Leptin antibody

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Rabbit anti Mouse Leptin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAM41 E WB 0.1 mg
Rabbit anti Mouse Leptin antibody recognizes mouse leptin.

Leptin (anti-obesity protein) is the product of the ob (obese) gene in mice and is involved in appetite control. The gene is expressed in adipose tissue and it is thought to be important in the regulation of fat stores. Mice of ob/ob genotype develop a form of diabetes similar to type II (non-insulin dependant) diabetes.

Product Details

Target Species
Mouse
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.1 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Antiserum Preparation
Antisera to mouse leptin were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant mouse leptin.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. For long term storage, the addition of 0.09% sodium azide is recommended.

N.B. For functional studies do not add azide.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P41160 Related reagents
Entrez Gene
Lep Related reagents
GO Terms
GO:0002021 response to dietary excess
GO:0005615 extracellular space
GO:0006006 glucose metabolic process
GO:0006111 regulation of gluconeogenesis
GO:0050796 regulation of insulin secretion
GO:0050810 regulation of steroid biosynthetic process
GO:0008083 growth factor activity
GO:0008203 cholesterol metabolic process
GO:0008206 bile acid metabolic process
GO:0008343 adult feeding behavior
GO:0021954 central nervous system neuron development
GO:0030073 insulin secretion
GO:0030300 regulation of intestinal cholesterol absorption
GO:0032099 negative regulation of appetite
GO:0032868 response to insulin stimulus
GO:0042445 hormone metabolic process
GO:0042755 eating behavior
GO:0045639 positive regulation of myeloid cell differentiation
Regulatory
For research purposes only

Applications of Leptin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5ug/ml
Western Blotting 0.1ug/ml 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*