IL-6 antibody
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Rabbit anti Mouse Interleukin-6
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IL-6
Antibody Quality and Performance Guarantee
| Rabbit anti Mouse Interleukin-6 antibody recognizes mouse Interleukin-6 (IL-6). Rabbit anti Mouse Interleukin-6 antibody neutralizes murine IL-6 activity with an ND50 for mIL-6 (3 pgml-1 of between 0.24 and 0.36μg ml-1. |
- Target Species
- Mouse
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide - Antiserum Preparation
- Antisera to murine interleukin 6 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by antigen affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant murine IL-6.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5 | 2.0ug/ml |
| Functional Assays | |
5.0 | 8.0ng/ml |
| Western Blotting | |
0.1 | 0.2ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
- Western Blotting
- This purified mouse IL-6 antibody may used in Western Blotting applications under either reducing or non-reducing conditions.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
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| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Description | Sheep anti Rabbit IgG:FITC | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml | Log in | |||
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| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
- RRID
- AB_323581
- UniProt
- P08505
- Entrez Gene
- Il6
- GO Terms
- GO:0001781 neutrophil apoptosis
- GO:0006953 acute-phase response
- GO:0005125 cytokine activity
- GO:0005138 interleukin-6 receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008083 growth factor activity
- GO:0009897 external side of plasma membrane
- GO:0031018 endocrine pancreas development
- View More GO Terms
- GO:0042832 defense response to protozoan
- GO:0045079 negative regulation of chemokine biosynthetic process
- GO:0045630 positive regulation of T-helper 2 cell differentiation
- GO:0045893 positive regulation of transcription, DNA-dependent
- GO:0046716 muscle cell homeostasis
- GO:0046888 negative regulation of hormone secretion
- GO:0050679 positive regulation of epithelial cell proliferation
- GO:0060445 branching involved in salivary gland morphogenesis
- GO:0060664 epithelial cell proliferation involved in salivary gland morphogenesis
- GO:0070091 glucagon secretion
- GO:0070102 interleukin-6-mediated signaling pathway
AAM15G
146189
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