Insulin antibody

100% Secure


Guinea Pig anti Pig Insulin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
5330-0104G C E P 50 µl
Guinea pig anti Pig insulin antibody recognizes porcine insulin, a hormone secreted by the beta cells of the islets of Langerhans in the pancreas. Insulin belongs to a family of hormones including the insulin-like growth hormones and the relaxins. The insulin precursor is a 108 amino acid polypeptide consisting of a 24 aa signal peptide, an insulin A chain, a prohormone polypeptide (also known as the ‘C chain’) and an insulin B chain together having a predicted molecular mass of 11,672 Da. Active insulin migrates with an apparent molecular mass of ~6 kDa in western blotting of porcine, bovine and lapine samples (Schechter et al. 1992).

Insulin is a hormone that functions in carbohydrate homeostasis, and acts to decrease blood glucose concentration by increasing cell permeability to monosaccharides, amino acids and fatty acids, and accelerate glycolysis and glycogen synthesis in the liver, it induces uptake of glucose into cells through its interaction with the glucose transporter GLUT4 (Watson and Pressin 2001). Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumours of B-cell origin such as insulinoma (Schiel et al. 2006).

Guinea pig anti Pig insulin antibody has been successfully used to demonstrate the presence of insulin in the islets of Langerhans of murine pancreas using immunohistochemistry on paraffin embedded material (Ortsäter et al. 2012).

Product Details

Target Species
Pig
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to pig insulin were raised by repeated immunisations of Guinea Pigs with highly purified antigen. Ig fraction prepared from whole serum by ammonium sulphate precipitation.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Native.
Approx. Protein Concentrations
IgG concentration 5.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01315 Related reagents
Entrez Gene
INS Related reagents
GO Terms
GO:0005576 extracellular region
GO:0005179 hormone activity
GO:0006006 glucose metabolic process
GO:0030335 positive regulation of cell migration
GO:0031954 positive regulation of protein autophosphorylation
GO:0045740 positive regulation of DNA replication
GO:0046628 positive regulation of insulin receptor signaling pathway
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
Regulatory
For research purposes only

Applications of Insulin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen 1/50 1/1600
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Pancreas.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Guinea Pig IgG (H/L):HRP AHP863P 1 mg C E P

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P

Application Based External Images

Immunohistology - Paraffin

Product Specific References

References for Insulin antibody

  1. Ortsäter, H. et al. (2012) Diet supplementation with green tea extract epigallocatechin gallate prevents progression to glucose intolerance in db/db mice.
    Nutr Metab (Lond). 9: 11.
  2. Izumi, K. et al. (2015) Reduced Tyk2 gene expression in β-cells due to natural mutation determines susceptibility to virus-induced diabetes.
    Nat Commun. 6: 6748.