NOS-II antibody

100% Secure

Anti Inducible Nitric Oxide Synthase Antibody thumbnail image 1

Anti Inducible Nitric Oxide Synthase Antibody thumbnail image 2

Anti Inducible Nitric Oxide Synthase Antibody gallery image 1 — **Published customer image:**
Rabbit anti Human nitric oxide synthase antibody (**AHP303**) used for the detection of iNOS in stimulated microglial lysates by western blotting.
**Image caption:**
Inhibition of iNOS and COX-2 expression in LPS-stimulated microglial cells by Af extract. Microglial cells were treated with 100 μg/mL of Af extract, followed by stimulation with LPS for 20 h. Equal amounts of cell lysates were separated by SDS-PAGE and immunoblotted with antibodies to iNOS, COX-2, and β-actin. A. The immunoblot represents one of three different experiments with similar results. B. Data represent the means ± SEM of three independent experiments (n = 3). The levels of each protein were normalized to the levels of β-actin protein.

From: Elmann A, Mordechay S, Erlank H, Telerman A, Rindner M, Ofir R.
Anti-neuroinflammatory effects of the extract of *Achillea fragrantissima*.
BMC Complement Altern Med. 2011 Oct 21;11:98.

Anti Inducible Nitric Oxide Synthase Antibody gallery image 2 — **Published customer image:**
Rabbit anti iNOS antibody (**AHP303**) used to assess iNOS expression levels in HaCaT cells by western blotting.
**Image caption:**
Inhibitory effects of FSCP on CoCl2-induced activation of p38/MAPK signaling pathway.
Western blot analysis showed that the treatment of HaCaT cells with FSCP and SB203580 (SB) suppressed CoCl2-induced elevated expression of iNOS and Bax.Results are presented as the means ± SD of three independent experiments. #p <0.05 versus the control and ∗p <0.05, ∗∗p <0.01, and ∗∗∗p <0.001 versus the CoCl₂-treated group.

From: Subhan F, Kang HY, Lim Y, Ikram M, Baek SY, Jin S, Jeong YH, Kwak JY, Yoon S.
Fish Scale Collagen Peptides Protect against CoCl₂/TNF-α-Induced Cytotoxicity and Inflammation via Inhibition of ROS, MAPK, and NF-κB Pathways in HaCaT Cells.
Oxid Med Cell Longev. 2017; 2017:9703609.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.

Rabbit anti Inducible Nitric Oxide Synthase

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
AHP303 Datasheet	C WB SDS	0.1 ml	Log in		Get Quote

Rabbit anti inducible nitric oxide synthase antibody recognizes inducible nitric oxide sythase (iNOS), also known as Macrophage NOS, NOS type II or Peptidyl-cysteine S-nitrosylase NOS2. Rabbit anti inducible nitric oxide synthase antibody does not cross react with endothelial nitric oxide synthase (eNOS) or brain nitric oxide synthase (bNOS).

Product Details

Target Species

Mouse

Species Cross-Reactivity

Target Species	Cross Reactivity
Rat
Human

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Serum - liquid

Antiserum Preparation

Antisera to inducible Nitric Oxide Synthase were raised by repeated immunisation of rabbits with highly purified antigen. Whole serum is supplied.

Preservative Stabilisers

0.05% Sodium Azide (NaN₃)

Immunogen

Mouse macrophage NOS C-terminal peptide (1131-1144) + additional N-terminal Cys conjugated to KLH (CKKGSALEEPKATRL).

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee: 12 months from date of despatch

More Information

UniProt: P29477
Entrez Gene: Nos2
GO Terms: GO:0001666 response to hypoxia; GO:0004517 nitric-oxide synthase activity; GO:0005829 cytosol; GO:0005516 calmodulin binding; GO:0055114 oxidation-reduction process; GO:0006527 arginine catabolic process; GO:0006801 superoxide metabolic process; GO:0006809 nitric oxide biosynthetic process; GO:0006954 inflammatory response; GO:0010181 FMN binding; GO:0018119 peptidyl-cysteine S-nitrosylation; GO:0020037 heme binding; GO:0030863 cortical cytoskeleton; GO:0034617 tetrahydrobiopterin binding; GO:0034618 arginine binding; GO:0042127 regulation of cell proliferation; GO:0042803 protein homodimerization activity; GO:0048471 perinuclear region of cytoplasm; GO:0050660 flavin adenine dinucleotide binding; GO:0050661 NADP binding
Regulatory: For research purposes only

Applications of NOS-II antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Frozen			1/500
Western Blotting			1/2000

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	E WB	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (Fc):FITC	STAR121F	F	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (Fc):HRP	STAR121P	E WB	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (H/L):HRP	STAR124P	C E WB	1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:FITC	STAR34B	C F	1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:RPE	STAR35A	F	1 ml	Log in	Get Quote
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	F IF WB	0.1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	F IF WB	0.1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	F IF WB	0.1 mg	Log in	Get Quote

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	WB*	0.5 ml	Log in		Get Quote

Product Specific References

References for NOS-II antibody

Lyons, C.R. et al. (1992) Molecular cloning and functional expression of an inducible nitric oxide synthase from a murine macrophage cell line.
J Biol Chem. 267 (9): 6370-4.
Lowenstein, C.J. et al. (1992) Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme.
Proc Natl Acad Sci U S A. 89 (15): 6711-5.
Bredt, D.S. et al. (1991) Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase.
Nature. 351 (6329): 714-8.
Hohenstein, B. et al. (2007) Analysis of NO-synthase expression and clinical risk factors in human diabetic nephropathy.
Nephrol Dial Transplant. 23: 1346-54.
Fenoglio, C. et al. (2004) Expression of natriuretic peptides, nitric oxide synthase, and guanylate cyclase activity in frog mesonephros during the annual cycle.
Gen Comp Endocrinol. 137: 166-76.
Elmann, A. et al. (2011) Anti-neuroinflammatory effects of the extract of Achillea fragrantissima.
BMC Complement Altern Med. 11: 98.
Montes de Oca, M. et al. (2008) Peripheral muscle alterations in non-COPD smokers.
Chest. 133 (1): 13-8.
Macura, S.L. et al. (2013) Microspheres targeted with a mesothelin antibody and loaded with doxorubicin reduce tumor volume of human mesotheliomas in xenografts.
BMC Cancer. 13: 400.
Subhan, F. et al. (2017) Fish Scale Collagen Peptides Protect against CoCl₂/TNF-α-Induced Cytotoxicity and Inflammation via Inhibition of ROS, MAPK, and NF-κB Pathways in HaCaT Cells.
Oxid Med Cell Longev. 2017: 9703609.

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer