Tubulin antibody

Rabbit anti Tubulin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Tubulin

Product Code Applications Pack Size List Price Your Price Qty
9280-0050G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.5 ml loader
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Search for Batch Specific Datasheets

Rabbit anti Porcine tubulin antibody recognizes tubulin, a protein which is the major constituent of microtubules. Tubulin is a dimer of alpha and beta chains, which binds two molecules of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. There are at least six isotypes of both alpha- and beta- tubulin in human cells, which are distinguished by slightly different amino acid sequences and encoded by a large, multigene family that has been highly conserved throughout evolution.

Although the most important functions of microtubules in proliferative cells are through their actions as components of the mitotic spindle, they are also involved in many other essential functions throughout the cell cycle of both malignant and nonmalignant cells. Antimicrotubule agents including Vinca alkaloids and taxanes may disrupt many of these essential functions.

Target Species
Pig
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to tubulin were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Purified porcine brain tubulin.
Approx. Protein Concentrations
IgG concentration 5.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/50 1/500
Western Blotting 1/250 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for Tubulin antibody

  1. Gelfand, V.I. & Bershadsky, A.D. (1991) Microtubule dynamics: mechanism, regulation, and function.
    Annu Rev Cell Biol. 7: 93-116.
  2. Downing, K.H. & Nogales, E. (1998) Tubulin structure: insights into microtubule properties and functions.
    Curr Opin Struct Biol. 8 (6): 785-91.
  3. Wang, M. & Collins, R.N. (2014) A lysine deacetylase Hos3 is targeted to the bud neck and involved in the spindle position checkpoint.
    Mol Biol Cell. 25 (18): 2720-34.
  4. Coluccio, L.M. et al. (2021) Enhancing Antibodies’ Binding Capacity through Oriented Functionalization of Plasmonic Surfaces
    Nanomaterials. 11 (10): 2620.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

RRID
AB_808758
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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