IL-3 antibody
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Rabbit anti Mouse Interleukin-3 antibody recognizes mouse Interleukin-3 (IL-3).Rabbit anti Mouse Interleukin-3 antibody neutralizes murine IL-3 activity with an ND50 for mIL-3 (0.5ngml-1 of between 0.15 and 0.2μg ml-1.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 1.0 ml sterile PBS containing 0.1% Bovine Serum Albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
The addition of 0.09% sodium azide is recommended for long term storage. - Antiserum Preparation
- Antisera to murine interleukin 3 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by antigen affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Highly purified recombinant murine IL-3.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
Storage Information
- Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- P01586 Related reagents
- Entrez Gene
- Il3 Related reagents
- GO Terms
- GO:0002763 positive regulation of myeloid leukocyte differentiation
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008083 growth factor activity
- GO:0010507 negative regulation of autophagy
- GO:0019221 cytokine-mediated signaling pathway
- GO:0033026 negative regulation of mast cell apoptosis
- GO:0033028 myeloid cell apoptosis
- GO:0045885 positive regulation of survival gene product expression
- GO:0046330 positive regulation of JNK cascade
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- GO:0070668 positive regulation of mast cell proliferation
- Regulatory
- For research purposes only
Applications of IL-3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.5 ug/ml | ||
| Functional Assays | 0.15ug/ml | 0.20ug/ml | |
| Western Blotting | 0.1ug/ml | 0.2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | 0.5 ml | WB* |
