NOS-II antibody
Rabbit anti Human nitric oxide synthase antibody (AHP303) used for the detection of iNOS in stimulated microglial lysates by western blotting.
Image caption:
Inhibition of iNOS and COX-2 expression in LPS-stimulated microglial cells by Af extract. Microglial cells were treated with 100 μg/mL of Af extract, followed by stimulation with LPS for 20 h. Equal amounts of cell lysates were separated by SDS-PAGE and immunoblotted with antibodies to iNOS, COX-2, and β-actin. A. The immunoblot represents one of three different experiments with similar results. B. Data represent the means ± SEM of three independent experiments (n = 3). The levels of each protein were normalized to the levels of β-actin protein.
From: Elmann A, Mordechay S, Erlank H, Telerman A, Rindner M, Ofir R.
Anti-neuroinflammatory effects of the extract of Achillea fragrantissima.
BMC Complement Altern Med. 2011 Oct 21;11:98.
Rabbit anti iNOS antibody (AHP303) used to assess iNOS expression levels in HaCaT cells by western blotting.
Image caption:
Inhibitory effects of FSCP on CoCl2-induced activation of p38/MAPK signaling pathway.
Western blot analysis showed that the treatment of HaCaT cells with FSCP and SB203580 (SB) suppressed CoCl2-induced elevated expression of iNOS and Bax.Results are presented as the means ± SD of three independent experiments. #p <0.05 versus the control and ∗p <0.05, ∗∗p <0.01, and ∗∗∗p <0.001 versus the CoCl2-treated group.
From: Subhan F, Kang HY, Lim Y, Ikram M, Baek SY, Jin S, Jeong YH, Kwak JY, Yoon S.
Fish Scale Collagen Peptides Protect against CoCl2/TNF-α-Induced Cytotoxicity and Inflammation via Inhibition of ROS, MAPK, and NF-κB Pathways in HaCaT Cells.
Oxid Med Cell Longev. 2017; 2017:9703609.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rabbit anti inducible nitric oxide synthase antibody recognizes inducible nitric oxide sythase (iNOS), also known as Macrophage NOS, NOS type II or Peptidyl-cysteine S-nitrosylase NOS2. Rabbit anti inducible nitric oxide synthase antibody does not cross react with endothelial nitric oxide synthase (eNOS) or brain nitric oxide synthase (bNOS).
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to inducible Nitric Oxide Synthase were raised by repeated immunisation of rabbits with highly purified antigen. Whole serum is supplied.
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Mouse macrophage NOS C-terminal peptide (1131-1144) + additional N-terminal Cys conjugated to KLH (CKKGSALEEPKATRL).
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P29477
- Entrez Gene
- Nos2
- GO Terms
- GO:0001666 response to hypoxia
- GO:0004517 nitric-oxide synthase activity
- GO:0005829 cytosol
- GO:0005516 calmodulin binding
- GO:0055114 oxidation-reduction process
- GO:0006527 arginine catabolic process
- GO:0006801 superoxide metabolic process
- GO:0006809 nitric oxide biosynthetic process
- GO:0006954 inflammatory response
- GO:0010181 FMN binding
- GO:0018119 peptidyl-cysteine S-nitrosylation
- GO:0020037 heme binding
- GO:0030863 cortical cytoskeleton
- GO:0034617 tetrahydrobiopterin binding
- GO:0034618 arginine binding
- GO:0042127 regulation of cell proliferation
- GO:0042803 protein homodimerization activity
- GO:0048471 perinuclear region of cytoplasm
- GO:0050660 flavin adenine dinucleotide binding
- GO:0050661 NADP binding
- Regulatory
- For research purposes only
Applications of NOS-II antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | 1/500 | ||
| Western Blotting | 1/2000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
|
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for NOS-II antibody
-
Lyons, C.R. et al. (1992) Molecular cloning and functional expression of an inducible nitric oxide synthase from a murine macrophage cell line.
J Biol Chem. 267 (9): 6370-4. -
Lowenstein, C.J. et al. (1992) Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme.
Proc Natl Acad Sci U S A. 89 (15): 6711-5. -
Bredt, D.S. et al. (1991) Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase.
Nature. 351 (6329): 714-8. -
Hohenstein, B. et al. (2007) Analysis of NO-synthase expression and clinical risk factors in human diabetic
nephropathy.
Nephrol Dial Transplant. 23: 1346-54. -
Fenoglio, C. et al. (2004) Expression of natriuretic peptides, nitric oxide synthase, and guanylate cyclase activity in frog mesonephros during the annual cycle.
Gen Comp Endocrinol. 137: 166-76. -
Elmann, A. et al. (2011) Anti-neuroinflammatory effects of the extract of Achillea fragrantissima.
BMC Complement Altern Med. 11: 98. -
Montes de Oca, M. et al. (2008) Peripheral muscle alterations in non-COPD smokers.
Chest. 133 (1): 13-8. -
Macura, S.L. et al. (2013) Microspheres targeted with a mesothelin antibody and loaded with doxorubicin reduce tumor volume of human mesotheliomas in xenografts.
BMC Cancer. 13: 400. -
Subhan, F. et al. (2017) Fish Scale Collagen Peptides Protect against CoCl2/TNF-α-Induced Cytotoxicity and Inflammation via Inhibition of ROS, MAPK, and NF-κB Pathways in HaCaT Cells.
Oxid Med Cell Longev. 2017: 9703609.
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