Insulin antibody

Guinea Pig anti Pig Insulin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Insulin

Product Code Applications Pack Size List Price Your Price Qty
5330-0104G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E P 50 µl
List Price Your Price
Search for Batch Specific Datasheets

Guinea pig anti Pig insulin antibody recognizes porcine insulin, a hormone secreted by the beta cells of the islets of Langerhans in the pancreas. Insulin belongs to a family of hormones including the insulin-like growth hormones and the relaxins. The insulin precursor is a 108 amino acid polypeptide consisting of a 24 aa signal peptide, an insulin A chain, a prohormone polypeptide (also known as the ‘C chain’) and an insulin B chain together having a predicted molecular mass of 11,672 Da. Active insulin migrates with an apparent molecular mass of ~6 kDa in western blotting of porcine, bovine and lapine samples (Schechter et al. 1992).

Insulin is a hormone that functions in carbohydrate homeostasis, and acts to decrease blood glucose concentration by increasing cell permeability to monosaccharides, amino acids and fatty acids, and accelerate glycolysis and glycogen synthesis in the liver, it induces uptake of glucose into cells through its interaction with the glucose transporter GLUT4 (Watson and Pressin 2001). Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumours of B-cell origin such as insulinoma (Schiel et al. 2006).

Guinea pig anti Pig insulin antibody has been successfully used to demonstrate the presence of insulin in the islets of Langerhans of murine pancreas using immunohistochemistry on paraffin embedded material (Ortsäter et al. 2012).

Target Species
Pig
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Native.
Approx. Protein Concentrations
IgG concentration 5.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen 1/50 1/1600
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Pancreas.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Guinea Pig IgG (H/L):HRP AHP863P C E P 1 mg loader
List Price Your Price
loader
Description Goat anti Guinea Pig IgG (H/L):HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
List Price Your Price
Description Antigen Retrieval Buffer, pH8.0

References for Insulin antibody

  1. Ortsäter, H. et al. (2012) Diet supplementation with green tea extract epigallocatechin gallate prevents progression to glucose intolerance in db/db mice.
    Nutr Metab (Lond). 9: 11.
  2. Izumi, K. et al. (2015) Reduced Tyk2 gene expression in β-cells due to natural mutation determines susceptibility to virus-induced diabetes.
    Nat Commun. 6: 6748.
  3. Tian, J. et al. (2019) A Clinically Applicable Positive Allosteric Modulator of GABA Receptors Promotes Human β-Cell Replication and Survival as well as GABA’s Ability to Inhibit Inflammatory T Cells
    J Diabet Res. 2019, Article ID 5783545, 7 pages.

Immunohistology - Paraffin

RRID
AB_1605150
UniProt
P01315
Entrez Gene
INS
GO Terms
GO:0005576 extracellular region
GO:0005179 hormone activity
GO:0006006 glucose metabolic process
GO:0030335 positive regulation of cell migration
GO:0031954 positive regulation of protein autophosphorylation
GO:0045740 positive regulation of DNA replication
GO:0046628 positive regulation of insulin receptor signaling pathway
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
View more products with INSULIN specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Pig Products