Tubulin antibody
Rabbit anti Porcine tubulin antibody (9280-0050G) used to fabricate a fubctionalised SPR surface.
Image caption:
Fluorescence recorded without protA, and therefore with anti-tubulin randomly immobilized binding fluorescent tubulin (a left), compared to the surface with protA orienting anti-tubulin binding (a right). The images recorded show an increase in fluorescence recorded of 59% in the surface with the presence of protA. Fluorescent IgG randomly immobilized (b left) compared to fluorescent IgG bound by protA, higher fluorescence recorded when IgG is randomly immobilized, resulting in higher concentration immobilized. Graph (c) show the counts of fluorescent points in relation to their intensity for the a line images. The percentage of immobilized IgG is showed on Graph (d) where the immobilized antibody was calculated by an FI test on the solution used for antibody immobilization.
From: Coluccio ML, Grillo F, Onesto V, Garo V, Scala C, Cuzzola P, Calfa M, Candeloro P, Gentile F, Piletsky S, Malara N.
Enhancing Antibodies’ Binding Capacity through Oriented Functionalization of Plasmonic Surfaces.
Nanomaterials. 2021; 11(10):2620.
doi:10.3390/nano11102620.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti Porcine tubulin antibody recognizes tubulin, a protein which is the major constituent of microtubules. Tubulin is a dimer of alpha and beta chains, which binds two molecules of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. There are at least six isotypes of both alpha- and beta- tubulin in human cells, which are distinguished by slightly different amino acid sequences and encoded by a large, multigene family that has been highly conserved throughout evolution.
Although the most important functions of microtubules in proliferative cells are through their actions as components of the mitotic spindle, they are also involved in many other essential functions throughout the cell cycle of both malignant and nonmalignant cells. Antimicrotubule agents including Vinca alkaloids and taxanes may disrupt many of these essential functions.
Although the most important functions of microtubules in proliferative cells are through their actions as components of the mitotic spindle, they are also involved in many other essential functions throughout the cell cycle of both malignant and nonmalignant cells. Antimicrotubule agents including Vinca alkaloids and taxanes may disrupt many of these essential functions.
Product Details
- Target Species
- Pig
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to tubulin were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Immunogen
- Purified porcine brain tubulin.
- Approx. Protein Concentrations
- IgG concentration 5.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Tubulin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/50 | 1/500 | |
| Western Blotting | 1/250 | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for Tubulin antibody
-
Gelfand, V.I. & Bershadsky, A.D. (1991) Microtubule dynamics: mechanism, regulation, and function.
Annu Rev Cell Biol. 7: 93-116. -
Downing, K.H. & Nogales, E. (1998) Tubulin structure: insights into microtubule properties and functions.
Curr Opin Struct Biol. 8 (6): 785-91. -
Wang, M. & Collins, R.N. (2014) A lysine deacetylase Hos3 is targeted to the bud neck and involved in the spindle position checkpoint.
Mol Biol Cell. 25 (18): 2720-34. -
Coluccio, L.M. et al. (2021) Enhancing Antibodies’ Binding Capacity through Oriented Functionalization of Plasmonic Surfaces
Nanomaterials. 11 (10): 2620.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
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