NFkB p65 antibody

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Rabbit anti NFkB p65

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2332 IC WB 50 µg
Rabbit anti NFkB p65 antibody recognizes Nuclear factor NF-kappa-B p65 subunit also known as Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 or RelA. NF-kappa-B p105 and p100 subunits are large precursor proteins that are cleaved by the ubiquitin/proteasome pathway into the mature NF-kappa-B p50 and p52 subunits (NF-kappa-B p105 cleavage results in the p50 unit, while cleavage of NF-kappa-B p100 results in the p52 subunit). Rel protein family members interact with p52 and p50 to form transcription factors. In the absence of stimulation an I-kappa-B protein mediated sequestration mechanism ensures that the transcription factors remain in the cytoplasm. Upon stimulation, I-kappa-B proteins become phosphorylated, which results in their ubiquitylation and subsequent degradation by the 26S proteasome.The NF-kappa-B pathway plays a critical in mediating immune responses after infection and has been implicated in a variety of diseases ranging from autoimmune disorders to cancer.

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
25% Glycerol
0.5% Bovine Serum Albumin
0.01% Thiomersal
Immunogen
Synthetic peptide surrounding amino acid 274 of mouse NFkB p65.
Approx. Protein Concentrations
IgG concentration 0.2 mg/ml

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
Q04207 Related reagents
Entrez Gene
Rela Related reagents
GO Terms
GO:0001889 liver development
GO:0001942 hair follicle development
GO:0003682 chromatin binding
GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
GO:0005829 cytosol
GO:0009887 organ morphogenesis
GO:0016563 transcription activator activity
GO:0019901 protein kinase binding
GO:0032495 response to muramyl dipeptide
GO:0042177 negative regulation of protein catabolic process
GO:0045084 positive regulation of interleukin-12 biosynthetic process
GO:0050727 regulation of inflammatory response
GO:0071532 ankyrin repeat binding
Regulatory
For research purposes only

Applications of NFkB p65 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunocytochemistry 10 20 ug/ml
Western Blotting 0.5 4.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti NFkB p65 antibody primarily recognizes a band of approximately 65 kDa in Jurkat cell lysate

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Product Specific References

References for NFkB p65 antibody

  1. Tran, T.H. et al. (2016) Repeated exposure to far infrared ray attenuates acute restraint stress in mice via inhibition of JAK2/STAT3 signaling pathway by induction of glutathione peroxidase-1.
    Neurochem Int. 94: 9-22.