RANTES antibody

Rabbit anti Mouse RANTES

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
RANTES

Product Code Applications Pack Size List Price Your Price Qty
AAM49
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN P WB 0.1 mg loader
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loader
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Rabbit anti Mouse RANTES antibody recognizes mouse RANTES, also known as CCL5.

RANTES is expressed by circulating T cells and T cells in culture. RANTES is chemotactic for peripheral blood monocytes.

Target Species
Mouse
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Antiserum Preparation
Antisera to mouse RANTES were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant mouse RANTES.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5ug/ml
Functional Assays 6.0ug/ml 8.0ug/ml
Immunohistology - Paraffin
Western Blotting 0.1ug/ml 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for RANTES antibody

  1. Ruddell, R.G. et al. (2009) Lymphotoxin-beta receptor signaling regulates hepatic stellate cell function and wound healing in a murine model of chronic liver injury.
    Hepatology. 49: 227-39.
  2. Keepers, T.R. et al. (2007) Monocyte chemoattractant protein 1, macrophage inflammatory protein 1 alpha, and RANTES recruit macrophages to the kidney in a mouse model of hemolytic-uremic syndrome.
    Infect Immun. 75: 1229-36.
  3. Hildebrandt, G.C. et al. (2005) Donor T-cell production of RANTES significantly contributes to the development of idiopathic pneumonia syndrome after allogeneic stem cell transplantation.
    Blood. 105: 2249-57.

Immunohistology - Paraffin

Synonyms
CCL5
RRID
AB_2275384
UniProt
P30882
Entrez Gene
Ccl5
GO Terms
GO:0005615 extracellular space
GO:0005737 cytoplasm
GO:0006955 immune response
GO:0034612 response to tumor necrosis factor
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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