IL-3 antibody

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Rabbit anti Mouse Interleukin-3

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AAM14G
Datasheet
E FN WB
MSDS
0.1 mg loader

Rabbit anti Mouse Interleukin-3 antibody recognizes mouse Interleukin-3 (IL-3).Rabbit anti Mouse Interleukin-3 antibody neutralizes murine IL-3 activity with an ND50 for mIL-3 (0.5ngml-1 of between 0.15 and 0.2μg ml-1.

Product Details

Target Species
Mouse
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 1.0 ml sterile PBS containing 0.1% Bovine Serum Albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.

The addition of 0.09% sodium azide is recommended for long term storage.
Antiserum Preparation
Antisera to murine interleukin 3 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by antigen affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Highly purified recombinant murine IL-3.
Approx. Protein Concentrations
IgG concentration 1 mg/ml

Storage Information

Storage
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P01586
Entrez Gene
Il3
GO Terms
GO:0002763 positive regulation of myeloid leukocyte differentiation
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0010507 negative regulation of autophagy
GO:0019221 cytokine-mediated signaling pathway
GO:0033026 negative regulation of mast cell apoptosis
GO:0033028 myeloid cell apoptosis
GO:0045885 positive regulation of survival gene product expression
GO:0046330 positive regulation of JNK cascade
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0070668 positive regulation of mast cell proliferation
Regulatory
For research purposes only

Applications of IL-3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 ug/ml
Functional Assays 0.15ug/ml 0.20ug/ml
Western Blotting 0.1ug/ml 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml