Osteocalcin antibody

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Goat anti Mouse Osteocalcin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
7060-1815 C IF 0.1 ml
Goat anti mouse osteocalcin antibody is specific for intact osteocalcin and recognizes a site at the carboxyl terminal.

Osteocalcin (Bone Gla Protein) is a 49 amino acid single chain vitamin K dependent protein (MW 5.8 kDa), made by osteoblasts that is a major component of the non-collagenous bone matrix. Serum osteocalcin is elevated in diseases characterized by increased bone turnover such as osteoporosis, hyperparathyroidism and Paget’s disease, and low in conditions associated with low bone turnover such as hypoparathyroidism and growth hormone deficiency.

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Human
Bovine
Monkey
Elk
Chicken
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Preservative Stabilisers
0.1%Sodium Azide (NaN3)
Immunogen
Highly purified native mouse osteocalcin.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P86547 Related reagents
Entrez Gene
Bglap2 Related reagents
GO Terms
GO:0005509 calcium ion binding
GO:0005615 extracellular space
GO:0030500 regulation of bone mineralization
GO:0031016 pancreas development
GO:0031214 biomineral tissue development
GO:0032024 positive regulation of insulin secretion
GO:0032869 cellular response to insulin stimulus
GO:0042593 glucose homeostasis
GO:0044242 cellular lipid catabolic process
GO:0044342 type B pancreatic cell proliferation
GO:0046848 hydroxyapatite binding
Regulatory
For research purposes only

Applications of Osteocalcin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Frozen 1/50 1/500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Application Based External Images

Immunofluorescence

Product Specific References

References for Osteocalcin antibody

  1. Gundberg, C. M. et al. (1992) Development and validation of a radioimmunoassay for mouse osteocalcin: paradoxical response in the Hyp mouse.
    Endocrinology.130 (4):1909-1915.
  2. Matsumoto, T. et al. (2010) Lnk-dependent axis of SCF-cKit signal for osteogenesis in bone fracture healing.
    J Exp Med. 207: 2207-23.
  3. Davies, M.R. et al. (2003) BMP-7 is an efficacious treatment of vascular calcification in a murine model of atherosclerosis and chronic renal failure.
    J Am Soc Nephrol. 14 (6): 1559-67.
  4. Tasso, R. et al. (2009) Recruitment of a host's osteoprogenitor cells using exogenous mesenchymal stem cells seeded on porous ceramic.
    Tissue Eng Part A. 15: 2203-12.
  5. Qian, H. et al. (2013) Molecular characterization of prospectively isolated multipotent mesenchymal progenitors provides new insight into the cellular identity of mesenchymal stem cells in mouse bone marrow.
    Mol Cell Biol. 33: 661-77.
  6. Cherry, H.M. et al. (2014) In vivo phenotypic characterisation of nucleoside label-retaining cells in mouse periosteum.
    Eur Cell Mater. 27: 185-95; discussion 195.
  7. Ma, M.S. et al. (2016) Characterization and comparison of osteoblasts derived from mouse embryonic stem cells and induced pluripotent stem cells.
    J Bone Miner Metab. Jan 8. [Epub ahead of print]
  8. Sista S et al. (2011) The influence of surface energy of titanium-zirconium alloy on osteoblast cell functions in vitro.
    J Biomed Mater Res A. 97 (1): 27-36.