SF-20 antibody

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Rabbit anti Mouse SF-20:Biotin

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: SF-20

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Rabbit anti Mouse SF-20 antibody recognizes mouse stromal cell-derived growth factor 20 (SF-20), otherwise known as interleukin-25 (IL-25), a 166 amino acid secreted growth factor and member of the UPF0556 family.

SF-20 should not be confused with interleukin 17E (IL-17E), which is also alternatively called IL-25.

Target Species: Mouse
Product Form: Purified IgG conjugated to Biotin - lyophilized
Reconstitution: Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
NB: For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation: Antisera to mouse SF-20 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: None present
Carrier Free: Yes
Immunogen: Recombinant mouse SF-20.
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		0.25	1.0ug/ml
Western Blotting		0.1	0.2ug/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Synonyms: Interleukin-25
RRID: AB_2124934
UniProt: Q9CPT4
Entrez Gene: D17Wsu104e
GO Terms: GO:0005576 extracellular region