Noxa antibody
Rabbit anti Murine Noxa antibody recognises murine Noxa, an ~11 kDa member of the BH3-only proapoptotic protein family. Noxa is localized to the mitochondria and is a transcriptional target of p73, p53 and BH3-only proteins. It is thought to induce apoptosis when activated, lowering the activity of anti-apoptotic Bcl-2-like proteins. It can be upregulated by mitogenic activation, independently of p53. In humans, along with Mcl-1 it is thought to form a selective pathway that restrains lymphocyte proliferation.
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to NOXA were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide (NaN3) - Immunogen
- Synthetic peptide corresponding to N-terminus of mouse NOXA.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9JM54
- Entrez Gene
- Pmaip1
- GO Terms
- GO:0005515 protein binding
- GO:0001836 release of cytochrome c from mitochondria
- GO:0001844 protein insertion into mitochondrial membrane involved in induction of apoptosis
- GO:0005739 mitochondrion
- GO:0006974 response to DNA damage stimulus
- GO:0008629 induction of apoptosis by intracellular signals
- GO:0009411 response to UV
- GO:0010165 response to X-ray
- GO:0043525 positive regulation of neuron apoptosis
- GO:0048147 negative regulation of fibroblast proliferation
- Regulatory
- For research purposes only
Applications of Noxa antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 | |||
| Western Blotting | 0.5 | 1.0ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1016 detects a band of approximately 11kDa in human stomach cell lysates.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for Noxa antibody
-
Ikezoe, T. et al. (2010) p53 is critical for the Aurora B kinase inhibitor-mediated apoptosis in acute myelogenous leukemia cells.
Int J Hematol. 91 (1): 69-77. -
Yu, S.J. et al. (2018) MicroRNA-200a confers chemoresistance by antagonizing TP53INP1 and YAP1 in human breast cancer.
BMC Cancer. 18 (1): 74.
Further Reading
-
Oda, E. et al. (2000) Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis.
Science. 288 (5468): 1053-8. -
Michels, J. et al. (2005) Mcl-1.
Int J Biochem Cell Biol. 37 (2): 267-71.
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