ULK2 antibody

Rabbit anti Mouse ULK2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
ULK2

Product Code Applications Pack Size List Price Your Price Qty
AAM80
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 mg loader
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loader
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Rabbit anti Mouse ULK2 antibody recognizes mouse Ulk2 (Serine/threonine-protein kinase Ulk2), otherwise known as Unc-51-like kinase 2, a widely expressed mammalian Atg1 homologue and member of the protein kinase superfamily, which shares structural similarity with a serine/threonine kinase found in C. elegans, known to be involved in axonal elongation. Ulk2 and closely-related Ulk1, localize to autophagic isolation membranes under starvation conditions and binding of Atg13 (Autophagy-related protein 13) to Ulk1 and Ulk2, stabilizes and activates them, facilitating the phosphorylation of FIP200. FIP200 is a multifunctional protein involved in cell migration, proliferation, apoptosis, and tumor suppression.

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat Expected from Sequence
Human Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to mouse Ulk2 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide corresponding to amino acids 370-382 of mouse Ulk2. The corresponding sequence differs by two amino acids in rat and three amino acids in human.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 2.5 5.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AAM80 detects a band of approximately 130kDa in HEK-293T cell lysates transfected with mouse ULK2.

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Further Reading

  1. Yan, J. et al. (1999) Mouse ULK2, a novel member of the UNC-51-like protein kinases: unique features of functional domains.
    Oncogene. 18: 5850-9.
  2. Lee, E.J. and Tournier, C. et al. (2011) The requirement of uncoordinated 51-like kinase 1 (ULK1) and ULK2 in the regulation of autophagy.
    Autophagy. 7: 7689-95.

Synonyms
UNC-51-Like Kinase 2
RRID
AB_10845151
UniProt
Q9QY01
Entrez Gene
Ulk2
GO Terms
GO:0004674 protein serine/threonine kinase activity
GO:0007165 signal transduction
GO:0005524 ATP binding
GO:0006468 protein phosphorylation
GO:0048671 negative regulation of collateral sprouting
GO:0048675 axon extension
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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