for cartilage aggrecan degradation in an inflammatory environment.
Ann Rheum Dis. 70: 683-9.
Saulnier, A. et al. (2013) The Doublesex Homolog Dmrt5 is Required for the Development of the Caudomedial Cerebral Cortex in Mammals.
Cereb Cortex. 23: 2552-67.
Mitić, T. and McKay, J.S. (2005) Immunohistochemical analysis of acetylation, proliferation, mitosis, and apoptosis in tumor xenografts following administration of a histone deacetylase inhibitor--a pilot study.
Toxicol Pathol. 33: 792-9.