MCP-1 antibody
Recombinant Pig MCP-1 (PPP016) detected using Rabbit anti Pig MCP-1 (AHP2384) as the capture reagent and biotinylated Rabbit anti Pig MCP-1 (AHP2384B) as the detection reagent followed by Streptavidin:HRP.
Rabbit anti Pig MCP-1 antibody recognizes porcine monocyte chemotactic protein-1 (MCP-1), also known as C-C motif chemokine 2 (CCL2). Porcine MCP-1 is a 76 amino acid secreted protein belonging to the intercrine beta (chemokine CC) family.
The chemokine MCP-1 is expressed by a range of cells including monocytes, vascular endothelial cells, smooth muscle cells, glomerular mesangial cells and osteoblasts.
MCP-1 is thought to play an important role in the inflammatory response of blood monocytes and tissue macrophages.
The chemokine MCP-1 is expressed by a range of cells including monocytes, vascular endothelial cells, smooth muscle cells, glomerular mesangial cells and osteoblasts.
MCP-1 is thought to play an important role in the inflammatory response of blood monocytes and tissue macrophages.
Product Details
- Target Species
- Pig
- Species Cross-Reactivity
-
Target Species Cross Reactivity Horse Dog Bovine Rabbit - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to pig MCP-1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant pig MCP-1
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of despatch
- Shelf Life
- 12 months from date of despatch
More Information
- UniProt
- P42831 Related reagents
- Entrez Gene
- CCL2 Related reagents
- GO Terms
- GO:0005615 extracellular space
- GO:0006954 inflammatory response
- GO:0006955 immune response
- GO:0008009 chemokine activity
- Regulatory
- For research purposes only
Applications of MCP-1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.01ug/ml | 0.5ug/ml | |
| ELISA | 0.5ug/ml | 5ug/ml | |
| Western Blotting | 0.1ug/ml | 2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This product may be used as a detection antibody in a sandwich ELISA together with AHP2384 as the capture reagent. Recombinant pig MCP-1 (PPP016) may be used as a standard.
- ELISA
- This product may be used as a capture antibody in a sandwich ELISA together with AHP2384B as the detection reagent. Recombinant pig MCP-1 (PPP016) may be used as a standard.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
