Rabbit anti STAT3 antibody recognizes signal transducer and activator of transcription 3 (STAT3), a member of the STAT protein family, which plays an important role in cell growth, survival differentiation and transformation, transducing signals from cytokine receptors to the nucleus. STAT3 is phosphorylated my members of the Janus kinase (JAK) family in response to cytokines and growth factors including interleukin-5, interleukin-6, epidermal growth factor and leukaemia inhibitory factor. Phosphorylation results in STAT activation and the formation of STAT3 homo- or STAT3/STAT1 and STAT3/STAT5 heterodimers. STAT dimers translocate from the cytoplasm to the nucleus where they act as transcription factors. Deregulated JAK/STAT signaling has been implicated in a variety of cancers; STATs can regulate the tumor microenvironment and trigger a shift from cancer inhibition to promotion. STAT3 is expressed in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Consistent STAT3 activation has been demonstrated to play a role in cell proliferation and tumor promoting inflammation.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 30% Glycerol
0.5% Bovine Serum Albumin
- Synthetic peptide surrounding amino acid 73 of rat STAT3
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
calcium ion binding
eye photoreceptor cell differentiation
sequence-specific DNA binding transcription factor activity
protein import into nucleus
signal transducer activity
transcription from RNA polymerase II promoter
transcription factor binding
transcription activator activity
cytokine-mediated signaling pathway
protein kinase binding
regulation of multicellular organism growth
sequence-specific DNA binding
positive regulation of transcription from RNA polymerase II promoter
protein dimerization activity
JAK-STAT cascade involved in growth hormone signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of STAT3 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti STAT3 antibody recognizes a band of approximately 97 kDa in Jurkat cell lysate, 3T3 cell lysate and rat kidney tissue lysate
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Secondary Antibodies Available