STAT5A antibody

100% Secure


Rabbit anti STAT5A

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2413 WB 0.1 mg
Rabbit anti STAT5A antibody recognizes signal transducer and activator of transcription 5A (STAT5A), a member of the STAT protein family. STAT5A plays an important role in cell growth, survival, differentiation and transformation by transducing signals from cytokine receptors to the nucleus.

STAT5A is tyrosine phosphorylated by members of the Janus kinase (JAK) family in response to cytokines and growth factor stimulation. Phosphorylation results in STAT activation and the formation of STAT5 homo- and STAT5/STAT3 heterodimers. STAT dimers translocate from the cytoplasm to the nucleus where they act as transcription factors. Deregulated JAK/STAT signaling has been implicated in a variety of cancers, and STATs can regulate the tumor microenvironment to trigger a shift from cancer inhibition to promotion.

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Antiserum Preparation
Antiserum to STAT5A was raised by repeated immunisation of rabbits with highly purified antigen
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
0.2% Gelatin
Immunogen
Peptide mapping to the carboxy terminus of mouse STAT5A
Approx. Protein Concentrations
IgG concentration 0.2 mg/mll

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P42230 Related reagents
Entrez Gene
Stat5a Related reagents
GO Terms
GO:0005515 protein binding
GO:0000255 allantoin metabolic process
GO:0005509 calcium ion binding
GO:0001553 luteinization
GO:0001779 natural killer cell differentiation
GO:0003677 DNA binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0004871 signal transducer activity
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0006101 citrate metabolic process
GO:0006103 2-oxoglutarate metabolic process
GO:0006105 succinate metabolic process
GO:0006107 oxaloacetate metabolic process
GO:0006549 isoleucine metabolic process
GO:0006573 valine metabolic process
GO:0006600 creatine metabolic process
GO:0006631 fatty acid metabolic process
GO:0007565 female pregnancy
GO:0007595 lactation
GO:0018108 peptidyl-tyrosine phosphorylation
GO:0019218 regulation of steroid metabolic process
GO:0019221 cytokine-mediated signaling pathway
GO:0019530 taurine metabolic process
GO:0019915 lipid storage
GO:0030155 regulation of cell adhesion
GO:0030856 regulation of epithelial cell differentiation
GO:0032825 positive regulation of natural killer cell differentiation
GO:0033026 negative regulation of mast cell apoptosis
GO:0033077 T cell differentiation in thymus
GO:0040018 positive regulation of multicellular organism growth
GO:0042104 positive regulation of activated T cell proliferation
GO:0043029 T cell homeostasis
GO:0045086 positive regulation of interleukin-2 biosynthetic process
GO:0045579 positive regulation of B cell differentiation
GO:0045588 positive regulation of gamma-delta T cell differentiation
GO:0045647 negative regulation of erythrocyte differentiation
GO:0045885 positive regulation of survival gene product expression
GO:0045931 positive regulation of mitotic cell cycle
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0045954 positive regulation of natural killer cell mediated cytotoxicity
GO:0046449 creatinine metabolic process
GO:0046543 development of secondary female sexual characteristics
GO:0046544 development of secondary male sexual characteristics
GO:0048541 Peyer's patch development
GO:0050729 positive regulation of inflammatory response
GO:0060376 positive regulation of mast cell differentiation
GO:0060397 JAK-STAT cascade involved in growth hormone signaling pathway
GO:0060740 prostate gland epithelium morphogenesis
GO:0060742 epithelial cell differentiation involved in prostate gland development
GO:0070668 positive regulation of mast cell proliferation
Regulatory
For research purposes only

Applications of STAT5A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti STAT5A recognizes a band of approximately 91 kDa when run under reducing conditions

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*