nNOS antibody
Rabbit anti nNOS antibody recognizes neuronal nitric oxide synthase also known as nNOS and NOS type I. nNOS catalyzes the synthesis of the second messenger molecule and neurotransmitter, nitric oxide (NO), in the brain. NO is also produced by immune cells, such as macrophages, as part of the immune response by the iNOS (inducible nitric oxide synthase). In endothelia, the molecule is produced by eNOS (endothelial nitric oxide synthase) where it acts as a vasodilator. Neuronal nitrogen oxide synthase, via regulation of NO levels, is important for nervous system development and plays a role in development and learning (Esplugues, 2002).
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to nNOS was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
50% Glycerol - Immunogen
- Synthetic peptide corresponding to the sequence near the C-terminus of nNOS
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9Z0J4
- Entrez Gene
- Nos1
- GO Terms
- GO:0002028 regulation of sodium ion transport
- GO:0004517 nitric-oxide synthase activity
- GO:0043267 negative regulation of potassium ion transport
- GO:0005516 calmodulin binding
- GO:0005856 cytoskeleton
- GO:0055114 oxidation-reduction process
- GO:0006809 nitric oxide biosynthetic process
- GO:0006941 striated muscle contraction
- GO:0010181 FMN binding
- GO:0018119 peptidyl-cysteine S-nitrosylation
- GO:0020037 heme binding
- GO:0035066 positive regulation of histone acetylation
- GO:0042383 sarcolemma
- GO:0042738 exogenous drug catabolic process
- GO:0043197 dendritic spine
- GO:0045202 synapse
- GO:0045893 positive regulation of transcription, DNA-dependent
- GO:0050660 flavin adenine dinucleotide binding
- GO:0050661 NADP binding
- GO:0051346 negative regulation of hydrolase activity
- GO:0051612 negative regulation of serotonin uptake
- GO:0051926 negative regulation of calcium ion transport
- GO:0071363 cellular response to growth factor stimulus
- Regulatory
- For research purposes only
Applications of nNOS antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin | |||
| Western Blotting | 1/250 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti nNOS recognizes a band of ~155 kDa by Western blot
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
|
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Esplugues, J.V. (2002) NO as a signalling molecule in the nervous system.
Br J Pharmacol. 135 (5): 1079-95
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