Noxa antibody

Rabbit anti Noxa

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Noxa

Product Code Applications Pack Size List Price Your Price Qty
AHP1016
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SDS Safety Datasheet SDS
P * WB 0.1 mg loader
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Rabbit anti Murine Noxa antibody recognises murine Noxa, an ~11 kDa member of the BH3-only proapoptotic protein family. Noxa is localized to the mitochondria and is a transcriptional target of p73, p53 and BH3-only proteins. It is thought to induce apoptosis when activated, lowering the activity of anti-apoptotic Bcl-2-like proteins. It can be upregulated by mitogenic activation, independently of p53. In humans, along with Mcl-1 it is thought to form a selective pathway that restrains lymphocyte proliferation.

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to NOXA were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to N-terminus of mouse NOXA.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1
Western Blotting 0.5 1.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1016 detects a band of approximately 11kDa in human stomach cell lysates.

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References for Noxa antibody

  1. Ikezoe, T. et al. (2010) p53 is critical for the Aurora B kinase inhibitor-mediated apoptosis in acute myelogenous leukemia cells.
    Int J Hematol. 91 (1): 69-77.
  2. Yu, S.J. et al. (2018) MicroRNA-200a confers chemoresistance by antagonizing TP53INP1 and YAP1 in human breast cancer.
    BMC Cancer. 18 (1): 74.

Further Reading

  1. Oda, E. et al. (2000) Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis.
    Science. 288 (5468): 1053-8.
  2. Michels, J. et al. (2005) Mcl-1.
    Int J Biochem Cell Biol. 37 (2): 267-71.

Synonyms
PMAIP1
RRID
AB_2252610
UniProt
Q9JM54
Entrez Gene
Pmaip1
GO Terms
GO:0005515 protein binding
GO:0001836 release of cytochrome c from mitochondria
GO:0001844 protein insertion into mitochondrial membrane involved in induction of apoptosis
GO:0005739 mitochondrion
GO:0006974 response to DNA damage stimulus
GO:0008629 induction of apoptosis by intracellular signals
GO:0009411 response to UV
GO:0010165 response to X-ray
GO:0043525 positive regulation of neuron apoptosis
GO:0048147 negative regulation of fibroblast proliferation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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