STAT3 antibody

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Rabbit anti STAT3

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2350 WB 50 µg
Rabbit anti STAT3 antibody recognizes signal transducer and activator of transcription 3 (STAT3), a member of the STAT protein family, which plays an important role in cell growth, survival differentiation and transformation, transducing signals from cytokine receptors to the nucleus. STAT3 is phosphorylated my members of the Janus kinase (JAK) family in response to cytokines and growth factors including interleukin-5, interleukin-6, epidermal growth factor and leukaemia inhibitory factor. Phosphorylation results in STAT activation and the formation of STAT3 homo- or STAT3/STAT1 and STAT3/STAT5 heterodimers. STAT dimers translocate from the cytoplasm to the nucleus where they act as transcription factors. Deregulated JAK/STAT signaling has been implicated in a variety of cancers; STATs can regulate the tumor microenvironment and trigger a shift from cancer inhibition to promotion. STAT3 is expressed in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Consistent STAT3 activation has been demonstrated to play a role in cell proliferation and tumor promoting inflammation.

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
30% Glycerol
0.5% Bovine Serum Albumin
0.01% Thiomersal
Immunogen
Synthetic peptide surrounding amino acid 73 of rat STAT3
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P42227 Related reagents
Entrez Gene
Stat3 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005509 calcium ion binding
GO:0001659 temperature homeostasis
GO:0001754 eye photoreceptor cell differentiation
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0006953 acute-phase response
GO:0006606 protein import into nucleus
GO:0005829 cytosol
GO:0004871 signal transducer activity
GO:0005634 nucleus
GO:0006366 transcription from RNA polymerase II promoter
GO:0008134 transcription factor binding
GO:0016563 transcription activator activity
GO:0019221 cytokine-mediated signaling pathway
GO:0019901 protein kinase binding
GO:0019953 sexual reproduction
GO:0040014 regulation of multicellular organism growth
GO:0042593 glucose homeostasis
GO:0042755 eating behavior
GO:0043565 sequence-specific DNA binding
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0046983 protein dimerization activity
GO:0060397 JAK-STAT cascade involved in growth hormone signaling pathway
Regulatory
For research purposes only

Applications of STAT3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 0.5 4.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti STAT3 antibody recognizes a band of approximately 97 kDa in Jurkat cell lysate, 3T3 cell lysate and rat kidney tissue lysate

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB