nNOS antibody

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Rabbit anti nNOS

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2402 P WB 50 µg
Rabbit anti nNOS antibody recognizes neuronal nitric oxide synthase also known as nNOS and NOS type I. nNOS catalyzes the synthesis of the second messenger molecule and neurotransmitter, nitric oxide (NO), in the brain. NO is also produced by immune cells, such as macrophages, as part of the immune response by the iNOS (inducible nitric oxide synthase). In endothelia, the molecule is produced by eNOS (endothelial nitric oxide synthase) where it acts as a vasodilator. Neuronal nitrogen oxide synthase, via regulation of NO levels, is important for nervous system development and plays a role in development and learning (Esplugues, 2002).

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to nNOS was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
50% Glycerol
Immunogen
Synthetic peptide corresponding to the sequence near the C-terminus of nNOS
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

UniProt
Q9Z0J4 Related reagents
Entrez Gene
Nos1 Related reagents
GO Terms
GO:0002028 regulation of sodium ion transport
GO:0004517 nitric-oxide synthase activity
GO:0043267 negative regulation of potassium ion transport
GO:0005516 calmodulin binding
GO:0005856 cytoskeleton
GO:0055114 oxidation-reduction process
GO:0006809 nitric oxide biosynthetic process
GO:0006941 striated muscle contraction
GO:0010181 FMN binding
GO:0018119 peptidyl-cysteine S-nitrosylation
GO:0020037 heme binding
GO:0035066 positive regulation of histone acetylation
GO:0042383 sarcolemma
GO:0042738 exogenous drug catabolic process
GO:0043197 dendritic spine
GO:0045202 synapse
GO:0045893 positive regulation of transcription, DNA-dependent
GO:0050660 flavin adenine dinucleotide binding
GO:0050661 NADP binding
GO:0051346 negative regulation of hydrolase activity
GO:0051612 negative regulation of serotonin uptake
GO:0051926 negative regulation of calcium ion transport
GO:0071363 cellular response to growth factor stimulus
Regulatory
For research purposes only

Applications of nNOS antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin
Western Blotting 1/250
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti nNOS recognizes a band of ~155 kDa by Western blot

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*