MDM2 antibody

Rabbit anti Mouse MDM2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
MDM2

Product Code Applications Pack Size List Price Your Price Qty
AHP1329
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 50 µg loader
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loader
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Rabbit anti Mouse Mdm2 antibody detects murine Mdm2, a nuclear phosphoprotein that controls intracellular levels of the tumour suppressor protein p53 by ubiquitination and subsequent proteasomal degradation. Mdm2 also interacts with the retinoblastoma (RB) protein and E2F-1, to promote G1-S phase transition in the cell cycle.

Mdm2 activity is controlled by self-ubiquitination; however activation of the PI3K/PKB pathway leads to phosphorylation of Mdm2 at Ser185, which stabilises the protein.

Target Species
Mouse
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Mdm2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to aa 177-195 of mouse Mdm2.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/3000 1/12000
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1329 detects a band of approximately 90kDa in mouse MEF cell lysates.

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Further Reading

  1. Wienken, M. et al. (2017) Mdm2 as a chromatin modifier.
    J Mol Cell Biol. 9 (1): 74-80.
  2. Oliner, J.D. et al. (2016) The Role of MDM2 Amplification and Overexpression in Tumorigenesis.
    Cold Spring Harb Perspect Med.6(6):a026336.
  3. Hou, H. et al. (2019) The role of MDM2 amplification and overexpression in therapeutic resistance of malignant tumors.
    Cancer Cell Int. 19: 216.

RRID
AB_2143667
UniProt
P23804
Entrez Gene
Mdm2
GO Terms
GO:0002039 p53 binding
GO:0004842 ubiquitin-protein ligase activity
GO:0005737 cytoplasm
GO:0005730 nucleolus
GO:0007089 traversing start control point of mitotic cell cycle
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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