IL-6 antibody

Rabbit anti Mouse Interleukin-6

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IL-6

Product Code Applications Pack Size List Price Your Price Qty
AAM15G
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SDS Safety Datasheet SDS
E FN WB 0.1 mg loader
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Rabbit anti Mouse Interleukin-6 antibody recognizes mouse Interleukin-6 (IL-6). Rabbit anti Mouse Interleukin-6 antibody neutralizes murine IL-6 activity with an ND50 for mIL-6 (3 pgml-1 of between 0.24 and 0.36μg ml-1.

Target Species
Mouse
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antisera to murine interleukin 6 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by antigen affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant murine IL-6.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Functional Assays 5.0 8.0ng/ml
Western Blotting 0.1 0.2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Western Blotting
This purified mouse IL-6 antibody may used in Western Blotting applications under either reducing or non-reducing conditions.

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RRID
AB_323581
UniProt
P08505
Entrez Gene
Il6
GO Terms
GO:0001781 neutrophil apoptosis
GO:0006953 acute-phase response
GO:0005125 cytokine activity
GO:0005138 interleukin-6 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0009897 external side of plasma membrane
GO:0031018 endocrine pancreas development
GO:0042832 defense response to protozoan
GO:0045079 negative regulation of chemokine biosynthetic process
GO:0045630 positive regulation of T-helper 2 cell differentiation
GO:0045893 positive regulation of transcription, DNA-dependent
GO:0046716 muscle cell homeostasis
GO:0046888 negative regulation of hormone secretion
GO:0050679 positive regulation of epithelial cell proliferation
GO:0060445 branching involved in salivary gland morphogenesis
GO:0060664 epithelial cell proliferation involved in salivary gland morphogenesis
GO:0070091 glucagon secretion
GO:0070102 interleukin-6-mediated signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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