Rabbit anti inducible nitric oxide synthase antibody recognizes inducible nitric oxide sythase (iNOS), also known as Macrophage NOS, NOS type II or Peptidyl-cysteine S-nitrosylase NOS2. Rabbit anti inducible nitric oxide synthase antibody does not cross react with endothelial nitric oxide synthase (eNOS) or brain nitric oxide synthase (bNOS).
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to inducible Nitric Oxide Synthase were raised by repeated immunisation of rabbits with highly purified antigen. Whole serum is supplied.
- Preservative Stabilisers
- 0.05% Sodium Azide (NaN3)
- Mouse macrophage NOS C-terminal peptide (1131-1144) + additional N-terminal Cys conjugated to KLH (CKKGSALEEPKATRL).
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
response to hypoxia
nitric-oxide synthase activity
arginine catabolic process
superoxide metabolic process
nitric oxide biosynthetic process
regulation of cell proliferation
protein homodimerization activity
perinuclear region of cytoplasm
flavin adenine dinucleotide binding
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of NOS-II antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
References for NOS-II antibody
Lyons, C.R. et al. (1992) Molecular cloning and functional expression of an inducible nitric oxide synthase from a murine macrophage cell line.
J Biol Chem. 267 (9): 6370-4.
Lowenstein, C.J. et al. (1992) Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme.
Proc Natl Acad Sci U S A. 89 (15): 6711-5.
Bredt, D.S. et al. (1991) Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase.
Nature. 351 (6329): 714-8.
Hohenstein, B. et al. (2007) Analysis of NO-synthase expression and clinical risk factors in human diabetic
Nephrol Dial Transplant. 23: 1346-54.
Fenoglio, C. et al. (2004) Expression of natriuretic peptides, nitric oxide synthase, and guanylate cyclase activity in frog mesonephros during the annual cycle.
Gen Comp Endocrinol. 137: 166-76.
Elmann, A. et al. (2011) Anti-neuroinflammatory effects of the extract of Achillea fragrantissima.
BMC Complement Altern Med. 11: 98.
Montes de Oca, M. et al. (2008) Peripheral muscle alterations in non-COPD smokers.
Chest. 133 (1): 13-8.
Macura, S.L. et al. (2013) Microspheres targeted with a mesothelin antibody and loaded with doxorubicin reduce tumor volume of human mesotheliomas in xenografts.
BMC Cancer. 13: 400.
Subhan, F. et al. (2017) Fish Scale Collagen Peptides Protect against CoCl2/TNF-α-Induced Cytotoxicity and Inflammation via Inhibition of ROS, MAPK, and NF-κB Pathways in HaCaT Cells.
Oxid Med Cell Longev. 2017: 9703609.