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NOS-II antibody

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Anti Inducible Nitric Oxide Synthase Antibody thumbnail image 1 Anti Inducible Nitric Oxide Synthase Antibody thumbnail image 2
Anti Inducible Nitric Oxide Synthase Antibody gallery image 1
Published customer image:
Rabbit anti Human nitric oxide synthase antibody (AHP303) used for the detection of iNOS in stimulated microglial lysates by western blotting.
Image caption:
Inhibition of iNOS and COX-2 expression in LPS-stimulated microglial cells by Af extract. Microglial cells were treated with 100 μg/mL of Af extract, followed by stimulation with LPS for 20 h. Equal amounts of cell lysates were separated by SDS-PAGE and immunoblotted with antibodies to iNOS, COX-2, and β-actin. A. The immunoblot represents one of three different experiments with similar results. B. Data represent the means ± SEM of three independent experiments (n = 3). The levels of each protein were normalized to the levels of β-actin protein.

From: Elmann A, Mordechay S, Erlank H, Telerman A, Rindner M, Ofir R.
Anti-neuroinflammatory effects of the extract of Achillea fragrantissima.
BMC Complement Altern Med. 2011 Oct 21;11:98.
Anti Inducible Nitric Oxide Synthase Antibody gallery image 2
Published customer image:
Rabbit anti iNOS antibody (AHP303) used to assess iNOS expression levels in HaCaT cells by western blotting.
Image caption:
Inhibitory effects of FSCP on CoCl2-induced activation of p38/MAPK signaling pathway.
Western blot analysis showed that the treatment of HaCaT cells with FSCP and SB203580 (SB) suppressed CoCl2-induced elevated expression of iNOS and Bax.Results are presented as the means ± SD of three independent experiments. #p <0.05 versus the control and ∗p <0.05, ∗∗p <0.01, and ∗∗∗p <0.001 versus the CoCl2-treated group.

From: Subhan F, Kang HY, Lim Y, Ikram M, Baek SY, Jin S, Jeong YH, Kwak JY, Yoon S.
Fish Scale Collagen Peptides Protect against CoCl2/TNF-α-Induced Cytotoxicity and Inflammation via Inhibition of ROS, MAPK, and NF-κB Pathways in HaCaT Cells.
Oxid Med Cell Longev. 2017; 2017:9703609.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.

Rabbit anti Inducible Nitric Oxide Synthase

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP303 C WB 0.1 ml
Rabbit anti inducible nitric oxide synthase antibody recognizes inducible nitric oxide sythase (iNOS), also known as Macrophage NOS, NOS type II or Peptidyl-cysteine S-nitrosylase NOS2. Rabbit anti inducible nitric oxide synthase antibody does not cross react with endothelial nitric oxide synthase (eNOS) or brain nitric oxide synthase (bNOS).

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Antiserum Preparation
Antisera to inducible Nitric Oxide Synthase were raised by repeated immunisation of rabbits with highly purified antigen. Whole serum is supplied.
Preservative Stabilisers
0.05% Sodium Azide (NaN3)
Immunogen
Mouse macrophage NOS C-terminal peptide (1131-1144) + additional N-terminal Cys conjugated to KLH (CKKGSALEEPKATRL).

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P29477 Related reagents
Entrez Gene
Nos2 Related reagents
GO Terms
GO:0001666 response to hypoxia
GO:0004517 nitric-oxide synthase activity
GO:0005829 cytosol
GO:0005516 calmodulin binding
GO:0055114 oxidation-reduction process
GO:0006527 arginine catabolic process
GO:0006801 superoxide metabolic process
GO:0006809 nitric oxide biosynthetic process
GO:0006954 inflammatory response
GO:0010181 FMN binding
GO:0018119 peptidyl-cysteine S-nitrosylation
GO:0020037 heme binding
GO:0030863 cortical cytoskeleton
GO:0034617 tetrahydrobiopterin binding
GO:0034618 arginine binding
GO:0042127 regulation of cell proliferation
GO:0042803 protein homodimerization activity
GO:0048471 perinuclear region of cytoplasm
GO:0050660 flavin adenine dinucleotide binding
GO:0050661 NADP binding
Regulatory
For research purposes only

Applications of NOS-II antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/500
Western Blotting 1/2000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

References for NOS-II antibody

  1. Lyons, C.R. et al. (1992) Molecular cloning and functional expression of an inducible nitric oxide synthase from a murine macrophage cell line.
    J Biol Chem. 267 (9): 6370-4.
  2. Lowenstein, C.J. et al. (1992) Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme.
    Proc Natl Acad Sci U S A. 89 (15): 6711-5.
  3. Bredt, D.S. et al. (1991) Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase.
    Nature. 351 (6329): 714-8.
  4. Hohenstein, B. et al. (2007) Analysis of NO-synthase expression and clinical risk factors in human diabetic nephropathy.
    Nephrol Dial Transplant. 23: 1346-54.
  5. Fenoglio, C. et al. (2004) Expression of natriuretic peptides, nitric oxide synthase, and guanylate cyclase activity in frog mesonephros during the annual cycle.
    Gen Comp Endocrinol. 137: 166-76.
  6. Elmann, A. et al. (2011) Anti-neuroinflammatory effects of the extract of Achillea fragrantissima.
    BMC Complement Altern Med. 11: 98.
  7. Montes de Oca, M. et al. (2008) Peripheral muscle alterations in non-COPD smokers.
    Chest. 133 (1): 13-8.
  8. Macura, S.L. et al. (2013) Microspheres targeted with a mesothelin antibody and loaded with doxorubicin reduce tumor volume of human mesotheliomas in xenografts.
    BMC Cancer. 13: 400.
  9. Subhan, F. et al. (2017) Fish Scale Collagen Peptides Protect against CoCl2/TNF-α-Induced Cytotoxicity and Inflammation via Inhibition of ROS, MAPK, and NF-κB Pathways in HaCaT Cells.
    Oxid Med Cell Longev. 2017: 9703609.
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