MCP-5 antibody

100% Secure

Rabbit anti Mouse MCP-5

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAM45 E FN WB 0.1 mg
Rabbit anti Mouse MCP-5 antibody recognizes mouse monocyte chemotactic protein 5 (MCP-5), also known as CCL12.

MCP-5 is a member of the beta-chemokine family and is strongly chemotactic for monocytes and peritoneal macrophages.

Product Details

Target Species
Mouse
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.1 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Antiserum Preparation
Antisera to mouse MCP-5 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant mouse MCP-5.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. For long term storage, the addition of 0.09% sodium azide is recommended.

N.B. For functional studies do not add azide.
Shelf Life
12 months from date of reconstitution.
Note
For long term storage, the addition of 0.09% sodium azide is recommended.

N.B. For functional studies do not add azide.

More Information

UniProt
Q62401 Related reagents
Entrez Gene
Ccl12 Related reagents
GO Terms
GO:0005615 extracellular space
GO:0006935 chemotaxis
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0008009 chemokine activity
Regulatory
For research purposes only

Applications of MCP-5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5ug/ml
Functional Assays 2.0ug/ml 3.0ug/ml
Western Blotting 0.1ug/ml 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*