Rabbit anti nNOS antibody
recognizes neuronal nitric oxide synthase also known as nNOS and NOS type I. nNOS catalyzes the synthesis of the second messenger molecule and neurotransmitter, nitric oxide (NO), in the brain. NO is also produced by immune cells, such as macrophages, as part of the immune response by the iNOS (inducible nitric oxide synthase). In endothelia, the molecule is produced by eNOS (endothelial nitric oxide synthase) where it acts as a vasodilator. Neuronal nitrogen oxide synthase, via regulation of NO levels, is important for nervous system development and plays a role in development and learning (Esplugues, 2002
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to nNOS was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Synthetic peptide corresponding to the sequence near the C-terminus of nNOS
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch.
- Entrez Gene
- GO Terms
regulation of sodium ion transport
nitric-oxide synthase activity
negative regulation of potassium ion transport
nitric oxide biosynthetic process
striated muscle contraction
positive regulation of histone acetylation
exogenous drug catabolic process
positive regulation of transcription, DNA-dependent
flavin adenine dinucleotide binding
negative regulation of hydrolase activity
negative regulation of serotonin uptake
negative regulation of calcium ion transport
cellular response to growth factor stimulus
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of nNOS antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti nNOS recognizes a band of ~155 kDa by Western blot
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Secondary Antibodies Available
Useful Reagents Available